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Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak

Aims: Piper nigrum L. (black pepper) is an economically important commodity plant in Malaysia, which generated RM200.95 million from pepper export in the year of 2018. However, the increase in pepper production is restricted by diseases. Fusarium wilt is one of the major diseases of P. nigrum L. T...

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Main Authors: Tan, Wan Joo, Vu Thanh, Tu Anh, Rafael, Elissa Stella, Chen, Yi Shang, Yeo, Freddy, Kuok San
Format: Article
Language:English
Published: Malaysian Society for Microbiology 2021
Subjects:
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/34983/1/abstract.pdf
http://ir.unimas.my/id/eprint/34983/
https://mjm.usm.my/index.php
http://dx.doi.org/10.21161/mjm.200938
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spelling my.unimas.ir.349832021-04-01T02:54:07Z http://ir.unimas.my/id/eprint/34983/ Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak Tan, Wan Joo Vu Thanh, Tu Anh Rafael, Elissa Stella Chen, Yi Shang Yeo, Freddy, Kuok San S Agriculture (General) Aims: Piper nigrum L. (black pepper) is an economically important commodity plant in Malaysia, which generated RM200.95 million from pepper export in the year of 2018. However, the increase in pepper production is restricted by diseases. Fusarium wilt is one of the major diseases of P. nigrum L. The objectives for this study were to isolate Fusarium spp. associated with Fusarium wilt of P. nigrum L. from selected pepper farms in the northwestern region of Sarawak and to characterize the Fusarium spp. isolated morphologically and molecularly. Methodology and results: Fusarium spp. were isolated from diseased root samples. The pathogen was grown on potato dextrose agar (PDA) under dark condition at circa (ca.) 25 °C for morphological characterisation. Molecular characterisation was done by using internal transcribed spacer (ITS). Phylogenetic tree was constructed to study the genetic relationship of the isolates. Fusarium solani, F. oxysporum, F. proliferatum were the three Fusarium species identified. There were variations in morphological characters observed between and among the species, including the colony form, margin, elevation, surface appearance and pigmentation. No distinctive morphological characteristic was specific to a location. In addition, growth rate, macroconidia sporulation rate, and microconidia sporulation rate of the isolates were not correlated. In molecular phylogeny, the three Fusarium species were separated into three distinct clades representing the three identified species. The genetic relatedness between isolates within each species was depicted in the tree. Conclusion, significance and impact of study: Variations were observed among isolates in this study based on morphological and molecular characterization. This study would contribute information on the variations of Fusarium spp. associated with Fusarium wilt of P. nigrum L. from the northwestern region of Sarawak. Malaysian Society for Microbiology 2021-04-01 Article PeerReviewed text en http://ir.unimas.my/id/eprint/34983/1/abstract.pdf Tan, Wan Joo and Vu Thanh, Tu Anh and Rafael, Elissa Stella and Chen, Yi Shang and Yeo, Freddy, Kuok San (2021) Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak. Malaysian Journal of Microbiology, 17 (2). pp. 165-177. ISSN 2231-7538 https://mjm.usm.my/index.php http://dx.doi.org/10.21161/mjm.200938
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Tan, Wan Joo
Vu Thanh, Tu Anh
Rafael, Elissa Stella
Chen, Yi Shang
Yeo, Freddy, Kuok San
Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak
description Aims: Piper nigrum L. (black pepper) is an economically important commodity plant in Malaysia, which generated RM200.95 million from pepper export in the year of 2018. However, the increase in pepper production is restricted by diseases. Fusarium wilt is one of the major diseases of P. nigrum L. The objectives for this study were to isolate Fusarium spp. associated with Fusarium wilt of P. nigrum L. from selected pepper farms in the northwestern region of Sarawak and to characterize the Fusarium spp. isolated morphologically and molecularly. Methodology and results: Fusarium spp. were isolated from diseased root samples. The pathogen was grown on potato dextrose agar (PDA) under dark condition at circa (ca.) 25 °C for morphological characterisation. Molecular characterisation was done by using internal transcribed spacer (ITS). Phylogenetic tree was constructed to study the genetic relationship of the isolates. Fusarium solani, F. oxysporum, F. proliferatum were the three Fusarium species identified. There were variations in morphological characters observed between and among the species, including the colony form, margin, elevation, surface appearance and pigmentation. No distinctive morphological characteristic was specific to a location. In addition, growth rate, macroconidia sporulation rate, and microconidia sporulation rate of the isolates were not correlated. In molecular phylogeny, the three Fusarium species were separated into three distinct clades representing the three identified species. The genetic relatedness between isolates within each species was depicted in the tree. Conclusion, significance and impact of study: Variations were observed among isolates in this study based on morphological and molecular characterization. This study would contribute information on the variations of Fusarium spp. associated with Fusarium wilt of P. nigrum L. from the northwestern region of Sarawak.
format Article
author Tan, Wan Joo
Vu Thanh, Tu Anh
Rafael, Elissa Stella
Chen, Yi Shang
Yeo, Freddy, Kuok San
author_facet Tan, Wan Joo
Vu Thanh, Tu Anh
Rafael, Elissa Stella
Chen, Yi Shang
Yeo, Freddy, Kuok San
author_sort Tan, Wan Joo
title Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak
title_short Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak
title_full Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak
title_fullStr Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak
title_full_unstemmed Morphological and molecular characterization of Fusarium spp. associated with Fusarium wilt disease of Piper nigrum L. in Northwestern region of Sarawak
title_sort morphological and molecular characterization of fusarium spp. associated with fusarium wilt disease of piper nigrum l. in northwestern region of sarawak
publisher Malaysian Society for Microbiology
publishDate 2021
url http://ir.unimas.my/id/eprint/34983/1/abstract.pdf
http://ir.unimas.my/id/eprint/34983/
https://mjm.usm.my/index.php
http://dx.doi.org/10.21161/mjm.200938
_version_ 1696979551927664640
score 13.211869

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