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Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains

Leptospira spp. cause leptospirosis in human with vary signs from asymptomatic, mild to severe and even death. Leptospira can be classified into pathogenic, intermediate and saprophytic strains. Leptospirosis treatment relies on data related to leptospiral susceptibility to different antibiotics. A...

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Main Author: Stella, Ngu Pei Ling
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/36420/1/Stella%20Ngu%20ft.pdf
http://ir.unimas.my/id/eprint/36420/
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spelling my.unimas.ir.364202024-08-14T06:47:42Z http://ir.unimas.my/id/eprint/36420/ Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains Stella, Ngu Pei Ling Q Science (General) Leptospira spp. cause leptospirosis in human with vary signs from asymptomatic, mild to severe and even death. Leptospira can be classified into pathogenic, intermediate and saprophytic strains. Leptospirosis treatment relies on data related to leptospiral susceptibility to different antibiotics. Antimicrobial susceptibility testing (AST) is generally performed in clinical microbiology laboratory to assess the antimicrobial activities of antibiotics to bacteria. However, the recent availability of AST for Leptospira and sensitivity of intermediate and saprophytic strains to antibiotics are still not well developed. This study concerns the antibiotic susceptibility determination in intermediate and saprophytic strains. Ten intermediate and 10 saprophytic strains were assessed against six antibiotics through broth microdilution method. Serial two-fold dilution of antibiotic was added into Ellinghausen-McCullough-Johnson-Harris (EMJH) broth, then inoculated and incubated overnight at 30 °C in dark conditions. OD42o readings of microplate reader were used to determine Minimal Inhibitory Concentration (MIC50) values. Ciprofloxacin, chloramphenicol, and doxycycline had lower MIC5os than penicillin G, amoxicillin, and cefotaxime. Amoxicillin had the highest MIC50 value, with more than 12 800 μg/mL. The results of this study will provide a baseline information on the AST among Leptospira spp. in Malaysia and worldwide. Universiti Malaysia Sarawak, (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/36420/1/Stella%20Ngu%20ft.pdf Stella, Ngu Pei Ling (2018) Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Stella, Ngu Pei Ling
Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains
description Leptospira spp. cause leptospirosis in human with vary signs from asymptomatic, mild to severe and even death. Leptospira can be classified into pathogenic, intermediate and saprophytic strains. Leptospirosis treatment relies on data related to leptospiral susceptibility to different antibiotics. Antimicrobial susceptibility testing (AST) is generally performed in clinical microbiology laboratory to assess the antimicrobial activities of antibiotics to bacteria. However, the recent availability of AST for Leptospira and sensitivity of intermediate and saprophytic strains to antibiotics are still not well developed. This study concerns the antibiotic susceptibility determination in intermediate and saprophytic strains. Ten intermediate and 10 saprophytic strains were assessed against six antibiotics through broth microdilution method. Serial two-fold dilution of antibiotic was added into Ellinghausen-McCullough-Johnson-Harris (EMJH) broth, then inoculated and incubated overnight at 30 °C in dark conditions. OD42o readings of microplate reader were used to determine Minimal Inhibitory Concentration (MIC50) values. Ciprofloxacin, chloramphenicol, and doxycycline had lower MIC5os than penicillin G, amoxicillin, and cefotaxime. Amoxicillin had the highest MIC50 value, with more than 12 800 μg/mL. The results of this study will provide a baseline information on the AST among Leptospira spp. in Malaysia and worldwide.
format Final Year Project Report
author Stella, Ngu Pei Ling
author_facet Stella, Ngu Pei Ling
author_sort Stella, Ngu Pei Ling
title Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains
title_short Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains
title_full Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains
title_fullStr Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains
title_full_unstemmed Study of Antibiotic Susceptibility of Intermediate and Saprophytic Leptospira Strains
title_sort study of antibiotic susceptibility of intermediate and saprophytic leptospira strains
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/36420/1/Stella%20Ngu%20ft.pdf
http://ir.unimas.my/id/eprint/36420/
_version_ 1808981488267427840
score 13.211869

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