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Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16.

Human enterovirus 71 (HEV71) and coxsackievirus A16 (CVA16) are two major aetiological agents of hand, foot and mouth disease (HFMD) in children. Recently there have been several large outbreaks of HFMD in Vietnam and the Asia-Pacific region. In this study, a multiplex RT-PCR assay was developed in...

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Main Authors:	Thao, Nguyen Thi Thanh, Ngoc, Nguyen Thi Kim, Tú, Phan Van, Thúy, Tr`an Thi, Cardosa, Mary Jane, McMinn, P.C, Phuektes, Patchara
Format:	E-Article
Language:	English
Published:	Elsevier 2010
Subjects:	Q Science (General) QR355 Virology R Medicine (General)
Online Access:	http://ir.unimas.my/id/eprint/7027/1/Development%20of%20a%20multiplex%20polymerase%20chain%20reaction%20assay%20for%20simultaneous%20identification%20of%20human%20enterovirus%2071%20and%20coxsackievirus%20A16..pdf http://ir.unimas.my/id/eprint/7027/ http://www.sciencedirect.com/science/article/pii/S0166093410003307
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spelling	my.unimas.ir.70272015-04-14T07:37:37Z http://ir.unimas.my/id/eprint/7027/ Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16. Thao, Nguyen Thi Thanh Ngoc, Nguyen Thi Kim Tú, Phan Van Thúy, Tr`an Thi Cardosa, Mary Jane McMinn, P.C Phuektes, Patchara Q Science (General) QR355 Virology R Medicine (General) Human enterovirus 71 (HEV71) and coxsackievirus A16 (CVA16) are two major aetiological agents of hand, foot and mouth disease (HFMD) in children. Recently there have been several large outbreaks of HFMD in Vietnam and the Asia-Pacific region. In this study, a multiplex RT-PCR assay was developed in order to detect simultaneously HEV71, CVA16 and other human enteroviruses. Enterovirus detection was performed with a mixture of three pairs of oligonucleotide primers: one pair of published primers for amplifying all known enterovirus genomes and two new primer pairs specific for detection of the VP1 genes of HEV71 and CVA16. Enterovirus isolates, CVA16 and HEV71 strains identified previously from patients with HFMD were examined to evaluate the sensitivity and specificity of the multiplex RT-PCR assay. The assay was then applied to the direct detection of these viruses in clinical specimens obtained from HFMD cases identified at Children’s Hospital Number 2, Ho Chi Minh City, Vietnam. The multiplex RT-PCR assay showed 100% specificity in screening for enteroviruses and in identifying HEV71 and CVA16. Similar results were obtained when using the multiplex RT-PCR assay to screen for enteroviruses and to identify HEV71 and CVA16 in clinical specimens obtained from HFMD cases identified at the hospital. This multiplex RT-PCR assay is a rapid, sensitive and specific assay for the diagnosis of HEV71 or CVA16 infection in cases of HFMD and is also potentially useful for molecular epidemiological investigations. Elsevier 2010-09 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/7027/1/Development%20of%20a%20multiplex%20polymerase%20chain%20reaction%20assay%20for%20simultaneous%20identification%20of%20human%20enterovirus%2071%20and%20coxsackievirus%20A16..pdf Thao, Nguyen Thi Thanh and Ngoc, Nguyen Thi Kim and Tú, Phan Van and Thúy, Tr`an Thi and Cardosa, Mary Jane and McMinn, P.C and Phuektes, Patchara (2010) Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16. Journal of Virological Methods, 170. pp. 134-139. ISSN 0166-0934 http://www.sciencedirect.com/science/article/pii/S0166093410003307 doi:10.1016/j.jviromet.2010.09.017
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	Q Science (General) QR355 Virology R Medicine (General)
spellingShingle	Q Science (General) QR355 Virology R Medicine (General) Thao, Nguyen Thi Thanh Ngoc, Nguyen Thi Kim Tú, Phan Van Thúy, Tr`an Thi Cardosa, Mary Jane McMinn, P.C Phuektes, Patchara Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16.
description	Human enterovirus 71 (HEV71) and coxsackievirus A16 (CVA16) are two major aetiological agents of hand, foot and mouth disease (HFMD) in children. Recently there have been several large outbreaks of HFMD in Vietnam and the Asia-Pacific region. In this study, a multiplex RT-PCR assay was developed in order to detect simultaneously HEV71, CVA16 and other human enteroviruses. Enterovirus detection was performed with a mixture of three pairs of oligonucleotide primers: one pair of published primers for amplifying all known enterovirus genomes and two new primer pairs specific for detection of the VP1 genes of HEV71 and CVA16. Enterovirus isolates, CVA16 and HEV71 strains identified previously from patients with HFMD were examined to evaluate the sensitivity and specificity of the multiplex RT-PCR assay. The assay was then applied to the direct detection of these viruses in clinical specimens obtained from HFMD cases identified at Children’s Hospital Number 2, Ho Chi Minh City, Vietnam. The multiplex RT-PCR assay showed 100% specificity in screening for enteroviruses and in identifying HEV71 and CVA16. Similar results were obtained when using the multiplex RT-PCR assay to screen for enteroviruses and to identify HEV71 and CVA16 in clinical specimens obtained from HFMD cases identified at the hospital. This multiplex RT-PCR assay is a rapid, sensitive and specific assay for the diagnosis of HEV71 or CVA16 infection in cases of HFMD and is also potentially useful for molecular epidemiological investigations.
format	E-Article
author	Thao, Nguyen Thi Thanh Ngoc, Nguyen Thi Kim Tú, Phan Van Thúy, Tr`an Thi Cardosa, Mary Jane McMinn, P.C Phuektes, Patchara
author_facet	Thao, Nguyen Thi Thanh Ngoc, Nguyen Thi Kim Tú, Phan Van Thúy, Tr`an Thi Cardosa, Mary Jane McMinn, P.C Phuektes, Patchara
author_sort	Thao, Nguyen Thi Thanh
title	Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16.
title_short	Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16.
title_full	Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16.
title_fullStr	Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16.
title_full_unstemmed	Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16.
title_sort	development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus a16.
publisher	Elsevier
publishDate	2010
url	http://ir.unimas.my/id/eprint/7027/1/Development%20of%20a%20multiplex%20polymerase%20chain%20reaction%20assay%20for%20simultaneous%20identification%20of%20human%20enterovirus%2071%20and%20coxsackievirus%20A16..pdf http://ir.unimas.my/id/eprint/7027/ http://www.sciencedirect.com/science/article/pii/S0166093410003307
_version_	1644510194056560640
score	13.211869

Development of a multiplex polymerase chain reaction assay for simultaneous identification of human enterovirus 71 and coxsackievirus A16.

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