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Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling

The gut microbiome refers to the microorganism community living within the digestive tract. The environment plays a crucial role in shaping the gut microbiome composition of animals. The gut microbiome influences the health and behavior of animals, including the critically endangered Malayan tiger (...

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Main Authors: Millawati, Gani, Mohd Ridwan, Abd Rahman, Frankie Thomas, Sitam, ZUBAIDAH, KAMARUDIN, SITI SUZANA, SELAMAT, Nik Mohd Zamani, Awang, Kayal Vizi, Karuppannan, Badrul Munir, Md Zain
Format: Article
Language:English
Published: Springer Nature 2024
Subjects:
QL Zoology
Online Access:http://ir.unimas.my/id/eprint/44418/1/s11274-023-03868-x
http://ir.unimas.my/id/eprint/44418/
https://link.springer.com/article/10.1007/s11274-023-03868-x
https://doi.org/10.1007/s11274-023-03868-x
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spelling my.unimas.ir.444182024-03-05T03:25:32Z http://ir.unimas.my/id/eprint/44418/ Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling Millawati, Gani Mohd Ridwan, Abd Rahman Frankie Thomas, Sitam ZUBAIDAH, KAMARUDIN SITI SUZANA, SELAMAT Nik Mohd Zamani, Awang Kayal Vizi, Karuppannan Badrul Munir, Md Zain QL Zoology The gut microbiome refers to the microorganism community living within the digestive tract. The environment plays a crucial role in shaping the gut microbiome composition of animals. The gut microbiome influences the health and behavior of animals, including the critically endangered Malayan tiger (Panthera tigris jacksoni). However, the gut microbiome composition of Malayan tigers, especially those living in their natural habitats, remains poorly understood. To address this knowledge gap, we used next-generation sequencing DNA metabarcoding techniques to analyze the gut microbiome of wild Malayan tigers using fecal samples collected from their natural habitats and in captivity. Our aim was to determine the gut microbiota composition of the Malayan tiger, considering the different types of habitat environments. The results revealed a diverse microbial community within the gut microbiome of Malayan tigers. The prominent phyla that were observed included Firmicutes, Proteobacteria, Actinobacteriota, Fusobacteriota and Bacteroidota. Beta diversity analysis revealed significant differences in gut microbiome composition of Malayan tigers that inhabited oil palm plantations, in villages and protected areas. Diversity analysis also revealed significant difference in the gut microbiome between wild and captive Malayan tigers. However, the distinctions of gut microbiome between wild and captive alpha diversity did not yield significant differences. The differences in microbiome diversity resulted from the interplay of dietary intake and environmental factors. This information will facilitate the establishment of focused conservation approaches and enhance our understanding of the effect of microbiome composition on Malayan tiger health. Springer Nature 2024-02 Article PeerReviewed text en http://ir.unimas.my/id/eprint/44418/1/s11274-023-03868-x Millawati, Gani and Mohd Ridwan, Abd Rahman and Frankie Thomas, Sitam and ZUBAIDAH, KAMARUDIN and SITI SUZANA, SELAMAT and Nik Mohd Zamani, Awang and Kayal Vizi, Karuppannan and Badrul Munir, Md Zain (2024) Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling. World Journal of Microbiology and Biotechnology, 40 (111). ISSN 1573-0972 https://link.springer.com/article/10.1007/s11274-023-03868-x https://doi.org/10.1007/s11274-023-03868-x
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QL Zoology
spellingShingle QL Zoology
Millawati, Gani
Mohd Ridwan, Abd Rahman
Frankie Thomas, Sitam
ZUBAIDAH, KAMARUDIN
SITI SUZANA, SELAMAT
Nik Mohd Zamani, Awang
Kayal Vizi, Karuppannan
Badrul Munir, Md Zain
Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling
description The gut microbiome refers to the microorganism community living within the digestive tract. The environment plays a crucial role in shaping the gut microbiome composition of animals. The gut microbiome influences the health and behavior of animals, including the critically endangered Malayan tiger (Panthera tigris jacksoni). However, the gut microbiome composition of Malayan tigers, especially those living in their natural habitats, remains poorly understood. To address this knowledge gap, we used next-generation sequencing DNA metabarcoding techniques to analyze the gut microbiome of wild Malayan tigers using fecal samples collected from their natural habitats and in captivity. Our aim was to determine the gut microbiota composition of the Malayan tiger, considering the different types of habitat environments. The results revealed a diverse microbial community within the gut microbiome of Malayan tigers. The prominent phyla that were observed included Firmicutes, Proteobacteria, Actinobacteriota, Fusobacteriota and Bacteroidota. Beta diversity analysis revealed significant differences in gut microbiome composition of Malayan tigers that inhabited oil palm plantations, in villages and protected areas. Diversity analysis also revealed significant difference in the gut microbiome between wild and captive Malayan tigers. However, the distinctions of gut microbiome between wild and captive alpha diversity did not yield significant differences. The differences in microbiome diversity resulted from the interplay of dietary intake and environmental factors. This information will facilitate the establishment of focused conservation approaches and enhance our understanding of the effect of microbiome composition on Malayan tiger health.
format Article
author Millawati, Gani
Mohd Ridwan, Abd Rahman
Frankie Thomas, Sitam
ZUBAIDAH, KAMARUDIN
SITI SUZANA, SELAMAT
Nik Mohd Zamani, Awang
Kayal Vizi, Karuppannan
Badrul Munir, Md Zain
author_facet Millawati, Gani
Mohd Ridwan, Abd Rahman
Frankie Thomas, Sitam
ZUBAIDAH, KAMARUDIN
SITI SUZANA, SELAMAT
Nik Mohd Zamani, Awang
Kayal Vizi, Karuppannan
Badrul Munir, Md Zain
author_sort Millawati, Gani
title Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling
title_short Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling
title_full Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling
title_fullStr Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling
title_full_unstemmed Habitat shapes the gut microbiome diversity of Malayan tigers (Panthera tigris jacksoni) as revealed through metabarcoding 16S rRNA profiling
title_sort habitat shapes the gut microbiome diversity of malayan tigers (panthera tigris jacksoni) as revealed through metabarcoding 16s rrna profiling
publisher Springer Nature
publishDate 2024
url http://ir.unimas.my/id/eprint/44418/1/s11274-023-03868-x
http://ir.unimas.my/id/eprint/44418/
https://link.springer.com/article/10.1007/s11274-023-03868-x
https://doi.org/10.1007/s11274-023-03868-x
_version_ 1793163080468791296
score 13.211869

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