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Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan)

Overpopulation and increase in demand of timer have caused the depletion of timber resources. Therefore, Neolamarckia macrophylla (red kelampayan) belongs to family Rubiaceae is selected as one of the species for forest plantation and reforestation programmes in Sarawak due to its fast growing char...

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Main Author: Chia, Chun Min
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
Subjects:
QK Botany
Online Access:http://ir.unimas.my/id/eprint/36411/1/CHIA%20CHUN%20MIN%20%2824%20pgs%29.pdf
http://ir.unimas.my/id/eprint/36411/4/CHIA%20CHUN%20ft.pdf
http://ir.unimas.my/id/eprint/36411/
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spelling my.unimas.ir.364112023-03-03T01:58:35Z http://ir.unimas.my/id/eprint/36411/ Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan) Chia, Chun Min QK Botany Overpopulation and increase in demand of timer have caused the depletion of timber resources. Therefore, Neolamarckia macrophylla (red kelampayan) belongs to family Rubiaceae is selected as one of the species for forest plantation and reforestation programmes in Sarawak due to its fast growing characteristic. However, conventional propagation of N. macrophylla is time consuming and possessed several problems mentioned. Therefore, in vitro shoot induction of N. macrophylla should be explored for mass propagation purposes in order to meet the global demands for timber resources. Effect of BAP on in vitro shoot induction of N. macrophylla was investigated. The optimum concentration of BAP on in vitro shoot induction of N. macrophylla was determined. In this study, B5 media supplemented with different concentration of BAP (0.5, 1.0 and 1.5 mg/L) was used for in vitro shoot induction from nodal explants of N. macrophylla. The parameters used in this study were number of shoots, shoot regeneration percentage (%), multiple shoot induction percentage (%) and shoot length (cm). The data was analyzed by using One-way ANOVA and Duncan's Multiple Range Test (DMRT) in SPSSS version 22. Result showed that the B5 supplemented with 0.5 mg/L BAP had given the highest mean number of shoots with 3.80 shoots per explant. 100% of shoot regeneration was observed in B5 treated with 0.5 and 1.0 mg/L while highest shoot length was observed in B5 blank. One-way ANOVA showed significant effect of BAP in terms of number of shoots regenerated. In this study, B5 supplemented with 0.5 mg/L BAP was identified as optimum concentration on in vitro shoot induction of N. macrophylla. Universiti Malaysia Sarawak, (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/36411/1/CHIA%20CHUN%20MIN%20%2824%20pgs%29.pdf text en http://ir.unimas.my/id/eprint/36411/4/CHIA%20CHUN%20ft.pdf Chia, Chun Min (2018) Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan). [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic QK Botany
spellingShingle QK Botany
Chia, Chun Min
Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan)
description Overpopulation and increase in demand of timer have caused the depletion of timber resources. Therefore, Neolamarckia macrophylla (red kelampayan) belongs to family Rubiaceae is selected as one of the species for forest plantation and reforestation programmes in Sarawak due to its fast growing characteristic. However, conventional propagation of N. macrophylla is time consuming and possessed several problems mentioned. Therefore, in vitro shoot induction of N. macrophylla should be explored for mass propagation purposes in order to meet the global demands for timber resources. Effect of BAP on in vitro shoot induction of N. macrophylla was investigated. The optimum concentration of BAP on in vitro shoot induction of N. macrophylla was determined. In this study, B5 media supplemented with different concentration of BAP (0.5, 1.0 and 1.5 mg/L) was used for in vitro shoot induction from nodal explants of N. macrophylla. The parameters used in this study were number of shoots, shoot regeneration percentage (%), multiple shoot induction percentage (%) and shoot length (cm). The data was analyzed by using One-way ANOVA and Duncan's Multiple Range Test (DMRT) in SPSSS version 22. Result showed that the B5 supplemented with 0.5 mg/L BAP had given the highest mean number of shoots with 3.80 shoots per explant. 100% of shoot regeneration was observed in B5 treated with 0.5 and 1.0 mg/L while highest shoot length was observed in B5 blank. One-way ANOVA showed significant effect of BAP in terms of number of shoots regenerated. In this study, B5 supplemented with 0.5 mg/L BAP was identified as optimum concentration on in vitro shoot induction of N. macrophylla.
format Final Year Project Report
author Chia, Chun Min
author_facet Chia, Chun Min
author_sort Chia, Chun Min
title Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan)
title_short Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan)
title_full Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan)
title_fullStr Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan)
title_full_unstemmed Effect of BAP on In Vitro Shoot Induction of Neolamarckia macrophylla (Red Kelampayan)
title_sort effect of bap on in vitro shoot induction of neolamarckia macrophylla (red kelampayan)
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/36411/1/CHIA%20CHUN%20MIN%20%2824%20pgs%29.pdf
http://ir.unimas.my/id/eprint/36411/4/CHIA%20CHUN%20ft.pdf
http://ir.unimas.my/id/eprint/36411/
_version_ 1759693324670730240
score 13.211869

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