Cover Image

Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba)

Neolamarckia cadamba or locally known as Kelampayan is an evergreen and tropical tree native to South Asia under the family of Rubiaceae. Kelampayan is the best raw material for plywood industry and also used as raw materials for pulp and paper industry. Arabinogalactan Proteins (AGPs) are extracell...

Full description

Saved in:
Bibliographic Details
Main Author: Tang, Leh Ee
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
QK Botany
Online Access:http://ir.unimas.my/id/eprint/5343/1/Leh%20Ee%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/5343/3/Tang%20Leh%20Ee.pdf
http://ir.unimas.my/id/eprint/5343/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.5343
record_format eprints
spelling my.unimas.ir.53432024-05-02T08:51:15Z http://ir.unimas.my/id/eprint/5343/ Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba) Tang, Leh Ee QK Botany Neolamarckia cadamba or locally known as Kelampayan is an evergreen and tropical tree native to South Asia under the family of Rubiaceae. Kelampayan is the best raw material for plywood industry and also used as raw materials for pulp and paper industry. Arabinogalactan Proteins (AGPs) are extracellular proteoglycans that are implicated in plant growth and development. Tubulin is a major cytoskeleton protein and as a building block of microtubules. The cytoskeleton is important in plant cell shapes, cell division and cell transport. The main objective of this study was to identify the single nucleotide variation in the AGP and beta-tubulin gene of kelampayan. Genomic DNA was firstly extracted from six N. cadamba trees. Targeted DNA sequence of arabinogalactan (AGP) gene and beta tubulin (β-TUB) were amplified by Polymerase Chain Reaction (PCR) by using primer set. The 340 bp of AGP and 491 bp of β-TUB amplicons were subjected to BLASTn analysis to carry out the sequence homology search in the NCBI. Sequence alignment then was carried out by using ClustalW software for single nucleotide polymorphism (SNP) detection. One sequence variation within arabinogalactan gene which was caused by single nucleotide substitution was detected. Five sequence variations and one InDel polymorphism within beta tubulin were detected. The finding of SNPs in AGP and β-TUB was very important as a valuable marker in molecular selective breeding. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/5343/1/Leh%20Ee%20%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/5343/3/Tang%20Leh%20Ee.pdf Tang, Leh Ee (2011) Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba). [Final Year Project Report]
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic QK Botany
spellingShingle QK Botany
Tang, Leh Ee
Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba)
description Neolamarckia cadamba or locally known as Kelampayan is an evergreen and tropical tree native to South Asia under the family of Rubiaceae. Kelampayan is the best raw material for plywood industry and also used as raw materials for pulp and paper industry. Arabinogalactan Proteins (AGPs) are extracellular proteoglycans that are implicated in plant growth and development. Tubulin is a major cytoskeleton protein and as a building block of microtubules. The cytoskeleton is important in plant cell shapes, cell division and cell transport. The main objective of this study was to identify the single nucleotide variation in the AGP and beta-tubulin gene of kelampayan. Genomic DNA was firstly extracted from six N. cadamba trees. Targeted DNA sequence of arabinogalactan (AGP) gene and beta tubulin (β-TUB) were amplified by Polymerase Chain Reaction (PCR) by using primer set. The 340 bp of AGP and 491 bp of β-TUB amplicons were subjected to BLASTn analysis to carry out the sequence homology search in the NCBI. Sequence alignment then was carried out by using ClustalW software for single nucleotide polymorphism (SNP) detection. One sequence variation within arabinogalactan gene which was caused by single nucleotide substitution was detected. Five sequence variations and one InDel polymorphism within beta tubulin were detected. The finding of SNPs in AGP and β-TUB was very important as a valuable marker in molecular selective breeding.
format Final Year Project Report
author Tang, Leh Ee
author_facet Tang, Leh Ee
author_sort Tang, Leh Ee
title Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba)
title_short Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba)
title_full Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba)
title_fullStr Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba)
title_full_unstemmed Sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (Neolamarckia cadamba)
title_sort sequence polymorphism of arabinogalactan and beta tubulin gene in kelampayan (neolamarckia cadamba)
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2011
url http://ir.unimas.my/id/eprint/5343/1/Leh%20Ee%20%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/5343/3/Tang%20Leh%20Ee.pdf
http://ir.unimas.my/id/eprint/5343/
_version_ 1800728054899671040
score 13.211869

Similar Items