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Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio)

Dially I phthalate (DAP) and dipropy I phthalate (DPP) belong to a group of chemicals called phthalate esters (P AEs) which are commonly used as plasticisers. However, higher temperature of the environment is able to break the weak bond of the weak bonding between these chemical with polyvinyl chlor...

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Main Author: Mohamad Shamil Faris, Mohamad Khalid
Format: Thesis
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
Subjects:
QP Physiology
Online Access:http://ir.unimas.my/id/eprint/37887/1/Mohamad%20Shamil%20Faris%20Bin%20Mohamad%20Khalid%2024pgs.pdf
http://ir.unimas.my/id/eprint/37887/4/Mohamad%20Shamil%20Faris%20Bin%20Mohamad%20Khalid%20ft.pdf
http://ir.unimas.my/id/eprint/37887/
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spelling my.unimas.ir.378872023-03-23T08:06:45Z http://ir.unimas.my/id/eprint/37887/ Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio) Mohamad Shamil Faris, Mohamad Khalid QP Physiology Dially I phthalate (DAP) and dipropy I phthalate (DPP) belong to a group of chemicals called phthalate esters (P AEs) which are commonly used as plasticisers. However, higher temperature of the environment is able to break the weak bond of the weak bonding between these chemical with polyvinyl chloride (PVC) allow it to leak into the environment. These two chemical was listed as carcinogenicity and endocrine disrupting potentials. This study was set out to study the LCso and the morphological d feet of DAP, DPP and DPP+DAP chemical on zebrafish embryonic development. Toxicity of the chemicals is determined by using pro bit analysis. The LC50 are measured at 96 hpf. Morphological defect sllch as unhatched, pericardia edema, yolk sac edema, uninflated swim bladder and tail defonnation, which were measured at 96 hpf. The LCso was determined to be at 6.58 mg/I for DPP, 5.12 mg/I for DAP and 1.71 mg/I for 0 P+DPP with 95% confidence level. The LClO was determined to be at 3.80 mg/I for DPP, 3.60 mgll for DAP, 0.92 mg/l for DAP + DPP with 95% confidence level. The thyroid hormone (TH) gene marker used in this study are; diodinase I(dio_l), diodinase 2(dio_2), Tli receptor alpha (Iro), TH receptor beta (trfJ), thyroid stimulating honnone beta (tshjJ) and transthyreiin (ttr). This study demonstrated that DPP, DAP, DPP+DAP indllced developmental toxicity, impaired the structure and functions of developing organ, and altered the expression of the thyroid hormone gene marker. Universiti Malaysia Sarawak, (UNIMAS) 2018 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/37887/1/Mohamad%20Shamil%20Faris%20Bin%20Mohamad%20Khalid%2024pgs.pdf text en http://ir.unimas.my/id/eprint/37887/4/Mohamad%20Shamil%20Faris%20Bin%20Mohamad%20Khalid%20ft.pdf Mohamad Shamil Faris, Mohamad Khalid (2018) Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio). Masters thesis, Universiti Malaysia Sarawak, (UNIMAS).
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic QP Physiology
spellingShingle QP Physiology
Mohamad Shamil Faris, Mohamad Khalid
Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio)
description Dially I phthalate (DAP) and dipropy I phthalate (DPP) belong to a group of chemicals called phthalate esters (P AEs) which are commonly used as plasticisers. However, higher temperature of the environment is able to break the weak bond of the weak bonding between these chemical with polyvinyl chloride (PVC) allow it to leak into the environment. These two chemical was listed as carcinogenicity and endocrine disrupting potentials. This study was set out to study the LCso and the morphological d feet of DAP, DPP and DPP+DAP chemical on zebrafish embryonic development. Toxicity of the chemicals is determined by using pro bit analysis. The LC50 are measured at 96 hpf. Morphological defect sllch as unhatched, pericardia edema, yolk sac edema, uninflated swim bladder and tail defonnation, which were measured at 96 hpf. The LCso was determined to be at 6.58 mg/I for DPP, 5.12 mg/I for DAP and 1.71 mg/I for 0 P+DPP with 95% confidence level. The LClO was determined to be at 3.80 mg/I for DPP, 3.60 mgll for DAP, 0.92 mg/l for DAP + DPP with 95% confidence level. The thyroid hormone (TH) gene marker used in this study are; diodinase I(dio_l), diodinase 2(dio_2), Tli receptor alpha (Iro), TH receptor beta (trfJ), thyroid stimulating honnone beta (tshjJ) and transthyreiin (ttr). This study demonstrated that DPP, DAP, DPP+DAP indllced developmental toxicity, impaired the structure and functions of developing organ, and altered the expression of the thyroid hormone gene marker.
format Thesis
author Mohamad Shamil Faris, Mohamad Khalid
author_facet Mohamad Shamil Faris, Mohamad Khalid
author_sort Mohamad Shamil Faris, Mohamad Khalid
title Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio)
title_short Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio)
title_full Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio)
title_fullStr Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio)
title_full_unstemmed Effect of Toxicity of Phthalate Esters during Early Vertebrate Development of Zebrafish (Danio rerio)
title_sort effect of toxicity of phthalate esters during early vertebrate development of zebrafish (danio rerio)
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/37887/1/Mohamad%20Shamil%20Faris%20Bin%20Mohamad%20Khalid%2024pgs.pdf
http://ir.unimas.my/id/eprint/37887/4/Mohamad%20Shamil%20Faris%20Bin%20Mohamad%20Khalid%20ft.pdf
http://ir.unimas.my/id/eprint/37887/
_version_ 1761623578866876416
score 13.211869

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