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Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines

Colorectal carcinoma, or colorectal cancer (CRC), is a type of cancer that affects the colon or rectal area and it is likely to be related with TSG101 gene expression. TSG101 is a gene that encodes a multi-domain protein that is homologous to an inactive ubiquitin-conjugating enzyme and it may act a...

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Main Author: Bonnie, Tang Bao Chee
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
Subjects:
RC Internal medicine
Online Access:http://ir.unimas.my/id/eprint/39790/1/BONNIE%20TANG%20BAO%20CHEE%2024pgs.pdf
http://ir.unimas.my/id/eprint/39790/4/BONNIE%20TANG%20%20ft.pdf
http://ir.unimas.my/id/eprint/39790/
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spelling my.unimas.ir.397902023-02-10T03:01:04Z http://ir.unimas.my/id/eprint/39790/ Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines Bonnie, Tang Bao Chee RC Internal medicine Colorectal carcinoma, or colorectal cancer (CRC), is a type of cancer that affects the colon or rectal area and it is likely to be related with TSG101 gene expression. TSG101 is a gene that encodes a multi-domain protein that is homologous to an inactive ubiquitin-conjugating enzyme and it may act as a positive enhancer in carcinogenesis and CRC progression. In the recent finding on the TSG101 has shown to be high expression in CRC, but there are no studies that have been done in determining and analyzing the gene sequences of TSG101 in CRC cell lines. The objectives of this study are to determine the existence of TSG101 in CRC cell lines and to perform In Silico Characterization of TSG101 in CRC cell lines. The starting materials of this study were the total RNA that was isolated from HCT116 and HT29 cell lines, and they were reverse transcribed into cDNA to perform PCR. Lastly, the amplified products proceeded for sequencing and analysis. However, due to some unavoidable errors, we could not send our PCR product on time. The PCR products showed expression in the expected size range (200-300bp), hence the products were likely to be TSG101 in HCT116 and HT29 cell lines.Therefore, we subjected the expected amplified sequence (NM_006292.4) on CD search and we found out that the predicted amplified product is located at the nucleotide 499 to 739 in TSG101 and it is a region of UEV domain. Universiti Malaysia Sarawak, (UNIMAS) 2022 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/39790/1/BONNIE%20TANG%20BAO%20CHEE%2024pgs.pdf text en http://ir.unimas.my/id/eprint/39790/4/BONNIE%20TANG%20%20ft.pdf Bonnie, Tang Bao Chee (2022) Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic RC Internal medicine
spellingShingle RC Internal medicine
Bonnie, Tang Bao Chee
Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines
description Colorectal carcinoma, or colorectal cancer (CRC), is a type of cancer that affects the colon or rectal area and it is likely to be related with TSG101 gene expression. TSG101 is a gene that encodes a multi-domain protein that is homologous to an inactive ubiquitin-conjugating enzyme and it may act as a positive enhancer in carcinogenesis and CRC progression. In the recent finding on the TSG101 has shown to be high expression in CRC, but there are no studies that have been done in determining and analyzing the gene sequences of TSG101 in CRC cell lines. The objectives of this study are to determine the existence of TSG101 in CRC cell lines and to perform In Silico Characterization of TSG101 in CRC cell lines. The starting materials of this study were the total RNA that was isolated from HCT116 and HT29 cell lines, and they were reverse transcribed into cDNA to perform PCR. Lastly, the amplified products proceeded for sequencing and analysis. However, due to some unavoidable errors, we could not send our PCR product on time. The PCR products showed expression in the expected size range (200-300bp), hence the products were likely to be TSG101 in HCT116 and HT29 cell lines.Therefore, we subjected the expected amplified sequence (NM_006292.4) on CD search and we found out that the predicted amplified product is located at the nucleotide 499 to 739 in TSG101 and it is a region of UEV domain.
format Final Year Project Report
author Bonnie, Tang Bao Chee
author_facet Bonnie, Tang Bao Chee
author_sort Bonnie, Tang Bao Chee
title Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines
title_short Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines
title_full Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines
title_fullStr Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines
title_full_unstemmed Gene Sequence Analysis of Tumour Susceptibility Gene (TSG101) in Colereactal Carcinoma Cell Lines
title_sort gene sequence analysis of tumour susceptibility gene (tsg101) in colereactal carcinoma cell lines
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2022
url http://ir.unimas.my/id/eprint/39790/1/BONNIE%20TANG%20BAO%20CHEE%2024pgs.pdf
http://ir.unimas.my/id/eprint/39790/4/BONNIE%20TANG%20%20ft.pdf
http://ir.unimas.my/id/eprint/39790/
_version_ 1758582583394828288
score 13.211869

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