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Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture

This study was conducted to compare the agronomic performance of four elite cocoa clones (MCBC1, KKM22, KKM4 and PBC230) regenerated from staminode and immature zygotic embryo culture with conventional grafted cocoa clones. From the results, it was found that the KKM4 clone propagated...

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Main Authors: Gibson, Entuni, Hollena, Nori, Rebicca, Edward, Ahmad Kamil, Bin Mohd Jaafar
Format: Article
Language:English
Published: King Mongkut's Institute of Technology Ladkrabang (KMITL) 2021
Subjects:
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/37640/1/agronomic1.pdf
http://ir.unimas.my/id/eprint/37640/
https://li01.tci-thaijo.org/index.php/cast/issue/view/17151
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spelling my.unimas.ir.376402022-01-05T01:12:59Z http://ir.unimas.my/id/eprint/37640/ Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture Gibson, Entuni Hollena, Nori Rebicca, Edward Ahmad Kamil, Bin Mohd Jaafar S Agriculture (General) This study was conducted to compare the agronomic performance of four elite cocoa clones (MCBC1, KKM22, KKM4 and PBC230) regenerated from staminode and immature zygotic embryo culture with conventional grafted cocoa clones. From the results, it was found that the KKM4 clone propagated from immature zygotic embryo culture exhibited variations in the fresh pod weight (339.6 g), fresh individual seed weight (4.13 g) and number of flat beans per pod (4 beans) compared with the rest of the regenerated clones. The genetic stability of the somatic embryogenesis cultured clones and the donor clones was then tested using fragment analysis with five SSR primers, i.e. mTcCIR7, mTcCIR18, mTcCIR22, mTcCIR33 and mTcCIR40. Four of these primers identified variations in the allele size and allele addition in KKM4 clone from immature zygotic embryo. Molecular analysis validated that the difference in agronomic performance of the KKM4 clone from immature zygotic embryo culture was due to genetic mutation created during the immature zygotic embryo culture process. King Mongkut's Institute of Technology Ladkrabang (KMITL) 2021-05-18 Article PeerReviewed text en http://ir.unimas.my/id/eprint/37640/1/agronomic1.pdf Gibson, Entuni and Hollena, Nori and Rebicca, Edward and Ahmad Kamil, Bin Mohd Jaafar (2021) Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture. Current Applied Science and Technology, 22 (1). pp. 1-10. ISSN 2586-9396 https://li01.tci-thaijo.org/index.php/cast/issue/view/17151
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Gibson, Entuni
Hollena, Nori
Rebicca, Edward
Ahmad Kamil, Bin Mohd Jaafar
Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture
description This study was conducted to compare the agronomic performance of four elite cocoa clones (MCBC1, KKM22, KKM4 and PBC230) regenerated from staminode and immature zygotic embryo culture with conventional grafted cocoa clones. From the results, it was found that the KKM4 clone propagated from immature zygotic embryo culture exhibited variations in the fresh pod weight (339.6 g), fresh individual seed weight (4.13 g) and number of flat beans per pod (4 beans) compared with the rest of the regenerated clones. The genetic stability of the somatic embryogenesis cultured clones and the donor clones was then tested using fragment analysis with five SSR primers, i.e. mTcCIR7, mTcCIR18, mTcCIR22, mTcCIR33 and mTcCIR40. Four of these primers identified variations in the allele size and allele addition in KKM4 clone from immature zygotic embryo. Molecular analysis validated that the difference in agronomic performance of the KKM4 clone from immature zygotic embryo culture was due to genetic mutation created during the immature zygotic embryo culture process.
format Article
author Gibson, Entuni
Hollena, Nori
Rebicca, Edward
Ahmad Kamil, Bin Mohd Jaafar
author_facet Gibson, Entuni
Hollena, Nori
Rebicca, Edward
Ahmad Kamil, Bin Mohd Jaafar
author_sort Gibson, Entuni
title Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture
title_short Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture
title_full Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture
title_fullStr Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture
title_full_unstemmed Agronomic Performance and Genetic Fidelity of the Selected Elite Cocoa Clones Derived from Somatic Embryogenesis Culture
title_sort agronomic performance and genetic fidelity of the selected elite cocoa clones derived from somatic embryogenesis culture
publisher King Mongkut's Institute of Technology Ladkrabang (KMITL)
publishDate 2021
url http://ir.unimas.my/id/eprint/37640/1/agronomic1.pdf
http://ir.unimas.my/id/eprint/37640/
https://li01.tci-thaijo.org/index.php/cast/issue/view/17151
_version_ 1724078543033335808
score 13.211869

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