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Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane

1. Diabetic chronic wounds, mainly foot ulcers, constitute one of the most common complications of poorly managed diabetes mellitus. The most typical reasons are insufficient glycemic management, latent neuropathy, peripheral vascular disease, and neglected foot care. In addition, it is a common ca...

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Main Authors: Abbirami, Balachandran, Sy, Bing Choi, Beata, Morak Młodawsk, Jelen, Małgorzata, Gabriele Ruth Anisah, Froemming, Charlie A., Lavilla, Merell P., Billacura, Stepfanie N., Siyumbwa, Patrick N., Okechukwu
Format: Article
Language:English
Published: MDPI 2023
Subjects:
QD Chemistry
R Medicine (General)
RB Pathology
Online Access:http://ir.unimas.my/id/eprint/41791/1/Antioxidant%2C%20Wound.pdf
http://ir.unimas.my/id/eprint/41791/
https://www.mdpi.com/1420-3049/28/3/1043
https://doi.org/10.3390/molecules28031043
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spelling my.unimas.ir.417912023-05-09T07:37:20Z http://ir.unimas.my/id/eprint/41791/ Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane Abbirami, Balachandran Sy, Bing Choi Beata, Morak Młodawsk Jelen, Małgorzata Gabriele Ruth Anisah, Froemming Charlie A., Lavilla Merell P., Billacura Stepfanie N., Siyumbwa Patrick N., Okechukwu QD Chemistry R Medicine (General) RB Pathology 1. Diabetic chronic wounds, mainly foot ulcers, constitute one of the most common complications of poorly managed diabetes mellitus. The most typical reasons are insufficient glycemic management, latent neuropathy, peripheral vascular disease, and neglected foot care. In addition, it is a common cause of foot osteomyelitis and amputation of the lower extremities. Patients are admitted in larger numbers attributable to chronic wounds compared to any other diabetic disease. In the United States, diabetes is currently the most common cause of non-traumatic amputations. Approximately five percent of diabetics develop foot ulcers, and one percent require amputation. Therefore, it is necessary to identify sources of lead with wound-healing properties. Redox imbalance due to excessive oxidative stress is one of the causes for the development of diabetic wounds. Antioxidants have been shown to decrease the progression of diabetic neuropathy by scavenging ROS, regenerating endogenous and exogenous antioxidants, and reversing redox imbalance. Matrix metalloproteinases (MMPs) play vital roles in numerous phases of the wound healing process. Antioxidant and fibroblast cell migration activity of Marantodes pumilum (MP) crude extract has previously been reported. Through their antioxidant, epithelialization, collagen synthesis, and fibroblast migration activities, the authors hypothesise that naringin, eicosane and octacosane identified in the MP extract may have wound-healing properties. 2. The present study aims to identify the bioactive components present in the dichloromethane (DCM) extract of M. pumilum and evaluate their antioxidant and wound healing activity. Bioactive components were identified using LCMS, HPTLC and GCMS. Excision wound on STZ-induced diabetic rat model, human dermal fibroblast (HDF) cell line and colorimetric antioxidant assays were used to evaluate wound healing and antioxidant activities, respectively. Molecular docking and pkCMS software would be utilised to predict binding energy and affinity, as well as ADME parameters. 3. Naringin (NAR), eicosane (EIC), and octacosane (OCT) present in MP displayed antioxidant action and wound excision closure. Histological examination HDF cell line demonstrates epithelialization, collagen production, fibroblast migration, polymorphonuclear leukocyte migration (PNML), and fibroblast movement. The results of molecular docking indicate a substantial attraction and contact between MMPs. pkCMS prediction indicates inadequate blood-brain barrier permeability, low toxicity, and absence of hepatotoxicity. 4. Wound healing properties of (NEO) naringin, eicosane and octacosane may be the result of their antioxidant properties and possible interactions with MMP. MDPI 2023 Article PeerReviewed text en http://ir.unimas.my/id/eprint/41791/1/Antioxidant%2C%20Wound.pdf Abbirami, Balachandran and Sy, Bing Choi and Beata, Morak Młodawsk and Jelen, Małgorzata and Gabriele Ruth Anisah, Froemming and Charlie A., Lavilla and Merell P., Billacura and Stepfanie N., Siyumbwa and Patrick N., Okechukwu (2023) Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane. Molecules, 28 (3). pp. 1-30. ISSN 1420-3049 https://www.mdpi.com/1420-3049/28/3/1043 https://doi.org/10.3390/molecules28031043
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QD Chemistry
R Medicine (General)
RB Pathology
spellingShingle QD Chemistry
R Medicine (General)
RB Pathology
Abbirami, Balachandran
Sy, Bing Choi
Beata, Morak Młodawsk
Jelen, Małgorzata
Gabriele Ruth Anisah, Froemming
Charlie A., Lavilla
Merell P., Billacura
Stepfanie N., Siyumbwa
Patrick N., Okechukwu
Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane
description 1. Diabetic chronic wounds, mainly foot ulcers, constitute one of the most common complications of poorly managed diabetes mellitus. The most typical reasons are insufficient glycemic management, latent neuropathy, peripheral vascular disease, and neglected foot care. In addition, it is a common cause of foot osteomyelitis and amputation of the lower extremities. Patients are admitted in larger numbers attributable to chronic wounds compared to any other diabetic disease. In the United States, diabetes is currently the most common cause of non-traumatic amputations. Approximately five percent of diabetics develop foot ulcers, and one percent require amputation. Therefore, it is necessary to identify sources of lead with wound-healing properties. Redox imbalance due to excessive oxidative stress is one of the causes for the development of diabetic wounds. Antioxidants have been shown to decrease the progression of diabetic neuropathy by scavenging ROS, regenerating endogenous and exogenous antioxidants, and reversing redox imbalance. Matrix metalloproteinases (MMPs) play vital roles in numerous phases of the wound healing process. Antioxidant and fibroblast cell migration activity of Marantodes pumilum (MP) crude extract has previously been reported. Through their antioxidant, epithelialization, collagen synthesis, and fibroblast migration activities, the authors hypothesise that naringin, eicosane and octacosane identified in the MP extract may have wound-healing properties. 2. The present study aims to identify the bioactive components present in the dichloromethane (DCM) extract of M. pumilum and evaluate their antioxidant and wound healing activity. Bioactive components were identified using LCMS, HPTLC and GCMS. Excision wound on STZ-induced diabetic rat model, human dermal fibroblast (HDF) cell line and colorimetric antioxidant assays were used to evaluate wound healing and antioxidant activities, respectively. Molecular docking and pkCMS software would be utilised to predict binding energy and affinity, as well as ADME parameters. 3. Naringin (NAR), eicosane (EIC), and octacosane (OCT) present in MP displayed antioxidant action and wound excision closure. Histological examination HDF cell line demonstrates epithelialization, collagen production, fibroblast migration, polymorphonuclear leukocyte migration (PNML), and fibroblast movement. The results of molecular docking indicate a substantial attraction and contact between MMPs. pkCMS prediction indicates inadequate blood-brain barrier permeability, low toxicity, and absence of hepatotoxicity. 4. Wound healing properties of (NEO) naringin, eicosane and octacosane may be the result of their antioxidant properties and possible interactions with MMP.
format Article
author Abbirami, Balachandran
Sy, Bing Choi
Beata, Morak Młodawsk
Jelen, Małgorzata
Gabriele Ruth Anisah, Froemming
Charlie A., Lavilla
Merell P., Billacura
Stepfanie N., Siyumbwa
Patrick N., Okechukwu
author_facet Abbirami, Balachandran
Sy, Bing Choi
Beata, Morak Młodawsk
Jelen, Małgorzata
Gabriele Ruth Anisah, Froemming
Charlie A., Lavilla
Merell P., Billacura
Stepfanie N., Siyumbwa
Patrick N., Okechukwu
author_sort Abbirami, Balachandran
title Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane
title_short Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane
title_full Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane
title_fullStr Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane
title_full_unstemmed Antioxidant, Wound Healing Potential and In Silico Assessment of Naringin, Eicosane and Octacosane
title_sort antioxidant, wound healing potential and in silico assessment of naringin, eicosane and octacosane
publisher MDPI
publishDate 2023
url http://ir.unimas.my/id/eprint/41791/1/Antioxidant%2C%20Wound.pdf
http://ir.unimas.my/id/eprint/41791/
https://www.mdpi.com/1420-3049/28/3/1043
https://doi.org/10.3390/molecules28031043
_version_ 1767209874076205056
score 13.211869

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