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Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment

Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to the family Pseudomonadaceae. The species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, an...

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Main Authors: Abdullahi, Rakiyah, Samuel, Lihan, Carlos, B.S, Lesley Maurice, Bilung, Kathleen, Michelle Mikal, Felecia, C.
Format: Article
Language:English
Published: Pelagia Research Library 2013
Subjects:
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/40397/3/Detection%20of%20oprL%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/40397/
https://www.primescholars.com/abstract/detection-of-oprl-gene-and-antibiotic-resistance-of-pseudomonas-aeruginosa-from-aquaculture-environment-91865.html
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spelling my.unimas.ir.403972023-05-26T02:12:19Z http://ir.unimas.my/id/eprint/40397/ Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment Abdullahi, Rakiyah Samuel, Lihan Carlos, B.S Lesley Maurice, Bilung Kathleen, Michelle Mikal Felecia, C. QR Microbiology Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to the family Pseudomonadaceae. The species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, and their improper usage poses a risk of potential transfer of resistance from aquaculture bacteria to human and animal pathogens. This study was conducted to isolate P. aeruginosa from fish, prawn and water samples, followed by PCR detection of oprL gene locus. The antibiotic resistance pattern of the isolates was also determined. Based on the results from PCR analysis performed, 13 isolates of P. aeruginosawere isolated. All of the isolates tested were resistance to at least one antibiotic. Highest level of resistance was observed against ampicillin and erythromycin while the lowest was observed against gentamicin, norfloxacin and nalidixic acid. This study suggested that the presence of the bacteria in the aquaculture environment may pose the risk of antibiotic resistance to those who are exposed to the aquaculture environment.Based on the results of this study, it can be said that gentamicin, norflaxin and nalidixic acid can be effective agents for the treatment of P. aeruginosa. Pelagia Research Library 2013 Article PeerReviewed text en http://ir.unimas.my/id/eprint/40397/3/Detection%20of%20oprL%20-%20Copy.pdf Abdullahi, Rakiyah and Samuel, Lihan and Carlos, B.S and Lesley Maurice, Bilung and Kathleen, Michelle Mikal and Felecia, C. (2013) Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment. European Journal of Experimental Biology, 3 (6). pp. 148-152. ISSN 2248 –9215 https://www.primescholars.com/abstract/detection-of-oprl-gene-and-antibiotic-resistance-of-pseudomonas-aeruginosa-from-aquaculture-environment-91865.html
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Abdullahi, Rakiyah
Samuel, Lihan
Carlos, B.S
Lesley Maurice, Bilung
Kathleen, Michelle Mikal
Felecia, C.
Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment
description Pseudomonas aeruginosa is a gram-negative rod shape bacterium belonging to the family Pseudomonadaceae. The species is highly adaptable opportunistic pathogen, capable of surviving in a variety of environment, including aquaculture environment. Antibiotics are used in the aquaculture environment, and their improper usage poses a risk of potential transfer of resistance from aquaculture bacteria to human and animal pathogens. This study was conducted to isolate P. aeruginosa from fish, prawn and water samples, followed by PCR detection of oprL gene locus. The antibiotic resistance pattern of the isolates was also determined. Based on the results from PCR analysis performed, 13 isolates of P. aeruginosawere isolated. All of the isolates tested were resistance to at least one antibiotic. Highest level of resistance was observed against ampicillin and erythromycin while the lowest was observed against gentamicin, norfloxacin and nalidixic acid. This study suggested that the presence of the bacteria in the aquaculture environment may pose the risk of antibiotic resistance to those who are exposed to the aquaculture environment.Based on the results of this study, it can be said that gentamicin, norflaxin and nalidixic acid can be effective agents for the treatment of P. aeruginosa.
format Article
author Abdullahi, Rakiyah
Samuel, Lihan
Carlos, B.S
Lesley Maurice, Bilung
Kathleen, Michelle Mikal
Felecia, C.
author_facet Abdullahi, Rakiyah
Samuel, Lihan
Carlos, B.S
Lesley Maurice, Bilung
Kathleen, Michelle Mikal
Felecia, C.
author_sort Abdullahi, Rakiyah
title Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment
title_short Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment
title_full Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment
title_fullStr Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment
title_full_unstemmed Detection of oprL gene and antibiotic resistance of Pseudomonas aeruginosa from aquaculture environment
title_sort detection of oprl gene and antibiotic resistance of pseudomonas aeruginosa from aquaculture environment
publisher Pelagia Research Library
publishDate 2013
url http://ir.unimas.my/id/eprint/40397/3/Detection%20of%20oprL%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/40397/
https://www.primescholars.com/abstract/detection-of-oprl-gene-and-antibiotic-resistance-of-pseudomonas-aeruginosa-from-aquaculture-environment-91865.html
_version_ 1767209869388021760
score 13.211869

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