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Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses

Enteroviruses (EVs) are a group of viruses that cause various human illnesses. While the CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) method can generate VP1 gene fragments for enterovirus genotyping, it is unable to detect recombinant strains. Recent advances in viral genome sequ...

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Main Authors: Yeh-Sheng, Chien, Feng-Jui, Chen, Han-Chieh, Wu, Chieh-Hua, Lin, Wen-Chiung, Chang, David, Perera, Jyh-Yuan, Yang, Min-Shi, Lee, Yu-Chieh, Liao
Format: Article
Language:English
Published: AMER SOC MICROBIOLOGY 2023
Subjects:
R Medicine (General)
RZ Other systems of medicine
Online Access:http://ir.unimas.my/id/eprint/43971/1/chien-et-al-2023-cost-effective-complete-genome-sequencing-using-the-minion-platform-for-identification-of-recombinant-1-2.pdf
http://ir.unimas.my/id/eprint/43971/
https://journals.asm.org/doi/10.1128/spectrum.02507-23
https://doi.org/10.1128/spectrum.02507-23
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spelling my.unimas.ir.439712024-01-02T02:16:02Z http://ir.unimas.my/id/eprint/43971/ Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses Yeh-Sheng, Chien Feng-Jui, Chen Han-Chieh, Wu Chieh-Hua, Lin Wen-Chiung, Chang David, Perera Jyh-Yuan, Yang Min-Shi, Lee Yu-Chieh, Liao R Medicine (General) RZ Other systems of medicine Enteroviruses (EVs) are a group of viruses that cause various human illnesses. While the CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) method can generate VP1 gene fragments for enterovirus genotyping, it is unable to detect recombinant strains. Recent advances in viral genome sequencing using next-generation sequencing technologies have enabled comprehensive analyses. However, the high cost poses a challenge for widespread adoption. To address this issue, this study proposes a cost-effective approach for generating complete enterovirus genome sequences using the Oxford Nanopore MinION sequencer. This protocol not only facilitates the generation of accurate genome sequences for various enterovirus strains but also allows for the differentiation of co-infections from viral isolates. In addition, the method can generate polyprotein sequences as well as peptide sequences of the upstream ORF (uORF) whose expression can impact virus infection. Through the analysis of complete enterovirus genomes, this study successfully identified seven enterovirus A71 isolates obtained during the 2018 enterovirus outbreak in Malaysia and Taiwan as recombinants between enterovirus A71 and coxsackievirus A2. Furthermore, our study has made a significant discovery by establishing a strong correlation between uORF trees and the epidemics of EVA71. This finding highlights the potential of uORF sequences as valuable indicators for monitoring and understanding the spread of EVA71 infections. We also identified notable amino acid changes in the transmembrane domain of the uORF protein within a newly identified lineage. These findings provide crucial insights into the molecular characteristics and evolutionary dynamics of EVA71, offering valuable information for future research and intervention strategies. AMER SOC MICROBIOLOGY 2023-10-13 Article PeerReviewed text en http://ir.unimas.my/id/eprint/43971/1/chien-et-al-2023-cost-effective-complete-genome-sequencing-using-the-minion-platform-for-identification-of-recombinant-1-2.pdf Yeh-Sheng, Chien and Feng-Jui, Chen and Han-Chieh, Wu and Chieh-Hua, Lin and Wen-Chiung, Chang and David, Perera and Jyh-Yuan, Yang and Min-Shi, Lee and Yu-Chieh, Liao (2023) Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses. Microbiology Spectrum, 11 (6). pp. 1-15. ISSN 2165-0497 https://journals.asm.org/doi/10.1128/spectrum.02507-23 https://doi.org/10.1128/spectrum.02507-23
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic R Medicine (General)
RZ Other systems of medicine
spellingShingle R Medicine (General)
RZ Other systems of medicine
Yeh-Sheng, Chien
Feng-Jui, Chen
Han-Chieh, Wu
Chieh-Hua, Lin
Wen-Chiung, Chang
David, Perera
Jyh-Yuan, Yang
Min-Shi, Lee
Yu-Chieh, Liao
Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses
description Enteroviruses (EVs) are a group of viruses that cause various human illnesses. While the CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) method can generate VP1 gene fragments for enterovirus genotyping, it is unable to detect recombinant strains. Recent advances in viral genome sequencing using next-generation sequencing technologies have enabled comprehensive analyses. However, the high cost poses a challenge for widespread adoption. To address this issue, this study proposes a cost-effective approach for generating complete enterovirus genome sequences using the Oxford Nanopore MinION sequencer. This protocol not only facilitates the generation of accurate genome sequences for various enterovirus strains but also allows for the differentiation of co-infections from viral isolates. In addition, the method can generate polyprotein sequences as well as peptide sequences of the upstream ORF (uORF) whose expression can impact virus infection. Through the analysis of complete enterovirus genomes, this study successfully identified seven enterovirus A71 isolates obtained during the 2018 enterovirus outbreak in Malaysia and Taiwan as recombinants between enterovirus A71 and coxsackievirus A2. Furthermore, our study has made a significant discovery by establishing a strong correlation between uORF trees and the epidemics of EVA71. This finding highlights the potential of uORF sequences as valuable indicators for monitoring and understanding the spread of EVA71 infections. We also identified notable amino acid changes in the transmembrane domain of the uORF protein within a newly identified lineage. These findings provide crucial insights into the molecular characteristics and evolutionary dynamics of EVA71, offering valuable information for future research and intervention strategies.
format Article
author Yeh-Sheng, Chien
Feng-Jui, Chen
Han-Chieh, Wu
Chieh-Hua, Lin
Wen-Chiung, Chang
David, Perera
Jyh-Yuan, Yang
Min-Shi, Lee
Yu-Chieh, Liao
author_facet Yeh-Sheng, Chien
Feng-Jui, Chen
Han-Chieh, Wu
Chieh-Hua, Lin
Wen-Chiung, Chang
David, Perera
Jyh-Yuan, Yang
Min-Shi, Lee
Yu-Chieh, Liao
author_sort Yeh-Sheng, Chien
title Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses
title_short Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses
title_full Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses
title_fullStr Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses
title_full_unstemmed Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses
title_sort cost-effective complete genome sequencing using the minion platform for identification of recombinant enteroviruses
publisher AMER SOC MICROBIOLOGY
publishDate 2023
url http://ir.unimas.my/id/eprint/43971/1/chien-et-al-2023-cost-effective-complete-genome-sequencing-using-the-minion-platform-for-identification-of-recombinant-1-2.pdf
http://ir.unimas.my/id/eprint/43971/
https://journals.asm.org/doi/10.1128/spectrum.02507-23
https://doi.org/10.1128/spectrum.02507-23
_version_ 1787140559466397696
score 13.211869

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