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DNA barcoding of sawih (Duabanga moluccana) using ISSR marker

Duabanga moluccana or locally known as Sawih is a widely known forest tree species for its multi-purpose timber and other natural products such as fibers. Genetic diversity investigation of this species can provide baseline information for indirect selection in tree improvement programme. This stud...

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Main Author: Diyanah Musfirah, Bt. Jamal
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2011
Subjects:
SD Forestry
Online Access:http://ir.unimas.my/id/eprint/5306/8/DNA%20barcoding%20of%20SAWIH%20%28Duabanga%20moluccana%29%20using%20ISSR%20marker%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/5306/
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spelling my.unimas.ir.53062023-02-27T04:37:16Z http://ir.unimas.my/id/eprint/5306/ DNA barcoding of sawih (Duabanga moluccana) using ISSR marker Diyanah Musfirah, Bt. Jamal SD Forestry Duabanga moluccana or locally known as Sawih is a widely known forest tree species for its multi-purpose timber and other natural products such as fibers. Genetic diversity investigation of this species can provide baseline information for indirect selection in tree improvement programme. This study has employed Inter Simple Sequence Repeat (ISSR) technique to assess the genetic diversity and relatedness within and between three populations of D. moluccana located in Sarawak, namely, Mukah, Tatau (Bintulu) and Niah. A total of 73 loci from 90 individuals were successfully amplified with three selected ISSR primers and the percentage of polymorphic loci was 100%. The Shannon’s diversity index showed that D. moluccana in Mukah natural forest (0.4993) was the most diverse compared to Tatau (Bintulu) (0.3795) and Niah natural forest (0.3304). Dendrograms were constructed using unweighted pair group method with arithmetic mean (UPGMA) to show relationship within and among the selected populations. The overall populations were mainly clustered into three main groups, according to their corresponding population. Based on these results, it implies that D. moluccana trees are genetically diverse and related both within and among populations. Universiti Malaysia Sarawak, (UNIMAS) 2011 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/5306/8/DNA%20barcoding%20of%20SAWIH%20%28Duabanga%20moluccana%29%20using%20ISSR%20marker%20%28fulltext%29.pdf Diyanah Musfirah, Bt. Jamal (2011) DNA barcoding of sawih (Duabanga moluccana) using ISSR marker. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SD Forestry
spellingShingle SD Forestry
Diyanah Musfirah, Bt. Jamal
DNA barcoding of sawih (Duabanga moluccana) using ISSR marker
description Duabanga moluccana or locally known as Sawih is a widely known forest tree species for its multi-purpose timber and other natural products such as fibers. Genetic diversity investigation of this species can provide baseline information for indirect selection in tree improvement programme. This study has employed Inter Simple Sequence Repeat (ISSR) technique to assess the genetic diversity and relatedness within and between three populations of D. moluccana located in Sarawak, namely, Mukah, Tatau (Bintulu) and Niah. A total of 73 loci from 90 individuals were successfully amplified with three selected ISSR primers and the percentage of polymorphic loci was 100%. The Shannon’s diversity index showed that D. moluccana in Mukah natural forest (0.4993) was the most diverse compared to Tatau (Bintulu) (0.3795) and Niah natural forest (0.3304). Dendrograms were constructed using unweighted pair group method with arithmetic mean (UPGMA) to show relationship within and among the selected populations. The overall populations were mainly clustered into three main groups, according to their corresponding population. Based on these results, it implies that D. moluccana trees are genetically diverse and related both within and among populations.
format Final Year Project Report
author Diyanah Musfirah, Bt. Jamal
author_facet Diyanah Musfirah, Bt. Jamal
author_sort Diyanah Musfirah, Bt. Jamal
title DNA barcoding of sawih (Duabanga moluccana) using ISSR marker
title_short DNA barcoding of sawih (Duabanga moluccana) using ISSR marker
title_full DNA barcoding of sawih (Duabanga moluccana) using ISSR marker
title_fullStr DNA barcoding of sawih (Duabanga moluccana) using ISSR marker
title_full_unstemmed DNA barcoding of sawih (Duabanga moluccana) using ISSR marker
title_sort dna barcoding of sawih (duabanga moluccana) using issr marker
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2011
url http://ir.unimas.my/id/eprint/5306/8/DNA%20barcoding%20of%20SAWIH%20%28Duabanga%20moluccana%29%20using%20ISSR%20marker%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/5306/
_version_ 1758970228718436352
score 13.211869

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