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Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson

Comparative anatomical studies were carried out on in vivo plants of Triphasia trifolia (Burm. f.) P. Wilson and in vitro plantlets of the same age. To get the in vitro plantlets, explants were cultured on MS (Murashige and Skoog) media supplemented with different concentrations and combinations...

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Main Authors: S., Abdullah, R.M., Taha, N.A., Hasbullah, N., Mohamed, Hashimah, Elias, N.W., Haron
Format: Article
Language:English
Published: ISHS 2013
Subjects:
S Agriculture (General)
SB Plant culture
Online Access:http://ir.unimas.my/id/eprint/36210/1/trifolia1.pdf
http://ir.unimas.my/id/eprint/36210/
http://www.actahort.org/books/975/975_11.htm
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spelling my.unimas.ir.362102021-09-28T02:33:00Z http://ir.unimas.my/id/eprint/36210/ Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson S., Abdullah R.M., Taha N.A., Hasbullah N., Mohamed Hashimah, Elias N.W., Haron S Agriculture (General) SB Plant culture Comparative anatomical studies were carried out on in vivo plants of Triphasia trifolia (Burm. f.) P. Wilson and in vitro plantlets of the same age. To get the in vitro plantlets, explants were cultured on MS (Murashige and Skoog) media supplemented with different concentrations and combinations of hormones. The explant sources of Triphasia trifolia (Burm. f.) P. Wilson were obtained from cotyledons, leaves, stems, roots and shoot tips that were placed under conditions of 16 hours light and 8 hours dark. The optimum media for regeneration was MS supplemented with 1.0 mg/L BAP and 1.0 mg/L NAA. Cotyledon explants were found to be the most responsive. Regeneration of complete plantlets was achieved from cotyledon explants after about 4 months in culture. Sectioning was done to study the characteristics of the respective vascular bundles, shape of cells, palisade cell layers, presence of oil glands, druse and cuticle layers. Vascular bundles of in vivo leaves were extremely well developed compared to those in in vitro leaves. The vascular bundle of the in vivo leaf showed well developed xylem. However, the xylem and phloem cells of the in vitro leaf were very poorly developed which is one of the features of in vitro plants. Scanning electron microscope (SEM) studies were also carried out on the in vivo and in vitro plantlets to observe differences on the leaf surface. ISHS 2013 Article PeerReviewed text en http://ir.unimas.my/id/eprint/36210/1/trifolia1.pdf S., Abdullah and R.M., Taha and N.A., Hasbullah and N., Mohamed and Hashimah, Elias and N.W., Haron (2013) Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson. Acta Horticulturae, 975 (11). pp. 111-118. ISSN 0567-7572 (print) 2406-6168 (electronic) http://www.actahort.org/books/975/975_11.htm 10.17660/ActaHortic.2013.975.11
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
SB Plant culture
spellingShingle S Agriculture (General)
SB Plant culture
S., Abdullah
R.M., Taha
N.A., Hasbullah
N., Mohamed
Hashimah, Elias
N.W., Haron
Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson
description Comparative anatomical studies were carried out on in vivo plants of Triphasia trifolia (Burm. f.) P. Wilson and in vitro plantlets of the same age. To get the in vitro plantlets, explants were cultured on MS (Murashige and Skoog) media supplemented with different concentrations and combinations of hormones. The explant sources of Triphasia trifolia (Burm. f.) P. Wilson were obtained from cotyledons, leaves, stems, roots and shoot tips that were placed under conditions of 16 hours light and 8 hours dark. The optimum media for regeneration was MS supplemented with 1.0 mg/L BAP and 1.0 mg/L NAA. Cotyledon explants were found to be the most responsive. Regeneration of complete plantlets was achieved from cotyledon explants after about 4 months in culture. Sectioning was done to study the characteristics of the respective vascular bundles, shape of cells, palisade cell layers, presence of oil glands, druse and cuticle layers. Vascular bundles of in vivo leaves were extremely well developed compared to those in in vitro leaves. The vascular bundle of the in vivo leaf showed well developed xylem. However, the xylem and phloem cells of the in vitro leaf were very poorly developed which is one of the features of in vitro plants. Scanning electron microscope (SEM) studies were also carried out on the in vivo and in vitro plantlets to observe differences on the leaf surface.
format Article
author S., Abdullah
R.M., Taha
N.A., Hasbullah
N., Mohamed
Hashimah, Elias
N.W., Haron
author_facet S., Abdullah
R.M., Taha
N.A., Hasbullah
N., Mohamed
Hashimah, Elias
N.W., Haron
author_sort S., Abdullah
title Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson
title_short Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson
title_full Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson
title_fullStr Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson
title_full_unstemmed Tissue Culture, Anatomical and Morphological Studies of Triphasia trifolia (Burm. f.) P. Wilson
title_sort tissue culture, anatomical and morphological studies of triphasia trifolia (burm. f.) p. wilson
publisher ISHS
publishDate 2013
url http://ir.unimas.my/id/eprint/36210/1/trifolia1.pdf
http://ir.unimas.my/id/eprint/36210/
http://www.actahort.org/books/975/975_11.htm
_version_ 1712289139195379712
score 13.211869

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