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A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones

Theobroma cacao L. is currently in the third ranking important commodity in Malaysia after oil palm and rubber. As to increase the best quality of cocoa production with faster propagation, the study of tissue culture for cocoa was carried out. The objectives of this study were to compare between two...

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Main Author: Norhashimah, Binti Abdul Razak
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2012
Subjects:
S Agriculture (General)
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/6034/4/Norhashimah%20Binti%20Abdul%20Razak%20ft.pdf
http://ir.unimas.my/id/eprint/6034/
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spelling my.unimas.ir.60342023-11-14T08:23:20Z http://ir.unimas.my/id/eprint/6034/ A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones Norhashimah, Binti Abdul Razak S Agriculture (General) SB Plant culture Theobroma cacao L. is currently in the third ranking important commodity in Malaysia after oil palm and rubber. As to increase the best quality of cocoa production with faster propagation, the study of tissue culture for cocoa was carried out. The objectives of this study were to compare between two protocols for three selected cocoa clones, to discover the best protocol for better multiplication of cocoa propagation and to compare which cocoa clones give best response in somatic embryogenesis. This study is important in order to discover the best protocol to optimize the production of somatic embryos. The cocoa clones, KKM 1, MCB C5 and MCB C8 were studied in this experiment. Two protocols for somatic embryogenesis, Pennsylvania State protocol and Nestle protocol were compared in this study. The staminodes cultures were established based on Pennsylvania State and Nestle protocol for comparison of somatic embryogenesis. Based on the result, the clone for high propagation via somatic embryogenesis was cocoa clone MCB C8 (Nestle Protocol) under 10% Clorox® treatment with 82.7% of primary somatic embryos and 90 total explants producing secondary somatic embryos. Pennsylvania State Protocol was the best protocol to induce the somatic embryos for all selected cocoa clones. Universiti Malaysia Sarawak, (UNIMAS) 2012 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/6034/4/Norhashimah%20Binti%20Abdul%20Razak%20ft.pdf Norhashimah, Binti Abdul Razak (2012) A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
SB Plant culture
spellingShingle S Agriculture (General)
SB Plant culture
Norhashimah, Binti Abdul Razak
A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones
description Theobroma cacao L. is currently in the third ranking important commodity in Malaysia after oil palm and rubber. As to increase the best quality of cocoa production with faster propagation, the study of tissue culture for cocoa was carried out. The objectives of this study were to compare between two protocols for three selected cocoa clones, to discover the best protocol for better multiplication of cocoa propagation and to compare which cocoa clones give best response in somatic embryogenesis. This study is important in order to discover the best protocol to optimize the production of somatic embryos. The cocoa clones, KKM 1, MCB C5 and MCB C8 were studied in this experiment. Two protocols for somatic embryogenesis, Pennsylvania State protocol and Nestle protocol were compared in this study. The staminodes cultures were established based on Pennsylvania State and Nestle protocol for comparison of somatic embryogenesis. Based on the result, the clone for high propagation via somatic embryogenesis was cocoa clone MCB C8 (Nestle Protocol) under 10% Clorox® treatment with 82.7% of primary somatic embryos and 90 total explants producing secondary somatic embryos. Pennsylvania State Protocol was the best protocol to induce the somatic embryos for all selected cocoa clones.
format Final Year Project Report
author Norhashimah, Binti Abdul Razak
author_facet Norhashimah, Binti Abdul Razak
author_sort Norhashimah, Binti Abdul Razak
title A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones
title_short A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones
title_full A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones
title_fullStr A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones
title_full_unstemmed A comparison of production of somatic embryo from three selected cocoa (Theobroma Cacao L.) clones
title_sort comparison of production of somatic embryo from three selected cocoa (theobroma cacao l.) clones
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2012
url http://ir.unimas.my/id/eprint/6034/4/Norhashimah%20Binti%20Abdul%20Razak%20ft.pdf
http://ir.unimas.my/id/eprint/6034/
_version_ 1783883451976384512
score 13.211869

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