Staff View: Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia

Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia

Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sedimen...

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Main Authors:	Shing Wei, Siew, Mei Yen, Choo, Ian P.G, Marshall, Hazrulrizawati, Abd Hamid, Shamrulazhar, Shamzir Kamal, Sandris Nielsen, Dennis, Hajar Fauzan, Ahmad
Format:	Article
Language:	English
Published:	Elsevier Science, Ltd. 2022
Subjects:	Q Science (General)
Online Access:	http://ir.unimas.my/id/eprint/39239/1/Gut%20microbiome%20-%20Copy1.pdf http://ir.unimas.my/id/eprint/39239/ https://www.sciencedirect.com/science/article/abs/pii/S0025326X22007044?casa_token=0OOmR5CJ-iIAAAAA:NjG9v4Ad1KHNecVl7XVk51xCi_243UKotAo1Oj148m0ra2qk_KQ9hZ-axnkn8i6y6xfTtT0_uA#! https://doi.org/10.1016/j.marpolbul.2022.114022
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id	my.unimas.ir.39239
record_format	eprints
spelling	my.unimas.ir.392392022-08-17T07:03:44Z http://ir.unimas.my/id/eprint/39239/ Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia Shing Wei, Siew Mei Yen, Choo Ian P.G, Marshall Hazrulrizawati, Abd Hamid Shamrulazhar, Shamzir Kamal Sandris Nielsen, Dennis Hajar Fauzan, Ahmad Q Science (General) Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sediment pollution monitoring. The composition of the bacteria communities was determined using 16S rRNA V3-region gene amplicon sequencing, while hybrid whole-genome sequencing was employed to analyze the genome of Vibrio parahaemolyticus. The trace elements and antibiotic compositions were access using high-throughput spectrometry. The alpha- and beta-diversity of bacteria in gut and sediment samples from Kuantan differed substantially within (p-value = 0.017604) and between samples (p-value <0.007), respectively. Vibrio genera predominated in Kuantan samples, while Flavobacterium and Synechococcus_E genera predominated in Pekan samples. Vibrio parahaemolyticus revealed the presence of tet(35) and blaCARB-33 genes that conceived resistance towards tetracycline and betalactam antibiotics, respectively, which were detected in sediment and gut samples. Elsevier Science, Ltd. 2022 Article PeerReviewed text en http://ir.unimas.my/id/eprint/39239/1/Gut%20microbiome%20-%20Copy1.pdf Shing Wei, Siew and Mei Yen, Choo and Ian P.G, Marshall and Hazrulrizawati, Abd Hamid and Shamrulazhar, Shamzir Kamal and Sandris Nielsen, Dennis and Hajar Fauzan, Ahmad (2022) Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia. Marine Pollution Bulletin, 182 (114022). pp. 1-15. ISSN 0025-326X https://www.sciencedirect.com/science/article/abs/pii/S0025326X22007044?casa_token=0OOmR5CJ-iIAAAAA:NjG9v4Ad1KHNecVl7XVk51xCi_243UKotAo1Oj148m0ra2qk_KQ9hZ-axnkn8i6y6xfTtT0_uA#! https://doi.org/10.1016/j.marpolbul.2022.114022
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Shing Wei, Siew Mei Yen, Choo Ian P.G, Marshall Hazrulrizawati, Abd Hamid Shamrulazhar, Shamzir Kamal Sandris Nielsen, Dennis Hajar Fauzan, Ahmad Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
description	Antibiotic contamination in the marine environment forms an emerging threat to marine ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus ocellatus with sediments between two coastal districts of Pahang, which potentially conferring as putative biomarkers for sediment pollution monitoring. The composition of the bacteria communities was determined using 16S rRNA V3-region gene amplicon sequencing, while hybrid whole-genome sequencing was employed to analyze the genome of Vibrio parahaemolyticus. The trace elements and antibiotic compositions were access using high-throughput spectrometry. The alpha- and beta-diversity of bacteria in gut and sediment samples from Kuantan differed substantially within (p-value = 0.017604) and between samples (p-value <0.007), respectively. Vibrio genera predominated in Kuantan samples, while Flavobacterium and Synechococcus_E genera predominated in Pekan samples. Vibrio parahaemolyticus revealed the presence of tet(35) and blaCARB-33 genes that conceived resistance towards tetracycline and betalactam antibiotics, respectively, which were detected in sediment and gut samples.
format	Article
author	Shing Wei, Siew Mei Yen, Choo Ian P.G, Marshall Hazrulrizawati, Abd Hamid Shamrulazhar, Shamzir Kamal Sandris Nielsen, Dennis Hajar Fauzan, Ahmad
author_facet	Shing Wei, Siew Mei Yen, Choo Ian P.G, Marshall Hazrulrizawati, Abd Hamid Shamrulazhar, Shamzir Kamal Sandris Nielsen, Dennis Hajar Fauzan, Ahmad
author_sort	Shing Wei, Siew
title	Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
title_short	Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
title_full	Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
title_fullStr	Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
title_full_unstemmed	Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia
title_sort	gut microbiome and metabolome of sea cucumber (stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in pahang, malaysia
publisher	Elsevier Science, Ltd.
publishDate	2022
url	http://ir.unimas.my/id/eprint/39239/1/Gut%20microbiome%20-%20Copy1.pdf http://ir.unimas.my/id/eprint/39239/ https://www.sciencedirect.com/science/article/abs/pii/S0025326X22007044?casa_token=0OOmR5CJ-iIAAAAA:NjG9v4Ad1KHNecVl7XVk51xCi_243UKotAo1Oj148m0ra2qk_KQ9hZ-axnkn8i6y6xfTtT0_uA#! https://doi.org/10.1016/j.marpolbul.2022.114022
_version_	1743110942983454720
score	13.211869

Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia

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