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Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak

Neolamarckia cadamba or commonly known as kelampayan has been selected as one of the important plantation tree species in Malaysia. Thus, the molecular characterization of this indigenous tropical tree species is needed to maintain its high quality. Inter simple sequence repeats (ISSR) markers wer...

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Main Authors: Tiong, S.Y, Chew, S.F, Ho, W.S, Pang, S.L
Format: E-Article
Language:English
Published: Pelagia Research Library 2014
Subjects:
SB Plant culture
SD Forestry
Online Access:http://ir.unimas.my/id/eprint/4427/1/Genetic%20diversity%20of%20Neolamarckia%20cadamba%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/4427/
http://www.pelagiaresearchlibrary.com
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spelling my.unimas.ir.44272015-09-14T02:52:18Z http://ir.unimas.my/id/eprint/4427/ Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak Tiong, S.Y Chew, S.F Ho, W.S Pang, S.L SB Plant culture SD Forestry Neolamarckia cadamba or commonly known as kelampayan has been selected as one of the important plantation tree species in Malaysia. Thus, the molecular characterization of this indigenous tropical tree species is needed to maintain its high quality. Inter simple sequence repeats (ISSR) markers were used in this study to determine the genetic diversity and relatedness of N. cadamba in two planted forests and six natural forests in Sarawak. Three ISSR primers had generated atotal of 239 loci, of which 32.6% - 59.4% of the loci were polymorphic among236N. cadamba treesin eight populations. The mean Shannon’s diversity index (I)ranged from 0.1399 to 0.2354. The coefficient of population differentiation was low for planted forests (Gst = 0.0871) and natural forests (Gst = 0.2013`). Both UPGMA dendrogram and NJ-tree generated by ISSR markers had divided natural forests and planted forests into two distinct clusters. Natural forests were grouped in one cluster while planted forests were grouped in another cluster. This study shows that N. cadamba trees are closely related within its own population and its designated forest type. In future, several specific loci can be sequenced and developed into SCAR (sequence characterized amplification region) markers for tree improvement and conservation programme of N. cadamba. Pelagia Research Library 2014 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/4427/1/Genetic%20diversity%20of%20Neolamarckia%20cadamba%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf Tiong, S.Y and Chew, S.F and Ho, W.S and Pang, S.L (2014) Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak. Advances in Applied Science Research, 5 (3). pp. 458-463. ISSN 0976-8610 http://www.pelagiaresearchlibrary.com
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic SB Plant culture
SD Forestry
spellingShingle SB Plant culture
SD Forestry
Tiong, S.Y
Chew, S.F
Ho, W.S
Pang, S.L
Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
description Neolamarckia cadamba or commonly known as kelampayan has been selected as one of the important plantation tree species in Malaysia. Thus, the molecular characterization of this indigenous tropical tree species is needed to maintain its high quality. Inter simple sequence repeats (ISSR) markers were used in this study to determine the genetic diversity and relatedness of N. cadamba in two planted forests and six natural forests in Sarawak. Three ISSR primers had generated atotal of 239 loci, of which 32.6% - 59.4% of the loci were polymorphic among236N. cadamba treesin eight populations. The mean Shannon’s diversity index (I)ranged from 0.1399 to 0.2354. The coefficient of population differentiation was low for planted forests (Gst = 0.0871) and natural forests (Gst = 0.2013`). Both UPGMA dendrogram and NJ-tree generated by ISSR markers had divided natural forests and planted forests into two distinct clusters. Natural forests were grouped in one cluster while planted forests were grouped in another cluster. This study shows that N. cadamba trees are closely related within its own population and its designated forest type. In future, several specific loci can be sequenced and developed into SCAR (sequence characterized amplification region) markers for tree improvement and conservation programme of N. cadamba.
format E-Article
author Tiong, S.Y
Chew, S.F
Ho, W.S
Pang, S.L
author_facet Tiong, S.Y
Chew, S.F
Ho, W.S
Pang, S.L
author_sort Tiong, S.Y
title Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_short Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_full Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_fullStr Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_full_unstemmed Genetic diversity of Neolamarckia cadamba using dominant DNA markers based on inter-simple sequence repeats (ISSRs) in Sarawak
title_sort genetic diversity of neolamarckia cadamba using dominant dna markers based on inter-simple sequence repeats (issrs) in sarawak
publisher Pelagia Research Library
publishDate 2014
url http://ir.unimas.my/id/eprint/4427/1/Genetic%20diversity%20of%20Neolamarckia%20cadamba%20using%20dominant%20DNA%20markers%20based%20on%20inter-simple%20sequence%20repeats%20%28ISSRs%29%20in%20Sarawak%20%28abstract%29.pdf
http://ir.unimas.my/id/eprint/4427/
http://www.pelagiaresearchlibrary.com
_version_ 1644509555639451648
score 13.211869

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