The preliminary studies of natural rubber serum (NRS) as a protein source for lactate fermentation
In the initial stage of the study, the effects of the different shaker speeds (l 00 rpm, 150 rpm and 200 rpm) in Ethanol Coagulation method were investigated. The result shows that there are no significant different between three volumes, however the brightness of the serum shows that 200rpm was s...
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2007
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/34097/2/The%20preliminary%20studies%20of%20natural%20rubber%20serum%20%28NRS%29%20as%20a%20protein%20source%20for%20lactate%20fermentation%28fulltext%29.pdf http://ir.unimas.my/id/eprint/34097/ |
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| Summary: | In the initial stage of the study, the effects of the different shaker speeds (l 00 rpm, 150 rpm and 200 rpm)
in Ethanol Coagulation method were investigated. The result shows that there are no significant different
between three volumes, however the brightness of the serum shows that 200rpm was suitable for ethanol
coagulation. The effects of different volumes of the Natural Rubber Serum ( I 0mJ, 20ml and 30mJ) as a
protein source were examined for lactate production using Lactococcus lactis IO-I. The batch fermentation
was carried out using shake flasks with initial pH adjusted at pH 6, temperature of 3 7"C and agitated at 200
rpm. The fermentation was conducted under controlled pH throughout the processes. The result obtained
was not significant to determine the accurate efficiency of any volumes ofNRS as a protein source due to
low amount of lactate produced. However, among the volumes studied, 30ml NRS shows higher
fermentation efficiency, 8.36% which 1.2% higher than both 10ml and 20 ml NRS respectively in
producing lactate. At the encl of the fermentation, 30mJ NRS was produced 1.85 g/L lactate with 8.36%
fermentation efficiency. |
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