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Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA

Metroxylon Sagu (Sago palm) is an important tropical plant and important starch source in Malaysia. However, the presence of the stunted phenotype of sago palm had largely reduced the economic value of the plant and the problem remains one of the major concerns of the whole sago plantation till thes...

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Main Author: LIEYEU, CHIOK
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
Subjects:
QK Botany
Online Access:http://ir.unimas.my/id/eprint/39833/1/LIEYEU%20CHIOK%20ft.pdf
http://ir.unimas.my/id/eprint/39833/
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spelling my.unimas.ir.398332023-11-22T04:17:06Z http://ir.unimas.my/id/eprint/39833/ Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA LIEYEU, CHIOK QK Botany Metroxylon Sagu (Sago palm) is an important tropical plant and important starch source in Malaysia. However, the presence of the stunted phenotype of sago palm had largely reduced the economic value of the plant and the problem remains one of the major concerns of the whole sago plantation till these days. The present study looks at expression analysis of differentiated sago palm gene using amplified cDNA. The Phosphate transporter gene and Detoxification protein gene were selected to be used to design the primer for amplifying the cDNA. CTAB-buffer was used to extract the total mRNA from the sago leaf. The expression level of the phosphate transporter was found to be similar between the trunking and non-trunking species of the sago palm. While the expression of the detoxification protein was showed poorly expressed in trunking species and not expressed in the non-trunking species. Further research on the above-mentioned factors for Metroxylon sagu should be conducted due to its high potential in starch production and commercialized products. Universiti Malaysia Sarawak, (UNIMAS) 2022 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/39833/1/LIEYEU%20CHIOK%20ft.pdf LIEYEU, CHIOK (2022) Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QK Botany
spellingShingle QK Botany
LIEYEU, CHIOK
Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA
description Metroxylon Sagu (Sago palm) is an important tropical plant and important starch source in Malaysia. However, the presence of the stunted phenotype of sago palm had largely reduced the economic value of the plant and the problem remains one of the major concerns of the whole sago plantation till these days. The present study looks at expression analysis of differentiated sago palm gene using amplified cDNA. The Phosphate transporter gene and Detoxification protein gene were selected to be used to design the primer for amplifying the cDNA. CTAB-buffer was used to extract the total mRNA from the sago leaf. The expression level of the phosphate transporter was found to be similar between the trunking and non-trunking species of the sago palm. While the expression of the detoxification protein was showed poorly expressed in trunking species and not expressed in the non-trunking species. Further research on the above-mentioned factors for Metroxylon sagu should be conducted due to its high potential in starch production and commercialized products.
format Final Year Project Report
author LIEYEU, CHIOK
author_facet LIEYEU, CHIOK
author_sort LIEYEU, CHIOK
title Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA
title_short Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA
title_full Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA
title_fullStr Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA
title_full_unstemmed Expression Analysis of Differentiated Sago Palm gene (Phosphate transporter and Detoxification Protein) via Amplified cDNA
title_sort expression analysis of differentiated sago palm gene (phosphate transporter and detoxification protein) via amplified cdna
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2022
url http://ir.unimas.my/id/eprint/39833/1/LIEYEU%20CHIOK%20ft.pdf
http://ir.unimas.my/id/eprint/39833/
_version_ 1783883543360831488
score 13.211869

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