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Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization

Some species in the genus Pseudo-nitzschia have the ability to produce domoic acid (DA). DA is a potent neurotoxin responsible for a human intoxication known as amnesic shellfish poisoning (ASP). Identification of the species mainly depends on the morphological observation which is insufficient to...

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Bibliographic Details
Main Author: Suk Hui, Voon
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
Q Science (General)
QD Chemistry
Online Access:http://ir.unimas.my/id/eprint/5337/8/Voon%20Suk%20Hui.pdf
http://ir.unimas.my/id/eprint/5337/
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spelling my.unimas.ir.53372024-02-09T03:16:06Z http://ir.unimas.my/id/eprint/5337/ Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization Suk Hui, Voon Q Science (General) QD Chemistry Some species in the genus Pseudo-nitzschia have the ability to produce domoic acid (DA). DA is a potent neurotoxin responsible for a human intoxication known as amnesic shellfish poisoning (ASP). Identification of the species mainly depends on the morphological observation which is insufficient to discriminate to the species level. Species identification of Pseudo-nitzschia acquires application of inclusive electron microscopy to investigate the fine ultrastructures of frustules. In this study, a molecular approach was applied to identify the harmful Pseudo-nitzschia species by using the whole-cell fluorescence in situ hybridization (FISH). The rRNA-targeted oligonucleotide probes were successfully designed in silico targeting P. pungens, P. brasilina and P. caciantha. The P. pungens species-specific probe was tested using Pseudo-nitzschia culture samples. The results showed high specificity and accessibility of the probe. The probe was further tested on natural seawater samples with cell spiking. Our results indicated that the fluorescent oligonucleotide probes (PuD1) show great promise as a tool that can facilitate the detection of P. pungens in the phytoplankton samples. The whole-cell FISH method was proven as a rapid detection tool for Pseudonitzschia with high specificity. This approach is suggested to be adopted in the country harmful algae monitoring and mitigation. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/5337/8/Voon%20Suk%20Hui.pdf Suk Hui, Voon (2011) Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QD Chemistry
spellingShingle Q Science (General)
QD Chemistry
Suk Hui, Voon
Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization
description Some species in the genus Pseudo-nitzschia have the ability to produce domoic acid (DA). DA is a potent neurotoxin responsible for a human intoxication known as amnesic shellfish poisoning (ASP). Identification of the species mainly depends on the morphological observation which is insufficient to discriminate to the species level. Species identification of Pseudo-nitzschia acquires application of inclusive electron microscopy to investigate the fine ultrastructures of frustules. In this study, a molecular approach was applied to identify the harmful Pseudo-nitzschia species by using the whole-cell fluorescence in situ hybridization (FISH). The rRNA-targeted oligonucleotide probes were successfully designed in silico targeting P. pungens, P. brasilina and P. caciantha. The P. pungens species-specific probe was tested using Pseudo-nitzschia culture samples. The results showed high specificity and accessibility of the probe. The probe was further tested on natural seawater samples with cell spiking. Our results indicated that the fluorescent oligonucleotide probes (PuD1) show great promise as a tool that can facilitate the detection of P. pungens in the phytoplankton samples. The whole-cell FISH method was proven as a rapid detection tool for Pseudonitzschia with high specificity. This approach is suggested to be adopted in the country harmful algae monitoring and mitigation.
format Final Year Project Report
author Suk Hui, Voon
author_facet Suk Hui, Voon
author_sort Suk Hui, Voon
title Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization
title_short Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization
title_full Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization
title_fullStr Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization
title_full_unstemmed Molecular detection of toxic pseudo-nitzschia species (Bacillariophyceae) by whole-cell fluorescene in situ hybridization
title_sort molecular detection of toxic pseudo-nitzschia species (bacillariophyceae) by whole-cell fluorescene in situ hybridization
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2011
url http://ir.unimas.my/id/eprint/5337/8/Voon%20Suk%20Hui.pdf
http://ir.unimas.my/id/eprint/5337/
_version_ 1792160561873551360
score 13.211869

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