Determination of genetic diversity and genetic relatedness using ISSR markers in Duabanga moluccana
Duabanga moluccana or locally known as Sawih is a widely known forest tree species for its multi-purpose timber and other natural products such as fibers. Genetic diversity is important for the maintenance of the viability and the adaptive potential of populations and species. This information wil...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IJRP.ORG
2018
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/41365/1/Ho%2C%20Wei%20Seng.pdf http://ir.unimas.my/id/eprint/41365/ https://www.ijrp.org/paper-detail/263 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Duabanga moluccana or locally known as Sawih is a widely known forest tree species for its multi-purpose
timber and other natural products such as fibers. Genetic diversity is important for the maintenance of the
viability and the adaptive potential of populations and species. This information will be a basis for
establishing tree improvement programme and for management or conservation of natural communities. The
present study was aimed to assess the genetic diversity and genetic relatedness of D. moluccana trees
collected from three natural forests in Sarawak, namely, Mukah, Tatau and Niah using inter simple sequence
repeat (ISSR) markers. A total of 73 loci from 90 individuals were successfully amplified using three selected
ISSR primers, namely, (AC)10, (ACC)6G and ACG(GT)7. The Shannon’s diversity index showed that D.
moluccana trees in Mukah (0.499) was the most diverse population compared to Tatau (0.380) and Niah
(0.330). Neighbor joining tree was also constructed to determine the genetic relationship among the three D.
moluccana populations. The populations were completely clustered into three main groups, in accordance to
their corresponding population and origin. Based on the results, it implies that D. moluccana trees are
genetically diverse and related both within and among populations. |
|---|