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Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA

Metroxylon Sagu also known as sago plants can be found in Malaysia especially in Sarawak. The sago plants serve as starch sources for the locals. The sago plants have large economical value when the plants is trunking. But one day, scientist have found out that the trunking sago palms have diverse i...

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Main Author: Wai, Chee Siong
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
Subjects:
QK Botany
Online Access:http://ir.unimas.my/id/eprint/39926/1/WAI%20CHEE%20SIONG%2024pgs.pdf
http://ir.unimas.my/id/eprint/39926/4/WAI%20CHEE%20SIONG%20ft.pdf
http://ir.unimas.my/id/eprint/39926/
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spelling my.unimas.ir.399262024-09-24T01:17:53Z http://ir.unimas.my/id/eprint/39926/ Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA Wai, Chee Siong QK Botany Metroxylon Sagu also known as sago plants can be found in Malaysia especially in Sarawak. The sago plants serve as starch sources for the locals. The sago plants have large economical value when the plants is trunking. But one day, scientist have found out that the trunking sago palms have diverse into another categories which are non-trunking sago palms. The non-trunking sago palms cause the economical to be reduce from time to time. The study is carry out through expression analysis to identify and differentiate the specific gene via cDNA amplification. Gene of Peroxidase A2 and Catalase was selected to design the primers. The experiment was using CTAB based method to extract the mRNA from the sago leafs. The primer selected purposely designed to apply. Expression level of the peroxidase A2 gene for trunking and non-trunking expressed equally. The expression of catalase gene cannot be expressed due to many reasons. Further research need to be done for confirmation of the related genes. CTAB based method is consume time but can get better results. Universiti Malaysia Sarawak, (UNIMAS) 2022 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/39926/1/WAI%20CHEE%20SIONG%2024pgs.pdf text en http://ir.unimas.my/id/eprint/39926/4/WAI%20CHEE%20SIONG%20ft.pdf Wai, Chee Siong (2022) Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic QK Botany
spellingShingle QK Botany
Wai, Chee Siong
Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA
description Metroxylon Sagu also known as sago plants can be found in Malaysia especially in Sarawak. The sago plants serve as starch sources for the locals. The sago plants have large economical value when the plants is trunking. But one day, scientist have found out that the trunking sago palms have diverse into another categories which are non-trunking sago palms. The non-trunking sago palms cause the economical to be reduce from time to time. The study is carry out through expression analysis to identify and differentiate the specific gene via cDNA amplification. Gene of Peroxidase A2 and Catalase was selected to design the primers. The experiment was using CTAB based method to extract the mRNA from the sago leafs. The primer selected purposely designed to apply. Expression level of the peroxidase A2 gene for trunking and non-trunking expressed equally. The expression of catalase gene cannot be expressed due to many reasons. Further research need to be done for confirmation of the related genes. CTAB based method is consume time but can get better results.
format Final Year Project Report
author Wai, Chee Siong
author_facet Wai, Chee Siong
author_sort Wai, Chee Siong
title Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA
title_short Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA
title_full Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA
title_fullStr Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA
title_full_unstemmed Expression Analysis of Differentiate Sago Palm Gene (Peroxidase A2 and Catalase Gene) via Amplified cDNA
title_sort expression analysis of differentiate sago palm gene (peroxidase a2 and catalase gene) via amplified cdna
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2022
url http://ir.unimas.my/id/eprint/39926/1/WAI%20CHEE%20SIONG%2024pgs.pdf
http://ir.unimas.my/id/eprint/39926/4/WAI%20CHEE%20SIONG%20ft.pdf
http://ir.unimas.my/id/eprint/39926/
_version_ 1811600371812925440
score 13.211869

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