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Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine

A live attenuated vaccine was developed against HVHN of goldfish, caused by cyprinid herpesvirus-2 (CyHV-2). However, the mechanism of immunity acquired after vaccination remains unknown. The objectives of this study are to identify susceptible hosts’ immune responses and investigate the role of ado...

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Main Authors: Hiroaki, Saito, Lau, Lik Ming, Minami, Shungo, Manami, Yuguchi, Aiko, Shitara, Hidehiro, Kondo, Goshi, Kato, Motohiko, Sano
Format: Article
Language:English
Published: Elsevier B.V. 2023
Subjects:
SH Aquaculture. Fisheries. Angling
Online Access:http://ir.unimas.my/id/eprint/45195/1/Adoptive.pdf
http://ir.unimas.my/id/eprint/45195/
https://www.sciencedirect.com/science/article/abs/pii/S0145305X23011540
https://doi.org/10.1016/j.dci.2023.105001
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spelling my.unimas.ir.451952024-07-11T00:33:46Z http://ir.unimas.my/id/eprint/45195/ Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine Hiroaki, Saito Lau, Lik Ming Minami, Shungo Manami, Yuguchi Aiko, Shitara Hidehiro, Kondo Goshi, Kato Motohiko, Sano SH Aquaculture. Fisheries. Angling A live attenuated vaccine was developed against HVHN of goldfish, caused by cyprinid herpesvirus-2 (CyHV-2). However, the mechanism of immunity acquired after vaccination remains unknown. The objectives of this study are to identify susceptible hosts’ immune responses and investigate the role of adoptive transferred sensitized leukocytes in protection against HVHN using the susceptible isogenic ginbuna. Goldfish and ginbuna (Wakin and S3n strain, respectively; BW 8 g) were vaccinated by the showering vaccination method (1:100 diluted vaccine; holding fish on the net for 10 s). Vaccinated fish were challenged with a virulent CyHV-2 by immersion after 21 days and the mortality was recorded. Meanwhile, the trunk-kidney and spleen leukocytes were isolated to determine CD8a+ lymphocyte dynamics. Antibody titer in serum was determined by ELISA using purified virus particles. In the adoptive transfer experiment, pooled whole trunk-kidney and spleen leukocytes of the vaccinated donor ginbuna (n=14) after 55 days were intraperitoneally injected (3.5 x 10^6 cells/fish) into naïve recipient ginbuna (n=10). The serum of donor ginbuna was collected for ELISA. After one day, the donor and recipient ginbuna were challenged with a virulent CyHV-2. The vaccine was effective in both fish species against CyHV-2 challenge considering the increase of CD8a+ lymphocytes and ELISA antibody titer. The adoptive transfer experiment showed survival rate of donor, recipient and infected-control ginbuna at 80%, 40%, and 0%, respectively. Donor ginbuna had low seropositivity before CyHV-2 challenge. These results suggest that humoral and cell-mediated immunity play significant roles in the protective immunity induced by the vaccine. Elsevier B.V. 2023-11 Article NonPeerReviewed text en http://ir.unimas.my/id/eprint/45195/1/Adoptive.pdf Hiroaki, Saito and Lau, Lik Ming and Minami, Shungo and Manami, Yuguchi and Aiko, Shitara and Hidehiro, Kondo and Goshi, Kato and Motohiko, Sano (2023) Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine. Developmental & Comparative Immunology, 148. p. 105001. ISSN 1879-0089 https://www.sciencedirect.com/science/article/abs/pii/S0145305X23011540 https://doi.org/10.1016/j.dci.2023.105001
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SH Aquaculture. Fisheries. Angling
spellingShingle SH Aquaculture. Fisheries. Angling
Hiroaki, Saito
Lau, Lik Ming
Minami, Shungo
Manami, Yuguchi
Aiko, Shitara
Hidehiro, Kondo
Goshi, Kato
Motohiko, Sano
Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine
description A live attenuated vaccine was developed against HVHN of goldfish, caused by cyprinid herpesvirus-2 (CyHV-2). However, the mechanism of immunity acquired after vaccination remains unknown. The objectives of this study are to identify susceptible hosts’ immune responses and investigate the role of adoptive transferred sensitized leukocytes in protection against HVHN using the susceptible isogenic ginbuna. Goldfish and ginbuna (Wakin and S3n strain, respectively; BW 8 g) were vaccinated by the showering vaccination method (1:100 diluted vaccine; holding fish on the net for 10 s). Vaccinated fish were challenged with a virulent CyHV-2 by immersion after 21 days and the mortality was recorded. Meanwhile, the trunk-kidney and spleen leukocytes were isolated to determine CD8a+ lymphocyte dynamics. Antibody titer in serum was determined by ELISA using purified virus particles. In the adoptive transfer experiment, pooled whole trunk-kidney and spleen leukocytes of the vaccinated donor ginbuna (n=14) after 55 days were intraperitoneally injected (3.5 x 10^6 cells/fish) into naïve recipient ginbuna (n=10). The serum of donor ginbuna was collected for ELISA. After one day, the donor and recipient ginbuna were challenged with a virulent CyHV-2. The vaccine was effective in both fish species against CyHV-2 challenge considering the increase of CD8a+ lymphocytes and ELISA antibody titer. The adoptive transfer experiment showed survival rate of donor, recipient and infected-control ginbuna at 80%, 40%, and 0%, respectively. Donor ginbuna had low seropositivity before CyHV-2 challenge. These results suggest that humoral and cell-mediated immunity play significant roles in the protective immunity induced by the vaccine.
format Article
author Hiroaki, Saito
Lau, Lik Ming
Minami, Shungo
Manami, Yuguchi
Aiko, Shitara
Hidehiro, Kondo
Goshi, Kato
Motohiko, Sano
author_facet Hiroaki, Saito
Lau, Lik Ming
Minami, Shungo
Manami, Yuguchi
Aiko, Shitara
Hidehiro, Kondo
Goshi, Kato
Motohiko, Sano
author_sort Hiroaki, Saito
title Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine
title_short Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine
title_full Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine
title_fullStr Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine
title_full_unstemmed Adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (HVHN) in ginbuna crucian carp Carassius auratus langsdorfii immunized with a live attenuated vaccine
title_sort adoptive transfer of sensitized leukocytes confers partial protection against herpesviral hematopoietic necrosis (hvhn) in ginbuna crucian carp carassius auratus langsdorfii immunized with a live attenuated vaccine
publisher Elsevier B.V.
publishDate 2023
url http://ir.unimas.my/id/eprint/45195/1/Adoptive.pdf
http://ir.unimas.my/id/eprint/45195/
https://www.sciencedirect.com/science/article/abs/pii/S0145305X23011540
https://doi.org/10.1016/j.dci.2023.105001
_version_ 1806430296256544768
score 13.211869

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