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Screening of medicinal plants against A. flavus

Infestation of Aspergillus flavus has been a threat to the important crops and human's health. The usage of synthetic fungicides have resulted into environmental and health issues hence making natural fungicides a more attractive option. This study aims to investigate the effectiveness of Cym...

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Main Author: Nur Amalin Amni, Mahmud
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
Subjects:
TP Chemical technology
Online Access:http://ir.unimas.my/id/eprint/39874/1/NUR%20AMALIN%20AMNI%20BINTI%20MAHMUD%2024pgs.pdf
http://ir.unimas.my/id/eprint/39874/2/NUR%20AMALIN%20AMNI%20BINTI%20MAHMUD%20ft.pdf
http://ir.unimas.my/id/eprint/39874/
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spelling my.unimas.ir.398742022-09-20T09:33:12Z http://ir.unimas.my/id/eprint/39874/ Screening of medicinal plants against A. flavus Nur Amalin Amni, Mahmud TP Chemical technology Infestation of Aspergillus flavus has been a threat to the important crops and human's health. The usage of synthetic fungicides have resulted into environmental and health issues hence making natural fungicides a more attractive option. This study aims to investigate the effectiveness of Cympbogon citratus and Nigella sativa against toxigenic Aspergillus flavus growth. C.citratus and N. sativa were purchased at Kota Samarahan. The leaves and seeds were dried at 60 °C for one to two days in drying oven. The dried leaves and seeds were then ground by using laboratory blender and extracted with 96% of ethanol. A. flavus was sub-cultured on potato dextrose agar (PDA). In this experiment, disc diffusion technique was used as antifungal assay where the extracts were diluted in DMSO and water resulting in 100%, 75%, 50% and 25% of the ethanolic extract of the plants.. Then, the plates were incubated at 72 hours at 37 °C in inverted position. The diameter of inhibition zone was measured in mm as antifungal activity. Results obtained suggest that both plants show the antifungal activity with 100%, 75, and 50% concentration of extracts. The higher the amount of extracts used, the higher the antifungal activity exhibited towards the fungi. However, at 25% concentration of extracts, neither plants show any antifungal activity. Last but not least, C. citratus exhibited higher antifungal activity compared to N. sativa. Universiti Malaysia Sarawak, (UNIMAS) 2022 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/39874/1/NUR%20AMALIN%20AMNI%20BINTI%20MAHMUD%2024pgs.pdf text en http://ir.unimas.my/id/eprint/39874/2/NUR%20AMALIN%20AMNI%20BINTI%20MAHMUD%20ft.pdf Nur Amalin Amni, Mahmud (2022) Screening of medicinal plants against A. flavus. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nur Amalin Amni, Mahmud
Screening of medicinal plants against A. flavus
description Infestation of Aspergillus flavus has been a threat to the important crops and human's health. The usage of synthetic fungicides have resulted into environmental and health issues hence making natural fungicides a more attractive option. This study aims to investigate the effectiveness of Cympbogon citratus and Nigella sativa against toxigenic Aspergillus flavus growth. C.citratus and N. sativa were purchased at Kota Samarahan. The leaves and seeds were dried at 60 °C for one to two days in drying oven. The dried leaves and seeds were then ground by using laboratory blender and extracted with 96% of ethanol. A. flavus was sub-cultured on potato dextrose agar (PDA). In this experiment, disc diffusion technique was used as antifungal assay where the extracts were diluted in DMSO and water resulting in 100%, 75%, 50% and 25% of the ethanolic extract of the plants.. Then, the plates were incubated at 72 hours at 37 °C in inverted position. The diameter of inhibition zone was measured in mm as antifungal activity. Results obtained suggest that both plants show the antifungal activity with 100%, 75, and 50% concentration of extracts. The higher the amount of extracts used, the higher the antifungal activity exhibited towards the fungi. However, at 25% concentration of extracts, neither plants show any antifungal activity. Last but not least, C. citratus exhibited higher antifungal activity compared to N. sativa.
format Final Year Project Report
author Nur Amalin Amni, Mahmud
author_facet Nur Amalin Amni, Mahmud
author_sort Nur Amalin Amni, Mahmud
title Screening of medicinal plants against A. flavus
title_short Screening of medicinal plants against A. flavus
title_full Screening of medicinal plants against A. flavus
title_fullStr Screening of medicinal plants against A. flavus
title_full_unstemmed Screening of medicinal plants against A. flavus
title_sort screening of medicinal plants against a. flavus
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2022
url http://ir.unimas.my/id/eprint/39874/1/NUR%20AMALIN%20AMNI%20BINTI%20MAHMUD%2024pgs.pdf
http://ir.unimas.my/id/eprint/39874/2/NUR%20AMALIN%20AMNI%20BINTI%20MAHMUD%20ft.pdf
http://ir.unimas.my/id/eprint/39874/
_version_ 1744652144735158272
score 13.211869

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