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In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts

Clinacanthus nutans (CN), from the Acanthaceae family is a medicinal plant widely used in Thailand and Malaysia. CN is commonly used as a treatment of inflammation, cancer, and herpes virus infection. This study aimed to determine the antioxidant activity and anticancer properties of CN leaves extra...

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Main Authors: Fong, Isabel Lim, Phung, Joyce Hui Yie, Khong, Heng Yen, Ban, Weng Kit
Format: Article
Language:English
Published: Science, Engineering and Health Studies (SEHS) 2021
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/41540/1/Isabel%20Fong%20Lim.pdf
http://ir.unimas.my/id/eprint/41540/
https://li01.tci-thaijo.org/index.php/sehs/article/view/248287
https://doi.org/10.14456/sehs.2021.51
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spelling my.unimas.ir.415402023-03-16T08:26:49Z http://ir.unimas.my/id/eprint/41540/ In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts Fong, Isabel Lim Phung, Joyce Hui Yie Khong, Heng Yen Ban, Weng Kit Q Science (General) Clinacanthus nutans (CN), from the Acanthaceae family is a medicinal plant widely used in Thailand and Malaysia. CN is commonly used as a treatment of inflammation, cancer, and herpes virus infection. This study aimed to determine the antioxidant activity and anticancer properties of CN leaves extracts on human colorectal cancer cell lines, HCT 116 and HT-29. In this study, CN leaves powders were extracted in methanol, chloroform, and acetone at different durations. The crude extracts were assessed for total phenolic content (TPC), total flavonoid contents (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay. The extract using acetone showed with the highest TPC. The highest total flavonoid content was the methanol extract. The DPPH radical scavenging activity, IC50 of methanol extract was 19.67 µg/mL and exhibited the most efficacious antioxidant property among the others. The 24-hour methanol extract showed the most promising results on MTT assay. Therefore, CN methanol extract is a promising candidate to proceed in other anticancer studies such as cell cycle arrest and apoptosis assays. Science, Engineering and Health Studies (SEHS) 2021-12-27 Article PeerReviewed text en http://ir.unimas.my/id/eprint/41540/1/Isabel%20Fong%20Lim.pdf Fong, Isabel Lim and Phung, Joyce Hui Yie and Khong, Heng Yen and Ban, Weng Kit (2021) In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts. Science, Engineering and Health Studies, 15. pp. 1-6. ISSN 2630 - 0087 (Online) https://li01.tci-thaijo.org/index.php/sehs/article/view/248287 https://doi.org/10.14456/sehs.2021.51
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Fong, Isabel Lim
Phung, Joyce Hui Yie
Khong, Heng Yen
Ban, Weng Kit
In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts
description Clinacanthus nutans (CN), from the Acanthaceae family is a medicinal plant widely used in Thailand and Malaysia. CN is commonly used as a treatment of inflammation, cancer, and herpes virus infection. This study aimed to determine the antioxidant activity and anticancer properties of CN leaves extracts on human colorectal cancer cell lines, HCT 116 and HT-29. In this study, CN leaves powders were extracted in methanol, chloroform, and acetone at different durations. The crude extracts were assessed for total phenolic content (TPC), total flavonoid contents (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay. The extract using acetone showed with the highest TPC. The highest total flavonoid content was the methanol extract. The DPPH radical scavenging activity, IC50 of methanol extract was 19.67 µg/mL and exhibited the most efficacious antioxidant property among the others. The 24-hour methanol extract showed the most promising results on MTT assay. Therefore, CN methanol extract is a promising candidate to proceed in other anticancer studies such as cell cycle arrest and apoptosis assays.
format Article
author Fong, Isabel Lim
Phung, Joyce Hui Yie
Khong, Heng Yen
Ban, Weng Kit
author_facet Fong, Isabel Lim
Phung, Joyce Hui Yie
Khong, Heng Yen
Ban, Weng Kit
author_sort Fong, Isabel Lim
title In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts
title_short In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts
title_full In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts
title_fullStr In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts
title_full_unstemmed In vitro antioxidant and anticancer activities of Clinacanthus nutans extracts
title_sort in vitro antioxidant and anticancer activities of clinacanthus nutans extracts
publisher Science, Engineering and Health Studies (SEHS)
publishDate 2021
url http://ir.unimas.my/id/eprint/41540/1/Isabel%20Fong%20Lim.pdf
http://ir.unimas.my/id/eprint/41540/
https://li01.tci-thaijo.org/index.php/sehs/article/view/248287
https://doi.org/10.14456/sehs.2021.51
_version_ 1761623552348389376
score 13.211869

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