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Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak

Staphylococcus aureus (S. aureus) is one of major human pathogen that able cause mild to serious infections. Present treatment becomes challenging to control due to the presence of methicillin-resistant Staphylococcus aureus (MRSA). The present study looks on the isolation and characterization of MR...

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Main Author: Nurul Nadia, Zayadi
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2018
Subjects:
SF Animal culture
Online Access:http://ir.unimas.my/id/eprint/35110/1/NURUL%20NADIA%2024%20pgs.pdf
http://ir.unimas.my/id/eprint/35110/5/NURUL%20NADIA%20ft.pdf
http://ir.unimas.my/id/eprint/35110/
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spelling my.unimas.ir.351102024-02-02T07:09:49Z http://ir.unimas.my/id/eprint/35110/ Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak Nurul Nadia, Zayadi SF Animal culture Staphylococcus aureus (S. aureus) is one of major human pathogen that able cause mild to serious infections. Present treatment becomes challenging to control due to the presence of methicillin-resistant Staphylococcus aureus (MRSA). The present study looks on the isolation and characterization of MRSA from air conditioning system in Faculty of Resources Science and Technology, UNIMAS. The swab samples were isolated from the ventilation of central air conditioner and dipped into 0.1 % peptone water. The swab samples were then carried out for serial dilution and plating on mannitol salt agar (MSA) and incubated for 24-36 hours in room temperature. Suspected colonies of S. aureus appeared as whitish colonies on MSA agar, and have gram-positive cocci in clusters. Only 16.67% of the total samples with dilution factor of 10-5 were positive for suspected S. aureus and were chosen to proceed for the following biochemical test. However, only 25% of the isolates were tested positive for catalase test, gelatin liquefaction test, and methyl red test, except for citrate test and urease test. All of the isolates were then carried out to assess their resistance towards four antibiotics by using disc diffusion method. All of the isolates were considered as resistant towards methicillin, penicillin, vancomycin, and cephalothin. This study is significant to increase awareness to UNIMAS staffs, lecturers, and students, by providing basic data for UNIMAS authorities on the status of MRSA from air conditioning system in UNIMAS. Universiti Malaysia Sarawak, (UNIMAS) 2018 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/35110/1/NURUL%20NADIA%2024%20pgs.pdf text en http://ir.unimas.my/id/eprint/35110/5/NURUL%20NADIA%20ft.pdf Nurul Nadia, Zayadi (2018) Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic SF Animal culture
spellingShingle SF Animal culture
Nurul Nadia, Zayadi
Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
description Staphylococcus aureus (S. aureus) is one of major human pathogen that able cause mild to serious infections. Present treatment becomes challenging to control due to the presence of methicillin-resistant Staphylococcus aureus (MRSA). The present study looks on the isolation and characterization of MRSA from air conditioning system in Faculty of Resources Science and Technology, UNIMAS. The swab samples were isolated from the ventilation of central air conditioner and dipped into 0.1 % peptone water. The swab samples were then carried out for serial dilution and plating on mannitol salt agar (MSA) and incubated for 24-36 hours in room temperature. Suspected colonies of S. aureus appeared as whitish colonies on MSA agar, and have gram-positive cocci in clusters. Only 16.67% of the total samples with dilution factor of 10-5 were positive for suspected S. aureus and were chosen to proceed for the following biochemical test. However, only 25% of the isolates were tested positive for catalase test, gelatin liquefaction test, and methyl red test, except for citrate test and urease test. All of the isolates were then carried out to assess their resistance towards four antibiotics by using disc diffusion method. All of the isolates were considered as resistant towards methicillin, penicillin, vancomycin, and cephalothin. This study is significant to increase awareness to UNIMAS staffs, lecturers, and students, by providing basic data for UNIMAS authorities on the status of MRSA from air conditioning system in UNIMAS.
format Final Year Project Report
author Nurul Nadia, Zayadi
author_facet Nurul Nadia, Zayadi
author_sort Nurul Nadia, Zayadi
title Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
title_short Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
title_full Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
title_fullStr Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
title_full_unstemmed Isolation and Characterization of Methicillin-Resistant Staphylococcus aureus from Air Conditioning System in Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
title_sort isolation and characterization of methicillin-resistant staphylococcus aureus from air conditioning system in faculty of resource science and technology, universiti malaysia sarawak
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2018
url http://ir.unimas.my/id/eprint/35110/1/NURUL%20NADIA%2024%20pgs.pdf
http://ir.unimas.my/id/eprint/35110/5/NURUL%20NADIA%20ft.pdf
http://ir.unimas.my/id/eprint/35110/
_version_ 1789945483225989120
score 13.211869

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