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Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages

Tor tambroides is an economically important freshwater fish from the Cyprinidae family of mahseers. Despite its substantial economic value, T. tambroides has received very little fundamental scientific attention with the exception of several existing studies on their growth performance and feeding i...

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Main Author: Fatin Emilya Wahida, Pauji
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2022
Subjects:
QA Mathematics
Online Access:http://ir.unimas.my/id/eprint/39576/1/Fatin%20Emilya%20Wahida%20Pauji%20ft.pdf
http://ir.unimas.my/id/eprint/39576/
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spelling my.unimas.ir.395762023-08-15T03:58:59Z http://ir.unimas.my/id/eprint/39576/ Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages Fatin Emilya Wahida, Pauji QA Mathematics Tor tambroides is an economically important freshwater fish from the Cyprinidae family of mahseers. Despite its substantial economic value, T. tambroides has received very little fundamental scientific attention with the exception of several existing studies on their growth performance and feeding in confined environments. The aim of this study is to assess and compare the gene expression of growth-related genes in T. tambroides with a focus on gaining insights into the modulation of the somatotropic axis and its components in distinct early developmental stages. The overall gene expression of gh, igf1 and igf1R were evaluated by semi-quantitative PCR with β-actin serving a housekeeping gene. To achieve the desired gene expression, the primer pairs of gh, igf1 and igf1R targeting main functional domain of somatotropin, superfamily of insulin and fibronectin type-III (Fn3), respectively was tested in hatchlings and three different consequential organ such as brain, liver and muscle in juveniles T. tambroides. The size of the gene amplified for gh is 600bp for every fish sample which is close to its expected size (587bp) and igf1 yield at about 450bp. However, the expression level of igf1R is much higher than expected at only 700bp as compared to the expected size at 525bp. As one would expect, the differences in the growth-governing endocrinology system during larval development and early growth affect the expression of growth-related genes with the GH/IGF1 system are proven to play pivotal roles in fish growth. Universiti Malaysia Sarawak, (UNIMAS) 2022 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/39576/1/Fatin%20Emilya%20Wahida%20Pauji%20ft.pdf Fatin Emilya Wahida, Pauji (2022) Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QA Mathematics
spellingShingle QA Mathematics
Fatin Emilya Wahida, Pauji
Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages
description Tor tambroides is an economically important freshwater fish from the Cyprinidae family of mahseers. Despite its substantial economic value, T. tambroides has received very little fundamental scientific attention with the exception of several existing studies on their growth performance and feeding in confined environments. The aim of this study is to assess and compare the gene expression of growth-related genes in T. tambroides with a focus on gaining insights into the modulation of the somatotropic axis and its components in distinct early developmental stages. The overall gene expression of gh, igf1 and igf1R were evaluated by semi-quantitative PCR with β-actin serving a housekeeping gene. To achieve the desired gene expression, the primer pairs of gh, igf1 and igf1R targeting main functional domain of somatotropin, superfamily of insulin and fibronectin type-III (Fn3), respectively was tested in hatchlings and three different consequential organ such as brain, liver and muscle in juveniles T. tambroides. The size of the gene amplified for gh is 600bp for every fish sample which is close to its expected size (587bp) and igf1 yield at about 450bp. However, the expression level of igf1R is much higher than expected at only 700bp as compared to the expected size at 525bp. As one would expect, the differences in the growth-governing endocrinology system during larval development and early growth affect the expression of growth-related genes with the GH/IGF1 system are proven to play pivotal roles in fish growth.
format Final Year Project Report
author Fatin Emilya Wahida, Pauji
author_facet Fatin Emilya Wahida, Pauji
author_sort Fatin Emilya Wahida, Pauji
title Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages
title_short Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages
title_full Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages
title_fullStr Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages
title_full_unstemmed Expression Analysis of Growth-Related Genes in Empurau (Tor Tambroides) During Early Developmental Stages
title_sort expression analysis of growth-related genes in empurau (tor tambroides) during early developmental stages
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2022
url http://ir.unimas.my/id/eprint/39576/1/Fatin%20Emilya%20Wahida%20Pauji%20ft.pdf
http://ir.unimas.my/id/eprint/39576/
_version_ 1775627316331806720
score 13.211869

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