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Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring

Bacteria living in the soil are capable of synthesizing different type of enzymes that are vital in many biochemical processes. Amylase, known as starch hydrolysing enzyme, is one of indispensable enzyme which can be naturally produced by many bacterial species. The discoveries of the amylolytic mi...

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Main Author: Sharifah Nurain, Hussain
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
Subjects:
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/39899/1/SHARIFAH%20NURAIN%20BINTI%20HUSSAIN%2024pgs.pdf
http://ir.unimas.my/id/eprint/39899/4/Sharifah%20Nurain%20ft.pdf
http://ir.unimas.my/id/eprint/39899/
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spelling my.unimas.ir.398992024-07-31T03:07:00Z http://ir.unimas.my/id/eprint/39899/ Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring Sharifah Nurain, Hussain S Agriculture (General) Bacteria living in the soil are capable of synthesizing different type of enzymes that are vital in many biochemical processes. Amylase, known as starch hydrolysing enzyme, is one of indispensable enzyme which can be naturally produced by many bacterial species. The discoveries of the amylolytic microbial species will help to boost the advancement of enzyme exploitation in many biotechnological based industries especially in Sarawak. Thus, the aim of this research is to isolate the pure microbial strain from the soil sample at selected site of Panchor Hot Spring and screen for the potential amylase activity. In this study, three pure isolates were successfully isolated using nutrient agar and all tested isolates showed amylase activity when screened with starch hydrolysis assay. The rate of amylase activity of amylolytic strains was analysed by using 3,5- dinitrosalicylic (DNS) assay resulting in the highest activity by E1 isolate which was 143.85 U/ml. In conclusion, amylase producing bacteria was successfully isolated from soil sample of Panchor Hot Spring, however its enzymatic potential must be further studied before it can further be utilised for many industrial applications. Universiti Malaysia Sarawak, (UNIMAS) 2022 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/39899/1/SHARIFAH%20NURAIN%20BINTI%20HUSSAIN%2024pgs.pdf text en http://ir.unimas.my/id/eprint/39899/4/Sharifah%20Nurain%20ft.pdf Sharifah Nurain, Hussain (2022) Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Sharifah Nurain, Hussain
Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring
description Bacteria living in the soil are capable of synthesizing different type of enzymes that are vital in many biochemical processes. Amylase, known as starch hydrolysing enzyme, is one of indispensable enzyme which can be naturally produced by many bacterial species. The discoveries of the amylolytic microbial species will help to boost the advancement of enzyme exploitation in many biotechnological based industries especially in Sarawak. Thus, the aim of this research is to isolate the pure microbial strain from the soil sample at selected site of Panchor Hot Spring and screen for the potential amylase activity. In this study, three pure isolates were successfully isolated using nutrient agar and all tested isolates showed amylase activity when screened with starch hydrolysis assay. The rate of amylase activity of amylolytic strains was analysed by using 3,5- dinitrosalicylic (DNS) assay resulting in the highest activity by E1 isolate which was 143.85 U/ml. In conclusion, amylase producing bacteria was successfully isolated from soil sample of Panchor Hot Spring, however its enzymatic potential must be further studied before it can further be utilised for many industrial applications.
format Final Year Project Report
author Sharifah Nurain, Hussain
author_facet Sharifah Nurain, Hussain
author_sort Sharifah Nurain, Hussain
title Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring
title_short Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring
title_full Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring
title_fullStr Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring
title_full_unstemmed Screening of Amylase Activity of Bacteria Isolated from Soil Sample of Panchor Hot Spring
title_sort screening of amylase activity of bacteria isolated from soil sample of panchor hot spring
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2022
url http://ir.unimas.my/id/eprint/39899/1/SHARIFAH%20NURAIN%20BINTI%20HUSSAIN%2024pgs.pdf
http://ir.unimas.my/id/eprint/39899/4/Sharifah%20Nurain%20ft.pdf
http://ir.unimas.my/id/eprint/39899/
_version_ 1806456035058122752
score 13.211869

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