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Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture

Morphological and genetic characterization of MCBC1, PBC230, KKM22 and KKM4 cocoa clones derived from staminode and immature zygotic embryo culture were compared with those conventionally grafted. Somatic embryogenesis culture successfully produced true-to-type progenies of elite cocoa clones of MCB...

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Main Authors: Gibson, Entuni, Hollena, Nori, Rebicca, Edward, Ahmad Kamil, Bin Mohd Jaafar
Format: Article
Language:English
Published: Walailak University 2022
Subjects:
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/39270/2/Agronomic%20Traits%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/39270/
https://tis.wu.ac.th/index.php/tis
https://doi.org/10.48048/tis.2022.5614
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spelling my.unimas.ir.392702022-08-19T07:35:38Z http://ir.unimas.my/id/eprint/39270/ Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture Gibson, Entuni Hollena, Nori Rebicca, Edward Ahmad Kamil, Bin Mohd Jaafar S Agriculture (General) Morphological and genetic characterization of MCBC1, PBC230, KKM22 and KKM4 cocoa clones derived from staminode and immature zygotic embryo culture were compared with those conventionally grafted. Somatic embryogenesis culture successfully produced true-to-type progenies of elite cocoa clones of MCBC1, PBC230, KKM22 and KKM4 (from staminode culture). Phenotype variations (p < 0.05) were observed only in KKM4 clone from immature zygotic embryo culture which exhibited lower quantities in the fresh pod weight, number of flat beans per pod, seed length, seed width and individual seed weight. The genetic stability of the cultured clones was tested using fragment analysis with 12 SSR primers to validate these results. Eleven of these SSR primers detected mutations only in the allelic profiles of KKM4 clone from immature zygotic embryo. These results validated those variations in KKM4 clones of immature zygotic embryo culture were due to interactions between genotypic and explant types. Unfortunately, these variations were negative attributes to cocoa productivity. Thus, it is suggested that successful production of true-to-type KKM4 cocoa clone should consider other means of propagation including modification of the culture conditions. Walailak University 2022-08-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/39270/2/Agronomic%20Traits%20-%20Copy.pdf Gibson, Entuni and Hollena, Nori and Rebicca, Edward and Ahmad Kamil, Bin Mohd Jaafar (2022) Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture. Trends in Sciences, 19 (15). pp. 1-12. ISSN 2774-0226 (Online) https://tis.wu.ac.th/index.php/tis https://doi.org/10.48048/tis.2022.5614
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Gibson, Entuni
Hollena, Nori
Rebicca, Edward
Ahmad Kamil, Bin Mohd Jaafar
Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture
description Morphological and genetic characterization of MCBC1, PBC230, KKM22 and KKM4 cocoa clones derived from staminode and immature zygotic embryo culture were compared with those conventionally grafted. Somatic embryogenesis culture successfully produced true-to-type progenies of elite cocoa clones of MCBC1, PBC230, KKM22 and KKM4 (from staminode culture). Phenotype variations (p < 0.05) were observed only in KKM4 clone from immature zygotic embryo culture which exhibited lower quantities in the fresh pod weight, number of flat beans per pod, seed length, seed width and individual seed weight. The genetic stability of the cultured clones was tested using fragment analysis with 12 SSR primers to validate these results. Eleven of these SSR primers detected mutations only in the allelic profiles of KKM4 clone from immature zygotic embryo. These results validated those variations in KKM4 clones of immature zygotic embryo culture were due to interactions between genotypic and explant types. Unfortunately, these variations were negative attributes to cocoa productivity. Thus, it is suggested that successful production of true-to-type KKM4 cocoa clone should consider other means of propagation including modification of the culture conditions.
format Article
author Gibson, Entuni
Hollena, Nori
Rebicca, Edward
Ahmad Kamil, Bin Mohd Jaafar
author_facet Gibson, Entuni
Hollena, Nori
Rebicca, Edward
Ahmad Kamil, Bin Mohd Jaafar
author_sort Gibson, Entuni
title Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture
title_short Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture
title_full Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture
title_fullStr Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture
title_full_unstemmed Agronomic Traits and Genetic Fidelity of Four Cocoa Clones Derived from Somatic Embryogenesis Culture
title_sort agronomic traits and genetic fidelity of four cocoa clones derived from somatic embryogenesis culture
publisher Walailak University
publishDate 2022
url http://ir.unimas.my/id/eprint/39270/2/Agronomic%20Traits%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/39270/
https://tis.wu.ac.th/index.php/tis
https://doi.org/10.48048/tis.2022.5614
_version_ 1743110946153299968
score 13.211869

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