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Antimicrobial activity of crude extract from antibiotic producing fungi

Fungi have been used for producing medically useful compound. Although there are a large number of fungi species, only a relative few have been found to produce antibiotics. Nowadays, the bacteria become resistance toward antibiotics because of overuse of the antibiotics in the treatment of bacteria...

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Main Author: Norlaila, binti Deraman
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
Subjects:
Q Science (General)
S Agriculture (General)
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/6042/8/ANTIMICROBIAL%20ACTIVITY%20OF%20CRUDE%20EXTRACT%20FROM%20ANTIBIOTIC%20PRODUCING%20FUNGI%28OCR%29.pdf
http://ir.unimas.my/id/eprint/6042/
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spelling my.unimas.ir.60422023-11-15T03:18:49Z http://ir.unimas.my/id/eprint/6042/ Antimicrobial activity of crude extract from antibiotic producing fungi Norlaila, binti Deraman Q Science (General) S Agriculture (General) SB Plant culture Fungi have been used for producing medically useful compound. Although there are a large number of fungi species, only a relative few have been found to produce antibiotics. Nowadays, the bacteria become resistance toward antibiotics because of overuse of the antibiotics in the treatment of bacterial infection. The objectives of this study are to determine antimicrobial activity of crude extracts from antibiotic producing fungi and to compare the effectiveness of antibacterial properties among four different crude extracts using three different assays. Evaluation on antimicrobial activity was done on crude extract using four different solvents and five different concentrations. The solvents involved in this research including hexane, dichloromethane (DCM), ethyl acetate, and methanol. The concentration used were 1.0 mg/µl, 0.5 mg/µl, 0.25 mg/µl,0.125 mg/µl and 0.0623 mg/µl. Antimicrobial activity of crude extracts was determined using different bacteria which are three Gram-negative bacteria, Escherichia coli, Salmonella typhi, Enterobacter aerogenes and one Gram-positive bacteria, Staphylococcus aureas. The disc diffusion assay, broth microdilution assay and direct bioautographic assay was the method used to determine antimicrobial activity using the crude extracts from the antibiotic producing fungi. All of the crude extracts showed different antimicrobial activity toward different bacteria. From the result hexane and ethyl acetate extracts showed active against Gram negative bacteria, E. aerogenes and Gram positive bacteria S. aureas. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/6042/8/ANTIMICROBIAL%20ACTIVITY%20OF%20CRUDE%20EXTRACT%20FROM%20ANTIBIOTIC%20PRODUCING%20FUNGI%28OCR%29.pdf Norlaila, binti Deraman (2012) Antimicrobial activity of crude extract from antibiotic producing fungi. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
S Agriculture (General)
SB Plant culture
spellingShingle Q Science (General)
S Agriculture (General)
SB Plant culture
Norlaila, binti Deraman
Antimicrobial activity of crude extract from antibiotic producing fungi
description Fungi have been used for producing medically useful compound. Although there are a large number of fungi species, only a relative few have been found to produce antibiotics. Nowadays, the bacteria become resistance toward antibiotics because of overuse of the antibiotics in the treatment of bacterial infection. The objectives of this study are to determine antimicrobial activity of crude extracts from antibiotic producing fungi and to compare the effectiveness of antibacterial properties among four different crude extracts using three different assays. Evaluation on antimicrobial activity was done on crude extract using four different solvents and five different concentrations. The solvents involved in this research including hexane, dichloromethane (DCM), ethyl acetate, and methanol. The concentration used were 1.0 mg/µl, 0.5 mg/µl, 0.25 mg/µl,0.125 mg/µl and 0.0623 mg/µl. Antimicrobial activity of crude extracts was determined using different bacteria which are three Gram-negative bacteria, Escherichia coli, Salmonella typhi, Enterobacter aerogenes and one Gram-positive bacteria, Staphylococcus aureas. The disc diffusion assay, broth microdilution assay and direct bioautographic assay was the method used to determine antimicrobial activity using the crude extracts from the antibiotic producing fungi. All of the crude extracts showed different antimicrobial activity toward different bacteria. From the result hexane and ethyl acetate extracts showed active against Gram negative bacteria, E. aerogenes and Gram positive bacteria S. aureas.
format Final Year Project Report
author Norlaila, binti Deraman
author_facet Norlaila, binti Deraman
author_sort Norlaila, binti Deraman
title Antimicrobial activity of crude extract from antibiotic producing fungi
title_short Antimicrobial activity of crude extract from antibiotic producing fungi
title_full Antimicrobial activity of crude extract from antibiotic producing fungi
title_fullStr Antimicrobial activity of crude extract from antibiotic producing fungi
title_full_unstemmed Antimicrobial activity of crude extract from antibiotic producing fungi
title_sort antimicrobial activity of crude extract from antibiotic producing fungi
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2012
url http://ir.unimas.my/id/eprint/6042/8/ANTIMICROBIAL%20ACTIVITY%20OF%20CRUDE%20EXTRACT%20FROM%20ANTIBIOTIC%20PRODUCING%20FUNGI%28OCR%29.pdf
http://ir.unimas.my/id/eprint/6042/
_version_ 1783883452331851776
score 13.211869

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