Cellulase Production by Aspergillus niger via Solid State Fermentation (SSF) using Agro-wastes
Agricultural-based industry is an important sector of economy for Malaysia as the geographical structure of this country is suitable for agricultural activities. However, the increase number of agricultural-based industries not only affects the economy positively, but also contributes towards env...
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2018
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/35391/2/Cellulase%20Production%20by%20Aspergillus%20niger%20via%20Solid%20State%20Fermentation%20%28SSF%29%20using%20Agro-wastes%28fulltext%29.pdf http://ir.unimas.my/id/eprint/35391/3/Shayreen%20ft.pdf http://ir.unimas.my/id/eprint/35391/ |
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| Summary: | Agricultural-based industry is an important sector of economy for Malaysia as the
geographical structure of this country is suitable for agricultural activities. However, the
increase number of agricultural-based industries not only affects the economy positively,
but also contributes towards environmental problems. Therefore, in this study, three types
of agro-wastes were utilized to produce cellulase enzyme via solid state fermentation to
reduce environmental pollution. The three types of agro-wastes were sago hampas,
pineapple leaf and coconut husk. Aspergillus niger was involved in this study to produce
cellulase because fungus is more suitable to be used in SSF compared to bacteria. Cellulase
are group of hydrolytic enzymes that have the ability to break down cellulose into simple
sugar. The cellulase activity was determined by using filter paper activity followed by
dinitrosalicylic acid method which were used to determine the amount of sugar produced.
SSF parameters such as incubation period, initial moisture content and temperature were
optimized to determine the highest cellulase activity. The results showed that pineapple
leaf is the best substrates for cellulase production by A. niger. |
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