Selection of vaccine candidates against Pseudomonas koreensis using reverse vaccinology and a preliminary efficacy trial in Empurau (Tor tambroides)
This study marks the first utilization of reverse vaccinology to develop recombinant subunit vaccines against Pseudomonas koreensis infection in Empurau (Tor tambroides). The proteome (5538 proteins) was screened against various filters to prioritize proteins based on features that are associated...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier B.V.
2024
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/44958/2/Selection%20of%20vaccine%20candidates%20-%20Copy.pdf http://ir.unimas.my/id/eprint/44958/ https://www.sciencedirect.com/science/article/abs/pii/S1050464824003334 https://doi.org/10.1016/j.fsi.2024.109688 |
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| Summary: | This study marks the first utilization of reverse vaccinology to develop recombinant subunit vaccines against
Pseudomonas koreensis infection in Empurau (Tor tambroides). The proteome (5538 proteins) was screened against
various filters to prioritize proteins based on features that are associated with virulence, subcellular localization, transmembrane helical structure, antigenicity, essentiality, non-homology with the host proteome, molecular
weight, and stability, which led to the identification of eight potential vaccine candidates. These potential
vaccine candidates were cloned and expressed, with six achieving successful expression and purification. The
antigens were formulated into two distinct vaccine mixtures, Vac A and Vac B, and their protective efficacy was
assessed through in vivo challenge experiments. Vac A and Vac B demonstrated high protective efficacies of 100
% and 81.2 %, respectively. Histological analyses revealed reduced tissue damage in vaccinated fish after
experimental infection, with Vac A showing no adverse effects, whereas Vac B exhibited mild degenerative
changes. Quantitative real-time PCR results showed a significant upregulation of TNF-α and downregulation of
IL-1β in the kidneys, spleen, gills, and intestine in both Vac A- and Vac B-immunized fish after challenged with
P. koreensis. Additionally, IL-8 exhibits tissue-specific differential expression, with significant upregulation in the kidney, gills, and intestine, and downregulation in the spleen, particularly notable in Vac A-immunized fish. The
research underscores the effectiveness of the reverse vaccinology approach in fish and demonstrates the promising potential of Vac A and Vac B as recombinant subunit vaccines. |
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