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A generic assay for whole-genome amplification and deep sequencing of enterovirus A71

sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Understanding EV-A71 population dynamics is therefore essential for understanding this emerging infec...

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書誌詳細
主要な著者: Le Van, Tan, Nguyen, Thi Kim Tuyen, Tran, Tan Thanh, Tran, Thuy Ngan, Hoang, Minh Tu Van, Saraswathy, Sabanathan, Tran, Thi My Van, Le, Thi My Thanh, Lam, Anh Nguyet, Jemma L., Geoghegan, Ong, Kien Chai, David, Perera, Vu, Thi Ty Hang, Nguyen, Thi Han Ny, Nguyen, To Anh, Do, Quang Ha, Phan, Tu Qui, Do, Chau Viet, Ha, Manh Tuan, Wong, Kum Thong, Holmes, Edward C., Nguyen, Van Vinh Chau, Thwaites, Guy, H. Rogier, van Doorn
フォーマット: 論文
言語:English
出版事項: Elsevier 2015
主題:
Q Science (General)
R Medicine (General)
オンライン･アクセス:http://ir.unimas.my/id/eprint/6562/1/A%20generic%20assay.pdf
http://ir.unimas.my/id/eprint/6562/
http://www.sciencedirect.com/science/article/pii/S0166093415000336#
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その他の書誌記述
要約:sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Understanding EV-A71 population dynamics is therefore essential for understanding this emerging infection, and may provide pivotal information for vaccine development. Despite the public health burden of EV-A71, relatively few EV-A71 complete-genome sequences are available for analysis and from limited geographical localities. The availability of an efficient procedure for whole-genome sequencing would stimulate effort to generate more viral sequence data. Herein, we report for the first time the development of a next-generation sequencing based protocol for whole-genome sequencing of EV-A71 directly from clinical specimens. We were able to sequence viruses of subgenogroup C4 and B5, while RNA from culture materials of diverse EV-A71 subgenogroups belonging to both genogroup B and C was successfully amplified. The nature of intra-host genetic diversity was explored in 22 clinical samples, revealing 107 positions carrying minor variants (ranging from 0 to 15 variants per sample). Our analysis of EVA71 strains sampled in 2013 showed that they all belonged to subgenogroup B5, representing the first report of this subgenogroup in Vietnam. In conclusion, we have successfully developed a high-throughput next-generation sequencing-based assay for whole-genome sequencing of EV-A71 from clinical samples

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