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Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs)

Foods contain fats, oils, and greases (FOGs) are commonly washed down into the kitchen sink and sticks to the inner walls of kitchen pipes, drains and sewer pipes and forming clogs which causes the blockage that restrict the flow of wastewater. Waste FOGs material sometimes flows directly to the mai...

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Bibliographic Details
Main Author: Soonali, Wasdewa
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
Subjects:
QA Mathematics
QH Natural history
QR Microbiology
Online Access:http://ir.unimas.my/id/eprint/39908/1/SOONALI%20AP%20WASDEWA%2024pgs.pdf
http://ir.unimas.my/id/eprint/39908/4/Soonali%20Wasdewa%20ft.pdf
http://ir.unimas.my/id/eprint/39908/
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spelling my.unimas.ir.399082024-07-31T06:23:30Z http://ir.unimas.my/id/eprint/39908/ Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs) Soonali, Wasdewa QA Mathematics QH Natural history QR Microbiology Foods contain fats, oils, and greases (FOGs) are commonly washed down into the kitchen sink and sticks to the inner walls of kitchen pipes, drains and sewer pipes and forming clogs which causes the blockage that restrict the flow of wastewater. Waste FOGs material sometimes flows directly to the mainstream and forms an oil layer which give threat to aquatic organisms by reducing the dissolved oxygen level. The biosurfactant producing bacteria act as biodegradation agent to emulsify the FOG materials from clogging and prevent blockage in the drains and sewage system. This study therefore aims to isolate and identify the biosurfactant producing bacteria. The oil spreading assay and emulsification index (E24) test were used to determine the presence of biosurfactant and Bacillus paramycoides and Bacillus cereus were identified via comparison of the isolates’16S rRNA with deposited sequences in NCBI GenBank. Bacillus paramycoides and Bacillus cereus produced largest diameter of clearing zone compared to other biosurfactant producing bacteria on the oil spreading assay which indicated as the high quality of biosurfactants produced. Biosurfactant screening via emulsification index (E24) increased with presence of biosurfactants produced by B. paramycoides and Bacillus cereus due to the high interaction between water and oil which have industrial potential with greater ability in biodegrading the FOGs materials. Universiti Malaysia Sarawak, (UNIMAS) 2022 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/39908/1/SOONALI%20AP%20WASDEWA%2024pgs.pdf text en http://ir.unimas.my/id/eprint/39908/4/Soonali%20Wasdewa%20ft.pdf Soonali, Wasdewa (2022) Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs). [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic QA Mathematics
QH Natural history
QR Microbiology
spellingShingle QA Mathematics
QH Natural history
QR Microbiology
Soonali, Wasdewa
Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs)
description Foods contain fats, oils, and greases (FOGs) are commonly washed down into the kitchen sink and sticks to the inner walls of kitchen pipes, drains and sewer pipes and forming clogs which causes the blockage that restrict the flow of wastewater. Waste FOGs material sometimes flows directly to the mainstream and forms an oil layer which give threat to aquatic organisms by reducing the dissolved oxygen level. The biosurfactant producing bacteria act as biodegradation agent to emulsify the FOG materials from clogging and prevent blockage in the drains and sewage system. This study therefore aims to isolate and identify the biosurfactant producing bacteria. The oil spreading assay and emulsification index (E24) test were used to determine the presence of biosurfactant and Bacillus paramycoides and Bacillus cereus were identified via comparison of the isolates’16S rRNA with deposited sequences in NCBI GenBank. Bacillus paramycoides and Bacillus cereus produced largest diameter of clearing zone compared to other biosurfactant producing bacteria on the oil spreading assay which indicated as the high quality of biosurfactants produced. Biosurfactant screening via emulsification index (E24) increased with presence of biosurfactants produced by B. paramycoides and Bacillus cereus due to the high interaction between water and oil which have industrial potential with greater ability in biodegrading the FOGs materials.
format Final Year Project Report
author Soonali, Wasdewa
author_facet Soonali, Wasdewa
author_sort Soonali, Wasdewa
title Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs)
title_short Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs)
title_full Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs)
title_fullStr Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs)
title_full_unstemmed Isolation and Identification of Biosurfactant Producing Bacteria from Fats, Oils and Greases (FOGs)
title_sort isolation and identification of biosurfactant producing bacteria from fats, oils and greases (fogs)
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2022
url http://ir.unimas.my/id/eprint/39908/1/SOONALI%20AP%20WASDEWA%2024pgs.pdf
http://ir.unimas.my/id/eprint/39908/4/Soonali%20Wasdewa%20ft.pdf
http://ir.unimas.my/id/eprint/39908/
_version_ 1806456035695656960
score 13.211869

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