Cover Image

Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection

Approximately 600 million people worldwide are infected with Strongyloides stercoralis. Many diagnostic laboratories use serology to detect the infection. SsRapidVR is a prototype IgG4 lateral flow test based on NIE recombinant protein. We compared SsRapid with two commercial IgG-ELISAs (Bordier a...

Full description

Saved in:
Bibliographic Details
Main Authors: Nor Suhada, Anuar, Anizah, Rahumatullah, Norashikin, Samsudin, Zeehaida, Mohamed, Emelia, Osman, Nik Zairi, Zakaria, Hussain, Ahmad, Rahmah, Noordin
Format: Article
Language:English
Published: The American Society of Tropical Medicine and Hygiene 2023
Subjects:
QR Microbiology
R Medicine (General)
Online Access:http://ir.unimas.my/id/eprint/44879/1/Performance%20Assessment.pdf
http://ir.unimas.my/id/eprint/44879/
https://www.ajtmh.org/view/journals/tpmd/108/2/article-p353.xml?rskey=LnqCQF&result=1
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.44879
record_format eprints
spelling my.unimas.ir.448792024-06-13T03:06:29Z http://ir.unimas.my/id/eprint/44879/ Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection Nor Suhada, Anuar Anizah, Rahumatullah Norashikin, Samsudin Zeehaida, Mohamed Emelia, Osman Nik Zairi, Zakaria Hussain, Ahmad Rahmah, Noordin QR Microbiology R Medicine (General) Approximately 600 million people worldwide are infected with Strongyloides stercoralis. Many diagnostic laboratories use serology to detect the infection. SsRapidVR is a prototype IgG4 lateral flow test based on NIE recombinant protein. We compared SsRapid with two commercial IgG-ELISAs (Bordier and Euroimmun) using five sera groups (G1–5, N 5 170). Healthy individuals from nonendemic areas (G1, N 5 33) and Strongyloides larvae-positive patients (G2, N 5 20) showed no significant difference (P . 0.05) among the three assays. The group of other parasitic infections (G3) showed that SsRapid results were more concordant with Bordier ELISA (73%, 35/48) than Euroimmun ELISA (65%, 31/48). In corticosteroid-treated cancer patients who were polymerase chain reaction–positive for Strongyloides (G4, N 5 25), SsRapid showed a higher detection rate (28%, 7/25) than both ELISAs (4%, 1/25). Meanwhile, SsRapid showed significantly less infection prevalence among the endemic area population (G5) than the ELISAs. Overall, SsRapid showed good performance in detecting Strongyloides infection compared with the commercial IgG-ELISAs. The American Society of Tropical Medicine and Hygiene 2023 Article PeerReviewed text en http://ir.unimas.my/id/eprint/44879/1/Performance%20Assessment.pdf Nor Suhada, Anuar and Anizah, Rahumatullah and Norashikin, Samsudin and Zeehaida, Mohamed and Emelia, Osman and Nik Zairi, Zakaria and Hussain, Ahmad and Rahmah, Noordin (2023) Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection. The American Journal of Tropical Medicine and Hygiene, 108 (2). pp. 353-355. ISSN 1476-1645 https://www.ajtmh.org/view/journals/tpmd/108/2/article-p353.xml?rskey=LnqCQF&result=1 doi:10.4269/ajtmh.22-0592
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
R Medicine (General)
spellingShingle QR Microbiology
R Medicine (General)
Nor Suhada, Anuar
Anizah, Rahumatullah
Norashikin, Samsudin
Zeehaida, Mohamed
Emelia, Osman
Nik Zairi, Zakaria
Hussain, Ahmad
Rahmah, Noordin
Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection
description Approximately 600 million people worldwide are infected with Strongyloides stercoralis. Many diagnostic laboratories use serology to detect the infection. SsRapidVR is a prototype IgG4 lateral flow test based on NIE recombinant protein. We compared SsRapid with two commercial IgG-ELISAs (Bordier and Euroimmun) using five sera groups (G1–5, N 5 170). Healthy individuals from nonendemic areas (G1, N 5 33) and Strongyloides larvae-positive patients (G2, N 5 20) showed no significant difference (P . 0.05) among the three assays. The group of other parasitic infections (G3) showed that SsRapid results were more concordant with Bordier ELISA (73%, 35/48) than Euroimmun ELISA (65%, 31/48). In corticosteroid-treated cancer patients who were polymerase chain reaction–positive for Strongyloides (G4, N 5 25), SsRapid showed a higher detection rate (28%, 7/25) than both ELISAs (4%, 1/25). Meanwhile, SsRapid showed significantly less infection prevalence among the endemic area population (G5) than the ELISAs. Overall, SsRapid showed good performance in detecting Strongyloides infection compared with the commercial IgG-ELISAs.
format Article
author Nor Suhada, Anuar
Anizah, Rahumatullah
Norashikin, Samsudin
Zeehaida, Mohamed
Emelia, Osman
Nik Zairi, Zakaria
Hussain, Ahmad
Rahmah, Noordin
author_facet Nor Suhada, Anuar
Anizah, Rahumatullah
Norashikin, Samsudin
Zeehaida, Mohamed
Emelia, Osman
Nik Zairi, Zakaria
Hussain, Ahmad
Rahmah, Noordin
author_sort Nor Suhada, Anuar
title Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection
title_short Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection
title_full Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection
title_fullStr Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection
title_full_unstemmed Performance Assessment of a Lateral Flow Rapid Test (SsRapidVR) Compared with Two Commercial ELISAs in Detecting Strongyloides Infection
title_sort performance assessment of a lateral flow rapid test (ssrapidvr) compared with two commercial elisas in detecting strongyloides infection
publisher The American Society of Tropical Medicine and Hygiene
publishDate 2023
url http://ir.unimas.my/id/eprint/44879/1/Performance%20Assessment.pdf
http://ir.unimas.my/id/eprint/44879/
https://www.ajtmh.org/view/journals/tpmd/108/2/article-p353.xml?rskey=LnqCQF&result=1
_version_ 1802981815586127872
score 13.211869

Similar Items