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Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation

Background: Gel staining is a crucial step that allows the visualisation of proteins separated through SDS-PAGE. Col‑ loidal Coomassie Brilliant Blue-G (CBB-G) staining is among the commonly used visualisation methods due to several factors such as compatibility with mass spectrometry (MS) analysi...

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Main Authors: Nikson Chong, Fatt‑Ming, Hasnain, Hussain, Nur Ezzati, Hamdin, David Sheng Wee, Hong, Mehvish, Nisar, Jie Yan, Wei, Benjamin Chung Lau, Yii, Norasfaliza, Rahmad
Format: Article
Language:English
Published: BioMed Central Ltd 2022
Subjects:
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/38633/1/Higher%20resolution%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/38633/
https://chembioagro.springeropen.com/articles/10.1186/s40538-022-00297-0
https://doi.org/10.1186/s40538-022-00297-0
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spelling my.unimas.ir.386332022-06-13T03:12:03Z http://ir.unimas.my/id/eprint/38633/ Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation Nikson Chong, Fatt‑Ming Hasnain, Hussain Nur Ezzati, Hamdin David Sheng Wee, Hong Mehvish, Nisar Jie Yan, Wei Benjamin Chung Lau, Yii Norasfaliza, Rahmad S Agriculture (General) Background: Gel staining is a crucial step that allows the visualisation of proteins separated through SDS-PAGE. Col‑ loidal Coomassie Brilliant Blue-G (CBB-G) staining is among the commonly used visualisation methods due to several factors such as compatibility with mass spectrometry (MS) analysis, sensitivity, reproducibility, and simplicity of the staining process. However, the standard colloidal CBB-G staining has a drawback: the resolution of protein bands is compromised because of difusion of proteins during the washing step. Results: A modifcation to an established colloidal CBB-G staining method, which greatly increases the resolution of protein bands, is described. The addition of a fxation step, which prevents the difusion of proteins during the wash‑ ing step, is shown to increase protein band resolution. Conclusion: The fxation step is fast, fexible, and also retains all the advantages of the standard colloidal CBB-G stain‑ing methods. As there are no drawbacks, incorporating this fxation step into the standard colloidal CBB-G staining is an easy way to improve protein visualisation in SDS-PAGE. BioMed Central Ltd 2022 Article PeerReviewed text en http://ir.unimas.my/id/eprint/38633/1/Higher%20resolution%20-%20Copy.pdf Nikson Chong, Fatt‑Ming and Hasnain, Hussain and Nur Ezzati, Hamdin and David Sheng Wee, Hong and Mehvish, Nisar and Jie Yan, Wei and Benjamin Chung Lau, Yii and Norasfaliza, Rahmad (2022) Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation. Chemical and Biological Technologies in Agriculture, 9 (32). pp. 1-9. ISSN 2196-5641 https://chembioagro.springeropen.com/articles/10.1186/s40538-022-00297-0 https://doi.org/10.1186/s40538-022-00297-0
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Nikson Chong, Fatt‑Ming
Hasnain, Hussain
Nur Ezzati, Hamdin
David Sheng Wee, Hong
Mehvish, Nisar
Jie Yan, Wei
Benjamin Chung Lau, Yii
Norasfaliza, Rahmad
Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation
description Background: Gel staining is a crucial step that allows the visualisation of proteins separated through SDS-PAGE. Col‑ loidal Coomassie Brilliant Blue-G (CBB-G) staining is among the commonly used visualisation methods due to several factors such as compatibility with mass spectrometry (MS) analysis, sensitivity, reproducibility, and simplicity of the staining process. However, the standard colloidal CBB-G staining has a drawback: the resolution of protein bands is compromised because of difusion of proteins during the washing step. Results: A modifcation to an established colloidal CBB-G staining method, which greatly increases the resolution of protein bands, is described. The addition of a fxation step, which prevents the difusion of proteins during the wash‑ ing step, is shown to increase protein band resolution. Conclusion: The fxation step is fast, fexible, and also retains all the advantages of the standard colloidal CBB-G stain‑ing methods. As there are no drawbacks, incorporating this fxation step into the standard colloidal CBB-G staining is an easy way to improve protein visualisation in SDS-PAGE.
format Article
author Nikson Chong, Fatt‑Ming
Hasnain, Hussain
Nur Ezzati, Hamdin
David Sheng Wee, Hong
Mehvish, Nisar
Jie Yan, Wei
Benjamin Chung Lau, Yii
Norasfaliza, Rahmad
author_facet Nikson Chong, Fatt‑Ming
Hasnain, Hussain
Nur Ezzati, Hamdin
David Sheng Wee, Hong
Mehvish, Nisar
Jie Yan, Wei
Benjamin Chung Lau, Yii
Norasfaliza, Rahmad
author_sort Nikson Chong, Fatt‑Ming
title Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation
title_short Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation
title_full Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation
title_fullStr Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation
title_full_unstemmed Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fxation
title_sort higher resolution protein band visualisation via improvement of colloidal cbb-g staining by gel fxation
publisher BioMed Central Ltd
publishDate 2022
url http://ir.unimas.my/id/eprint/38633/1/Higher%20resolution%20-%20Copy.pdf
http://ir.unimas.my/id/eprint/38633/
https://chembioagro.springeropen.com/articles/10.1186/s40538-022-00297-0
https://doi.org/10.1186/s40538-022-00297-0
_version_ 1735573588002471936
score 13.211869

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