Cover Image

Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia

In this study, we examined the genetic structure of Tor tambroides Valenciennes, an important indigenous freshwater fish species in Malaysia, using sequence analysis of 464 base pairs of the mitochondrial cytochrome c oxidase I (COI) gene. In total, 92 T. tambroides samples were analyzed from 4 loca...

Full description

Saved in:
Bibliographic Details
Main Authors: Yuzine, Esa, Siti Shapor, Siraj, Siti Khalijah, Daud, Khairul Adha A., Rahim, Jeffrine Japning, Rovie-Ryan, Soon Guan, Tan
Format: Article
Language:English
Published: Biodiversity Research Center, Academia Sinica 2008
Subjects:
QL Zoology
Online Access:http://ir.unimas.my/id/eprint/36019/1/natural1.pdf
http://ir.unimas.my/id/eprint/36019/
https://zoolstud.sinica.edu.tw/473.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.36019
record_format eprints
spelling my.unimas.ir.360192021-09-06T06:54:30Z http://ir.unimas.my/id/eprint/36019/ Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia Yuzine, Esa Siti Shapor, Siraj Siti Khalijah, Daud Khairul Adha A., Rahim Jeffrine Japning, Rovie-Ryan Soon Guan, Tan QL Zoology In this study, we examined the genetic structure of Tor tambroides Valenciennes, an important indigenous freshwater fish species in Malaysia, using sequence analysis of 464 base pairs of the mitochondrial cytochrome c oxidase I (COI) gene. In total, 92 T. tambroides samples were analyzed from 4 locations on Peninsular Malaysia (n=87) and a single population from Sarawak (Batang Ai, n=5) on Borneo I., and 4 sequences of Tor douronensis from Sarawak were used for comparisons. In total, 9 haplotypes were found, with 7 haplotypes being unique and 2 haplotypes being shared among the 5 populations. The phylogenetic analysis using Neighbor-joining (NJ) and maximum-parsimony (MP) methods supported the monophyletic status between T. tambroides and T. douronensis, thus suggesting their status as different species. The clustering of all T. tambroides samples into a single clade suggested that their genetic identity belongs to a single species. The sharing of haplotype HKE4 between Batang Ai of Sarawak (n=4) and Perak of Peninsular Malaysia (n=3) reflects the historical connection of drainages between the regions possibly during Pleistocene glaciation periods. Limited variations were found among all peninsular T. tambroides populations. The low level of mitochondrial (mt) DNA differences currently found among T. tambroides populations is probably due to the high proportion of the HKE1 haplotype being found in all the populations (0.736-1.000), or the small number of samples used in the present study. Overall, the present study was able to shed light on the phylogenetic relationships and genetic structure of T. tambroides in Malaysia. Biodiversity Research Center, Academia Sinica 2008 Article PeerReviewed text en http://ir.unimas.my/id/eprint/36019/1/natural1.pdf Yuzine, Esa and Siti Shapor, Siraj and Siti Khalijah, Daud and Khairul Adha A., Rahim and Jeffrine Japning, Rovie-Ryan and Soon Guan, Tan (2008) Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia. Zoological Studies, 47 (3). pp. 360-367. ISSN 1810-522X https://zoolstud.sinica.edu.tw/473.html
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QL Zoology
spellingShingle QL Zoology
Yuzine, Esa
Siti Shapor, Siraj
Siti Khalijah, Daud
Khairul Adha A., Rahim
Jeffrine Japning, Rovie-Ryan
Soon Guan, Tan
Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia
description In this study, we examined the genetic structure of Tor tambroides Valenciennes, an important indigenous freshwater fish species in Malaysia, using sequence analysis of 464 base pairs of the mitochondrial cytochrome c oxidase I (COI) gene. In total, 92 T. tambroides samples were analyzed from 4 locations on Peninsular Malaysia (n=87) and a single population from Sarawak (Batang Ai, n=5) on Borneo I., and 4 sequences of Tor douronensis from Sarawak were used for comparisons. In total, 9 haplotypes were found, with 7 haplotypes being unique and 2 haplotypes being shared among the 5 populations. The phylogenetic analysis using Neighbor-joining (NJ) and maximum-parsimony (MP) methods supported the monophyletic status between T. tambroides and T. douronensis, thus suggesting their status as different species. The clustering of all T. tambroides samples into a single clade suggested that their genetic identity belongs to a single species. The sharing of haplotype HKE4 between Batang Ai of Sarawak (n=4) and Perak of Peninsular Malaysia (n=3) reflects the historical connection of drainages between the regions possibly during Pleistocene glaciation periods. Limited variations were found among all peninsular T. tambroides populations. The low level of mitochondrial (mt) DNA differences currently found among T. tambroides populations is probably due to the high proportion of the HKE1 haplotype being found in all the populations (0.736-1.000), or the small number of samples used in the present study. Overall, the present study was able to shed light on the phylogenetic relationships and genetic structure of T. tambroides in Malaysia.
format Article
author Yuzine, Esa
Siti Shapor, Siraj
Siti Khalijah, Daud
Khairul Adha A., Rahim
Jeffrine Japning, Rovie-Ryan
Soon Guan, Tan
author_facet Yuzine, Esa
Siti Shapor, Siraj
Siti Khalijah, Daud
Khairul Adha A., Rahim
Jeffrine Japning, Rovie-Ryan
Soon Guan, Tan
author_sort Yuzine, Esa
title Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia
title_short Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia
title_full Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia
title_fullStr Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia
title_full_unstemmed Mitochondrial DNA Diversity of Tor tambroides Valenciennes (Cyprinidae) from Five Natural Populations in Malaysia
title_sort mitochondrial dna diversity of tor tambroides valenciennes (cyprinidae) from five natural populations in malaysia
publisher Biodiversity Research Center, Academia Sinica
publishDate 2008
url http://ir.unimas.my/id/eprint/36019/1/natural1.pdf
http://ir.unimas.my/id/eprint/36019/
https://zoolstud.sinica.edu.tw/473.html
_version_ 1710679806804230144
score 13.211869

Similar Items