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Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn

The study of medicinal plants has gained significant interest among researchers because of their potential for therapeutic purposes and the production of natural drugs. In Sarawak, Cassia alata is one of the native plants used for medicinal purposes, such as treatment for constipation, ringworm, a...

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Main Authors: Scholastica Ramih, Bunya, Samuel, Lihan
Format: Article
Language:English
Published: UNIMAS Publisher 2024
Subjects:
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/45169/1/Effects%20of%20Extraction.pdf
http://ir.unimas.my/id/eprint/45169/
https://publisher.unimas.my/ojs/index.php/BJRST/article/view/5701
https:doi.org/10.33736/bjrst.5701.2024
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spelling my.unimas.ir.451692024-07-04T07:46:35Z http://ir.unimas.my/id/eprint/45169/ Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn Scholastica Ramih, Bunya Samuel, Lihan QR Microbiology The study of medicinal plants has gained significant interest among researchers because of their potential for therapeutic purposes and the production of natural drugs. In Sarawak, Cassia alata is one of the native plants used for medicinal purposes, such as treatment for constipation, ringworm, and other skin diseases. This study determined the yield of extraction, total phenolic content (TPC), and total flavonoid content (TFC) of the leaf, stem, and root of C. alata using various extraction methods and solvent extractions. The extractions were performed using soxhlet extraction (SE) and ultrasonic-assisted extraction (UAE) with ethanol and chloroform. Among all, the extract obtained from SE with ethanol solvent (SE-EtOH) showed the highest yield in all plant parts (leaf: 28.62 %, stem: 10.06 %, and root: 9.79 %). Meanwhile, the TPC and TFC estimated using the FolinCiocalteu phenol reagent and aluminium chloride colorimetric assay methods showed that the highest TPC and TFC were from the leaf extract obtained using UAE and chloroform (UAE-Chlo-L) with a TPC value of 117.436 mg GAE/g DW and a TFC value of 568.778 mg QE/g DW, respectively. Overall, the findings demonstrated that chloroform was an effective solvent system for all plant parts on the TPC and TFC, with the leaf part containing the greatest value, and that ultrasonic-assisted extraction was the best approach. This exploration is beneficial for the determination of methods that produce optimum yield, phenolic, and flavonoid content in C. alata’s species. UNIMAS Publisher 2024 Article PeerReviewed text en http://ir.unimas.my/id/eprint/45169/1/Effects%20of%20Extraction.pdf Scholastica Ramih, Bunya and Samuel, Lihan (2024) Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn. Borneo Journal of Resource Science and Technology, 14 (1). pp. 139-148. ISSN 0128-2972 https://publisher.unimas.my/ojs/index.php/BJRST/article/view/5701 https:doi.org/10.33736/bjrst.5701.2024
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Scholastica Ramih, Bunya
Samuel, Lihan
Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn
description The study of medicinal plants has gained significant interest among researchers because of their potential for therapeutic purposes and the production of natural drugs. In Sarawak, Cassia alata is one of the native plants used for medicinal purposes, such as treatment for constipation, ringworm, and other skin diseases. This study determined the yield of extraction, total phenolic content (TPC), and total flavonoid content (TFC) of the leaf, stem, and root of C. alata using various extraction methods and solvent extractions. The extractions were performed using soxhlet extraction (SE) and ultrasonic-assisted extraction (UAE) with ethanol and chloroform. Among all, the extract obtained from SE with ethanol solvent (SE-EtOH) showed the highest yield in all plant parts (leaf: 28.62 %, stem: 10.06 %, and root: 9.79 %). Meanwhile, the TPC and TFC estimated using the FolinCiocalteu phenol reagent and aluminium chloride colorimetric assay methods showed that the highest TPC and TFC were from the leaf extract obtained using UAE and chloroform (UAE-Chlo-L) with a TPC value of 117.436 mg GAE/g DW and a TFC value of 568.778 mg QE/g DW, respectively. Overall, the findings demonstrated that chloroform was an effective solvent system for all plant parts on the TPC and TFC, with the leaf part containing the greatest value, and that ultrasonic-assisted extraction was the best approach. This exploration is beneficial for the determination of methods that produce optimum yield, phenolic, and flavonoid content in C. alata’s species.
format Article
author Scholastica Ramih, Bunya
Samuel, Lihan
author_facet Scholastica Ramih, Bunya
Samuel, Lihan
author_sort Scholastica Ramih, Bunya
title Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn
title_short Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn
title_full Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn
title_fullStr Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn
title_full_unstemmed Effects of Extraction Method on Yield, Phenolic and Flavonoid Content of Leaf, Stem and Root of Cassia alata Linn
title_sort effects of extraction method on yield, phenolic and flavonoid content of leaf, stem and root of cassia alata linn
publisher UNIMAS Publisher
publishDate 2024
url http://ir.unimas.my/id/eprint/45169/1/Effects%20of%20Extraction.pdf
http://ir.unimas.my/id/eprint/45169/
https://publisher.unimas.my/ojs/index.php/BJRST/article/view/5701
https:doi.org/10.33736/bjrst.5701.2024
_version_ 1804070542614986752
score 13.211869

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