Staff View: Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo

Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo

Objective: Coronaviruses (CoVs) are natural commensals of bats. Two subgenera, namely Sarbecoviruses and Merbecoviruses have a high zoonotic potential and have been associated with three separate spillover events in the past 2 decades, making surveillance of bat-CoVs crucial for the prevention of...

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Main Authors:	Cheng Siang, Tan, Vaenessa, Noni, Jaya Seelan, Sathiya Seelan, Azroie, Denel, Faisal Ali, Anwarali Khan
Format:	Article
Language:	English
Published:	Biomed Central 2021
Subjects:	QL Zoology QR355 Virology
Online Access:	http://ir.unimas.my/id/eprint/37420/1/coronavirus1.pdf http://ir.unimas.my/id/eprint/37420/ https://bmcresnotes.biomedcentral.com/ https://doi.org/10.1186/s13104-021-05880-6
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spelling	my.unimas.ir.374202021-12-20T23:46:01Z http://ir.unimas.my/id/eprint/37420/ Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo Cheng Siang, Tan Vaenessa, Noni Jaya Seelan, Sathiya Seelan Azroie, Denel Faisal Ali, Anwarali Khan QL Zoology QR355 Virology Objective: Coronaviruses (CoVs) are natural commensals of bats. Two subgenera, namely Sarbecoviruses and Merbecoviruses have a high zoonotic potential and have been associated with three separate spillover events in the past 2 decades, making surveillance of bat-CoVs crucial for the prevention of the next epidemic. The study was aimed to elucidate the presence of coronavirus in fresh bat guano sampled from Wind Cave Nature Reserve (WCNR) in Sarawak, Malaysian Borneo. Samples collected were placed into viral transport medium, transported on ice within the collection day, and preserved at − 80 °C. Nucleic acid was extracted using the column method and screened using consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene. Amplicons were sequenced bidi‑ rectionally using the Sanger method. Phylogenetic tree with maximum-likelihood bootstrap and Bayesian posterior probability were constructed. Results: CoV-RNA was detected in ten specimens (47.6%, n =21). Six alphacoronavirus and four betacoronaviruses were identified. The bat-CoVs can be phylogenetically grouped into four novel clades which are closely related to Decacovirus-1 and Decacovirus-2, Sarbecovirus, and an unclassified CoV. CoVs lineages unique to the Island of Borneo were discovered in Sarawak, Malaysia, with one of them closely related to Sarbecovirus. All of them are distant from currently known human coronaviruses. Keywords: Bat, Cave, Coronavirus, Guano, Sarawak Biomed Central 2021-12-20 Article PeerReviewed text en http://ir.unimas.my/id/eprint/37420/1/coronavirus1.pdf Cheng Siang, Tan and Vaenessa, Noni and Jaya Seelan, Sathiya Seelan and Azroie, Denel and Faisal Ali, Anwarali Khan (2021) Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo. BMC Research Notes, 14 (461). pp. 1-6. ISSN 1756-0500 https://bmcresnotes.biomedcentral.com/ https://doi.org/10.1186/s13104-021-05880-6
institution	Universiti Malaysia Sarawak
building	Centre for Academic Information Services (CAIS)
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Sarawak
content_source	UNIMAS Institutional Repository
url_provider	http://ir.unimas.my/
language	English
topic	QL Zoology QR355 Virology
spellingShingle	QL Zoology QR355 Virology Cheng Siang, Tan Vaenessa, Noni Jaya Seelan, Sathiya Seelan Azroie, Denel Faisal Ali, Anwarali Khan Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
description	Objective: Coronaviruses (CoVs) are natural commensals of bats. Two subgenera, namely Sarbecoviruses and Merbecoviruses have a high zoonotic potential and have been associated with three separate spillover events in the past 2 decades, making surveillance of bat-CoVs crucial for the prevention of the next epidemic. The study was aimed to elucidate the presence of coronavirus in fresh bat guano sampled from Wind Cave Nature Reserve (WCNR) in Sarawak, Malaysian Borneo. Samples collected were placed into viral transport medium, transported on ice within the collection day, and preserved at − 80 °C. Nucleic acid was extracted using the column method and screened using consensus PCR primers targeting the RNA-dependent RNA polymerase (RdRp) gene. Amplicons were sequenced bidi‑ rectionally using the Sanger method. Phylogenetic tree with maximum-likelihood bootstrap and Bayesian posterior probability were constructed. Results: CoV-RNA was detected in ten specimens (47.6%, n =21). Six alphacoronavirus and four betacoronaviruses were identified. The bat-CoVs can be phylogenetically grouped into four novel clades which are closely related to Decacovirus-1 and Decacovirus-2, Sarbecovirus, and an unclassified CoV. CoVs lineages unique to the Island of Borneo were discovered in Sarawak, Malaysia, with one of them closely related to Sarbecovirus. All of them are distant from currently known human coronaviruses. Keywords: Bat, Cave, Coronavirus, Guano, Sarawak
format	Article
author	Cheng Siang, Tan Vaenessa, Noni Jaya Seelan, Sathiya Seelan Azroie, Denel Faisal Ali, Anwarali Khan
author_facet	Cheng Siang, Tan Vaenessa, Noni Jaya Seelan, Sathiya Seelan Azroie, Denel Faisal Ali, Anwarali Khan
author_sort	Cheng Siang, Tan
title	Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_short	Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_full	Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_fullStr	Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_full_unstemmed	Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo
title_sort	ecological surveillance of bat coronaviruses in sarawak, malaysian borneo
publisher	Biomed Central
publishDate	2021
url	http://ir.unimas.my/id/eprint/37420/1/coronavirus1.pdf http://ir.unimas.my/id/eprint/37420/ https://bmcresnotes.biomedcentral.com/ https://doi.org/10.1186/s13104-021-05880-6
_version_	1720440452799266816
score	13.211869

Ecological surveillance of bat coronaviruses in Sarawak, Malaysian Borneo

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