Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba)

Neolamarckia cadamba (kelampayan) is an economically important tree in Malaysia as its demand in many industries are high due to its fast-growing property. In establishing tree improvement programme and management, numerous researchers had conducted studies on the contributing factors of the mech...

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Main Author: Tee, Alaythia Zhen Shi
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
Subjects:
Online Access:http://ir.unimas.my/id/eprint/35374/1/Alaythia%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/35374/4/Alaythia%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/35374/
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spelling my.unimas.ir.353742024-03-21T01:19:01Z http://ir.unimas.my/id/eprint/35374/ Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba) Tee, Alaythia Zhen Shi Q Science (General) SB Plant culture Neolamarckia cadamba (kelampayan) is an economically important tree in Malaysia as its demand in many industries are high due to its fast-growing property. In establishing tree improvement programme and management, numerous researchers had conducted studies on the contributing factors of the mechanism of wood fonnation ofkelampayan. In many plants, CLE genes are found to be crucial players in cell-to-cell communication that contributes to secondary plant growth and development. Few short and incomplete CLE genes available in the genomic database of kelampayan brought forth the need of studying and amplifying the partial genomic sequence of CLE genes in kelampayan. Several parameters of molecular techniques such as DNA extraction, PCR and AGE were used in perfonning this study. However, the PCR product sent for sequence analysis was found to be smearing". The reason for DNA smearing was carry-over contamination. Thus, the partial CLE gene sequence was not available for sequencing. Universiti Malaysia Sarawak, (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35374/1/Alaythia%2824pgs%29.pdf text en http://ir.unimas.my/id/eprint/35374/4/Alaythia%28fulltext%29.pdf Tee, Alaythia Zhen Shi (2018) Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba). [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic Q Science (General)
SB Plant culture
spellingShingle Q Science (General)
SB Plant culture
Tee, Alaythia Zhen Shi
Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba)
description Neolamarckia cadamba (kelampayan) is an economically important tree in Malaysia as its demand in many industries are high due to its fast-growing property. In establishing tree improvement programme and management, numerous researchers had conducted studies on the contributing factors of the mechanism of wood fonnation ofkelampayan. In many plants, CLE genes are found to be crucial players in cell-to-cell communication that contributes to secondary plant growth and development. Few short and incomplete CLE genes available in the genomic database of kelampayan brought forth the need of studying and amplifying the partial genomic sequence of CLE genes in kelampayan. Several parameters of molecular techniques such as DNA extraction, PCR and AGE were used in perfonning this study. However, the PCR product sent for sequence analysis was found to be smearing". The reason for DNA smearing was carry-over contamination. Thus, the partial CLE gene sequence was not available for sequencing.
format Final Year Project Report
author Tee, Alaythia Zhen Shi
author_facet Tee, Alaythia Zhen Shi
author_sort Tee, Alaythia Zhen Shi
title Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba)
title_short Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba)
title_full Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba)
title_fullStr Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba)
title_full_unstemmed Isolation and Sequence Analysis of CLE (CLAVATA3/Endosperm Surrounding Region-related) Gene in White Kelampayan (Neolamarckia cadamba)
title_sort isolation and sequence analysis of cle (clavata3/endosperm surrounding region-related) gene in white kelampayan (neolamarckia cadamba)
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/35374/1/Alaythia%2824pgs%29.pdf
http://ir.unimas.my/id/eprint/35374/4/Alaythia%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/35374/
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