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Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7

A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint amon...

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Main Authors: Ooi, Wai Ling, Son, Radu, Gulam, Rusul, Mohamed Ismail, Abdul Karim, Endang, Purwati, Samuel, Lihan
Format: Article
Language:English
Published: Capricon Publication 2000
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/39249/1/2000_Enterobacterial.PDF
http://ir.unimas.my/id/eprint/39249/
https://scialert.net/abstract/?doi=pjbs.2000.35.37
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spelling my.unimas.ir.392492022-08-18T03:20:46Z http://ir.unimas.my/id/eprint/39249/ Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7 Ooi, Wai Ling Son, Radu Gulam, Rusul Mohamed Ismail, Abdul Karim Endang, Purwati Samuel, Lihan Q Science (General) A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination. Capricon Publication 2000 Article PeerReviewed text en http://ir.unimas.my/id/eprint/39249/1/2000_Enterobacterial.PDF Ooi, Wai Ling and Son, Radu and Gulam, Rusul and Mohamed Ismail, Abdul Karim and Endang, Purwati and Samuel, Lihan (2000) Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7. Pakistan Journal of Biological Sciences, 3 (1). pp. 35-37. ISSN 1812-5735 https://scialert.net/abstract/?doi=pjbs.2000.35.37 DOI: 10.3923/pjbs.2000.35.37
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Ooi, Wai Ling
Son, Radu
Gulam, Rusul
Mohamed Ismail, Abdul Karim
Endang, Purwati
Samuel, Lihan
Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7
description A total of 30 strains of Escherichia coli O157:H7 isolated from beef and chicken burger were characterized by Enterobacterial Repetitive Intragenic Consensus (ERIC) genotyping. The ERIC polymorphism patterns obtained as illustrated in a dendrogram showed a significant discriminatory fingerprint among the 30 E. coli O157:H7 strains. Nearly every isolates had a unique fingerprint and that there were no bands that were highly conserved among the isolates. This study suggests that there is considerable genetic heterogeneity among the E. coli O157:H7 strains by ERIC PCR, and that this has application in screening strains from clinical or food samples to detect a virulent strain with a known fingerprint, and to trace its dissemination.
format Article
author Ooi, Wai Ling
Son, Radu
Gulam, Rusul
Mohamed Ismail, Abdul Karim
Endang, Purwati
Samuel, Lihan
author_facet Ooi, Wai Ling
Son, Radu
Gulam, Rusul
Mohamed Ismail, Abdul Karim
Endang, Purwati
Samuel, Lihan
author_sort Ooi, Wai Ling
title Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7
title_short Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7
title_full Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7
title_fullStr Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7
title_full_unstemmed Enterobacterial Repetitive Intragenic Consensus (ERIC) Genotyping of Escherichia coli O157:H7
title_sort enterobacterial repetitive intragenic consensus (eric) genotyping of escherichia coli o157:h7
publisher Capricon Publication
publishDate 2000
url http://ir.unimas.my/id/eprint/39249/1/2000_Enterobacterial.PDF
http://ir.unimas.my/id/eprint/39249/
https://scialert.net/abstract/?doi=pjbs.2000.35.37
_version_ 1743110944077119488
score 13.211869

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