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Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines

Ribosomes are essential component of ribosomal biogenesis and protein translation. However, it is also linked to the cancer development. Previous study shows that RPL37A is downregulated in nasopharyngeal carcinoma (NPC) cell lines compared to the normal nasopharyngeal cell line. Meanwhile, previou...

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Main Author: Liew, Jia Lee
Format: Final Year Project Report
Language:English
English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2014
Subjects:
QM Human anatomy
Online Access:http://ir.unimas.my/id/eprint/34365/3/Liew.pdf
http://ir.unimas.my/id/eprint/34365/6/Liew%20Jia%20Lee%20ft.pdf
http://ir.unimas.my/id/eprint/34365/
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spelling my.unimas.ir.343652024-03-01T03:12:11Z http://ir.unimas.my/id/eprint/34365/ Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines Liew, Jia Lee QM Human anatomy Ribosomes are essential component of ribosomal biogenesis and protein translation. However, it is also linked to the cancer development. Previous study shows that RPL37A is downregulated in nasopharyngeal carcinoma (NPC) cell lines compared to the normal nasopharyngeal cell line. Meanwhile, previous bioinformatics analysis revealed the interaction of Ribosomal Protein L37A (RPL37A) with beta-catenin (CTNNBI) and AT-binding transcription factor I (ATBFI). The participation and interaction of these genes in the NPC development remains uncertain due to lack of experimental evidence. In this study, comparative gene expression of RPL37A, CTNNBI and ATBF1 genes in NPC cell line compared to normal cell line is conducted by using Reverse-transcriptase PCR (RT-PCR). The down-regulation of RPL37A and ATBFI genes is observed in all of the NPC-derived cell lines when compared to normal cell line while CTNNBI is up-regulated in all of the NPC-derived cell lines. However, all of the expression shows no significant difference in the t-test (p>0.05). Universiti Malaysia Sarawak, (UNIMAS) 2014 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/34365/3/Liew.pdf text en http://ir.unimas.my/id/eprint/34365/6/Liew%20Jia%20Lee%20ft.pdf Liew, Jia Lee (2014) Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
English
topic QM Human anatomy
spellingShingle QM Human anatomy
Liew, Jia Lee
Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines
description Ribosomes are essential component of ribosomal biogenesis and protein translation. However, it is also linked to the cancer development. Previous study shows that RPL37A is downregulated in nasopharyngeal carcinoma (NPC) cell lines compared to the normal nasopharyngeal cell line. Meanwhile, previous bioinformatics analysis revealed the interaction of Ribosomal Protein L37A (RPL37A) with beta-catenin (CTNNBI) and AT-binding transcription factor I (ATBFI). The participation and interaction of these genes in the NPC development remains uncertain due to lack of experimental evidence. In this study, comparative gene expression of RPL37A, CTNNBI and ATBF1 genes in NPC cell line compared to normal cell line is conducted by using Reverse-transcriptase PCR (RT-PCR). The down-regulation of RPL37A and ATBFI genes is observed in all of the NPC-derived cell lines when compared to normal cell line while CTNNBI is up-regulated in all of the NPC-derived cell lines. However, all of the expression shows no significant difference in the t-test (p>0.05).
format Final Year Project Report
author Liew, Jia Lee
author_facet Liew, Jia Lee
author_sort Liew, Jia Lee
title Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines
title_short Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines
title_full Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines
title_fullStr Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines
title_full_unstemmed Characterization of the Expression Patterns of RPL37A, CTNNBI and ATBFI Genes in Normal and Tumour Human Cell Lines
title_sort characterization of the expression patterns of rpl37a, ctnnbi and atbfi genes in normal and tumour human cell lines
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2014
url http://ir.unimas.my/id/eprint/34365/3/Liew.pdf
http://ir.unimas.my/id/eprint/34365/6/Liew%20Jia%20Lee%20ft.pdf
http://ir.unimas.my/id/eprint/34365/
_version_ 1792658556496904192
score 13.211869

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