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Profiling of total fatty acid content during jatropha seed development

Jatropha curcas is a potential source of vegetable oil in production of biodiesel. Enzyme such as stearoyl-acyl carrier protein desaturase (ACP) plays major role in catalyzing production of monounsaturated fatty acid that important for a good biodiesel characteristics. This experiment is conducted...

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Main Author: Nur Azlan, Bin Yusuf
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/5341/7/Profiling%20of%20Total%20Fatty%20Acid%20Content%20During%20Jatropha%20Seed%20Development%28OCR%29dlm%20IR.pdf
http://ir.unimas.my/id/eprint/5341/
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spelling my.unimas.ir.53412023-11-29T03:46:03Z http://ir.unimas.my/id/eprint/5341/ Profiling of total fatty acid content during jatropha seed development Nur Azlan, Bin Yusuf Q Science (General) Jatropha curcas is a potential source of vegetable oil in production of biodiesel. Enzyme such as stearoyl-acyl carrier protein desaturase (ACP) plays major role in catalyzing production of monounsaturated fatty acid that important for a good biodiesel characteristics. This experiment is conducted to investigate which period is the most valuable for the seed of J. curcas actively synthesizing fatty acids. This involves oil extraction of seeds from different intervals of seed development after anthesis and analysis with Proton Nuclear Magnetic Resonance (H-NMR) and Fourier Transform Infrared Spectrometry (FT-IR). Seed of yellow fruit has the highest oil content and most stages of fruit possessed unsaturated fatty acids. The highest concentration of unsaturated fatty acid is at stage IV (ripe yellow fruit). The presence of Fatty Acid Methyl Ester (FAME) can be detected at 3.81 ppm signal under H-NMR verification after transesterification with potassium hydroxide (KOH)-catalyzed methanolysis without reflux method with little modifications on original protocol. This is important for further analysis through Gas Chromatography (GC) to determine the percentage composition of fatty acid. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/5341/7/Profiling%20of%20Total%20Fatty%20Acid%20Content%20During%20Jatropha%20Seed%20Development%28OCR%29dlm%20IR.pdf Nur Azlan, Bin Yusuf (2011) Profiling of total fatty acid content during jatropha seed development. [Final Year Project Report]
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Nur Azlan, Bin Yusuf
Profiling of total fatty acid content during jatropha seed development
description Jatropha curcas is a potential source of vegetable oil in production of biodiesel. Enzyme such as stearoyl-acyl carrier protein desaturase (ACP) plays major role in catalyzing production of monounsaturated fatty acid that important for a good biodiesel characteristics. This experiment is conducted to investigate which period is the most valuable for the seed of J. curcas actively synthesizing fatty acids. This involves oil extraction of seeds from different intervals of seed development after anthesis and analysis with Proton Nuclear Magnetic Resonance (H-NMR) and Fourier Transform Infrared Spectrometry (FT-IR). Seed of yellow fruit has the highest oil content and most stages of fruit possessed unsaturated fatty acids. The highest concentration of unsaturated fatty acid is at stage IV (ripe yellow fruit). The presence of Fatty Acid Methyl Ester (FAME) can be detected at 3.81 ppm signal under H-NMR verification after transesterification with potassium hydroxide (KOH)-catalyzed methanolysis without reflux method with little modifications on original protocol. This is important for further analysis through Gas Chromatography (GC) to determine the percentage composition of fatty acid.
format Final Year Project Report
author Nur Azlan, Bin Yusuf
author_facet Nur Azlan, Bin Yusuf
author_sort Nur Azlan, Bin Yusuf
title Profiling of total fatty acid content during jatropha seed development
title_short Profiling of total fatty acid content during jatropha seed development
title_full Profiling of total fatty acid content during jatropha seed development
title_fullStr Profiling of total fatty acid content during jatropha seed development
title_full_unstemmed Profiling of total fatty acid content during jatropha seed development
title_sort profiling of total fatty acid content during jatropha seed development
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2011
url http://ir.unimas.my/id/eprint/5341/7/Profiling%20of%20Total%20Fatty%20Acid%20Content%20During%20Jatropha%20Seed%20Development%28OCR%29dlm%20IR.pdf
http://ir.unimas.my/id/eprint/5341/
_version_ 1783951119408431104
score 13.211869

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