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Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm

Vibrio species is one of the pathogenic bacteria infecting shrimps in aquaculture farm has caused severe loss to the aquaculture farm. The aim of this research is to isolate and compare the presence of Vibrio spp. in commercialize shrimp farm during the stocking process and the final harvest pha...

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Main Authors: Elexson, Nillian, Nur Diyana, Zakaria, Dalene, Lesen, Nur Hafizah, Mohd Yusoff, Nurain Syahirah, Ismail, Teng, Sing Tung, Lesley Maurice, Bilung
Format: Article
Language:English
Published: 2022
Subjects:
QL Zoology
Online Access:http://ir.unimas.my/id/eprint/37928/1/Comparison%20distribution%20of%20Vibrio%20species.pdf
http://ir.unimas.my/id/eprint/37928/
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spelling my.unimas.ir.379282022-03-02T06:28:43Z http://ir.unimas.my/id/eprint/37928/ Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm Elexson, Nillian Nur Diyana, Zakaria Dalene, Lesen Nur Hafizah, Mohd Yusoff Nurain Syahirah, Ismail Teng, Sing Tung Lesley Maurice, Bilung QL Zoology Vibrio species is one of the pathogenic bacteria infecting shrimps in aquaculture farm has caused severe loss to the aquaculture farm. The aim of this research is to isolate and compare the presence of Vibrio spp. in commercialize shrimp farm during the stocking process and the final harvest phase of shrimp productions. Quantification and isolation were conducted and measured using Log CFU (colony forming units) counts and morphological investigation gram staining and conventional biochemical test identification. A total of sixty-four (n=64) sediment and water samples were collected from the inlet and outlet of the two selected shrimp ponds (Pond A and Pond B) in Kampung Telaga Air, Kuching, Sarawak. As a result, the bacteria count from water samples during stocking period at both shrimps ponnds were 6.6 Log CFU/ml and 5.6 Log CFU/ml while during harvesting period were 5.3 Log CFU/ml and 5.8 Log CFU/ml. Meanwhile, bacteria count sediments samples during stocking period at both shrimp ponds were 5.5 Log CFU/g and 5.7 Log CFU/g whereas harvesting period at showed both shrimps pond were 5.2 Log CFU/g and 4.8 Log CFU/g. The finding showed the Vibrio sp. identified in sediment samples were V. parahemolyticus with 27%, followed by V. alginolyticus (22%), V. cholerae (16%). V. fluvialis (14%) and 7% of V. fulmisi and V. vulnificus and V. mimicus. Meanwhile, the Vibrio sp. identified in water samples were V. fluvialis with 29%, followed by V. alginolyticus (22%), V. parahemolyticus (19%) and 10 % were V. fulmisi , V. mimicus and V. vulnificus. The study has been carried out further by investigated the Antibiotic Susceptibility Test (AST) using erythromycin (15 ug/ml), ampicillin (10 ug/ml), and streptomycin (25 ug/ml). The positive cultures from sediment samples showed Ampicillin resistant profile was > 0.2 MAR index while fifty percent (50%) of the isolates from water samples were resistant to ampicillin. Therefore, this study has profound implications for further monitoring process from the stocking process towards the harvesting process in order to prevent any Vibrio sp. infection which caused shrimp’s disease or food safety issue related to public consumption or of shrimps in future. 2022 Article PeerReviewed text en http://ir.unimas.my/id/eprint/37928/1/Comparison%20distribution%20of%20Vibrio%20species.pdf Elexson, Nillian and Nur Diyana, Zakaria and Dalene, Lesen and Nur Hafizah, Mohd Yusoff and Nurain Syahirah, Ismail and Teng, Sing Tung and Lesley Maurice, Bilung (2022) Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm. International Journal of Biology and Biomedical Engineering, 16. pp. 168-174. ISSN 1998-4510 10.46300/91011.2022.16.22
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QL Zoology
spellingShingle QL Zoology
Elexson, Nillian
Nur Diyana, Zakaria
Dalene, Lesen
Nur Hafizah, Mohd Yusoff
Nurain Syahirah, Ismail
Teng, Sing Tung
Lesley Maurice, Bilung
Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm
description Vibrio species is one of the pathogenic bacteria infecting shrimps in aquaculture farm has caused severe loss to the aquaculture farm. The aim of this research is to isolate and compare the presence of Vibrio spp. in commercialize shrimp farm during the stocking process and the final harvest phase of shrimp productions. Quantification and isolation were conducted and measured using Log CFU (colony forming units) counts and morphological investigation gram staining and conventional biochemical test identification. A total of sixty-four (n=64) sediment and water samples were collected from the inlet and outlet of the two selected shrimp ponds (Pond A and Pond B) in Kampung Telaga Air, Kuching, Sarawak. As a result, the bacteria count from water samples during stocking period at both shrimps ponnds were 6.6 Log CFU/ml and 5.6 Log CFU/ml while during harvesting period were 5.3 Log CFU/ml and 5.8 Log CFU/ml. Meanwhile, bacteria count sediments samples during stocking period at both shrimp ponds were 5.5 Log CFU/g and 5.7 Log CFU/g whereas harvesting period at showed both shrimps pond were 5.2 Log CFU/g and 4.8 Log CFU/g. The finding showed the Vibrio sp. identified in sediment samples were V. parahemolyticus with 27%, followed by V. alginolyticus (22%), V. cholerae (16%). V. fluvialis (14%) and 7% of V. fulmisi and V. vulnificus and V. mimicus. Meanwhile, the Vibrio sp. identified in water samples were V. fluvialis with 29%, followed by V. alginolyticus (22%), V. parahemolyticus (19%) and 10 % were V. fulmisi , V. mimicus and V. vulnificus. The study has been carried out further by investigated the Antibiotic Susceptibility Test (AST) using erythromycin (15 ug/ml), ampicillin (10 ug/ml), and streptomycin (25 ug/ml). The positive cultures from sediment samples showed Ampicillin resistant profile was > 0.2 MAR index while fifty percent (50%) of the isolates from water samples were resistant to ampicillin. Therefore, this study has profound implications for further monitoring process from the stocking process towards the harvesting process in order to prevent any Vibrio sp. infection which caused shrimp’s disease or food safety issue related to public consumption or of shrimps in future.
format Article
author Elexson, Nillian
Nur Diyana, Zakaria
Dalene, Lesen
Nur Hafizah, Mohd Yusoff
Nurain Syahirah, Ismail
Teng, Sing Tung
Lesley Maurice, Bilung
author_facet Elexson, Nillian
Nur Diyana, Zakaria
Dalene, Lesen
Nur Hafizah, Mohd Yusoff
Nurain Syahirah, Ismail
Teng, Sing Tung
Lesley Maurice, Bilung
author_sort Elexson, Nillian
title Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm
title_short Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm
title_full Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm
title_fullStr Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm
title_full_unstemmed Comparison distribution of Vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm
title_sort comparison distribution of vibrio species in stocking to harvesting process of shrimp at commercialize shrimp farm
publishDate 2022
url http://ir.unimas.my/id/eprint/37928/1/Comparison%20distribution%20of%20Vibrio%20species.pdf
http://ir.unimas.my/id/eprint/37928/
_version_ 1726796158949392384
score 13.211869

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