Bioethanol production from office waste using Saccharomyces cerevisiae via simultaneous saccharification fermentation (SSF)

Bioethanol is an attractive and sustainable energy source to fuel transportation. It is seen as a good alternative fuel as it emits zero net carbon output into the atmosphere. In this study, bioethanol was produced from office waste by using Saccharomyces cerevisiae. Prior to the laboratory experi...

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Bibliographic Details
Main Author: Nor Hasni, Binti Hambali
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
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Online Access:http://ir.unimas.my/id/eprint/6047/4/Nor%20Hasni%20Binti%20Hambali%20ft.pdf
http://ir.unimas.my/id/eprint/6047/
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Summary:Bioethanol is an attractive and sustainable energy source to fuel transportation. It is seen as a good alternative fuel as it emits zero net carbon output into the atmosphere. In this study, bioethanol was produced from office waste by using Saccharomyces cerevisiae. Prior to the laboratory experimentations, a mini survey was conducted to collect and determine the approximate amount of the office waste being used weekly by respondents. Simultaneous saccharification and fermentation (SSF) experiments were then performed to utilize the enzymatic bond breaking of the office waste into fermentable sugar in order to produce ethanol. The final fermentation products were analysed via DNS reducing sugar assay and phenol-sulphuric total carbohydrate assays and High Performance Liquid Chromatography (HPLC). This study has shown that office waste can be converted to bioethanol from S. cerevisiae via SSF. From the result obtained, 5% for 50 FPU/g paper give an effective production of ethanol which is 70.99% as it utilized the glucose faster with low amount of lactic acid and acetic acid.