Cover Image

Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method

Escherichia coli have caused several outbreaks of food borne illness. One of the main causes for this food borne illness is due to fresh vegetables particularly leafy greens. However in Malaysia, fresh vegetables have become one of Malaysian's main courses in their daily menu. Hence, the fresh...

Full description

Saved in:
Bibliographic Details
Main Author: Ailliyin Elmy, Ahmad Zaki
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2014
Subjects:
S Agriculture (General)
Online Access:http://ir.unimas.my/id/eprint/34014/1/Ailliyin%20Elmy%20Binti%20Ahmad%20Zaki%20ft.pdf
http://ir.unimas.my/id/eprint/34014/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimas.ir.34014
record_format eprints
spelling my.unimas.ir.340142023-02-21T04:33:20Z http://ir.unimas.my/id/eprint/34014/ Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method Ailliyin Elmy, Ahmad Zaki S Agriculture (General) Escherichia coli have caused several outbreaks of food borne illness. One of the main causes for this food borne illness is due to fresh vegetables particularly leafy greens. However in Malaysia, fresh vegetables have become one of Malaysian's main courses in their daily menu. Hence, the fresh vegetables need to be analysed to ensure that it is safe to be consumed by society. Therefore the main aim for this study is to molecular characterize E. coli isolated from fresh vegetables using ERIC- PCR. In this study, twenty one E. coli isolates which had been isolated previously from organic and conventional vegetables respectively were examined. Sixteen isolates shows the presence of E. coli on EMB agar. E. coli 0157: H7 was not detected in any of E. coli isolates. ERIC- PCR was used to determine the genetic relatedness of E. coli isolates due to its high discriminatory power. The 16 E. coli isolates were clustered into two major clusters (I and II). The organic vegetables were clustered in cluster I, while conventional vegetables were clustered in cluster II. Hence, the present study shows ERIC- PCR was able to group the E. coli isolates based on its source. Universiti Malaysia Sarawak, (UNIMAS) 2014 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/34014/1/Ailliyin%20Elmy%20Binti%20Ahmad%20Zaki%20ft.pdf Ailliyin Elmy, Ahmad Zaki (2014) Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Ailliyin Elmy, Ahmad Zaki
Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method
description Escherichia coli have caused several outbreaks of food borne illness. One of the main causes for this food borne illness is due to fresh vegetables particularly leafy greens. However in Malaysia, fresh vegetables have become one of Malaysian's main courses in their daily menu. Hence, the fresh vegetables need to be analysed to ensure that it is safe to be consumed by society. Therefore the main aim for this study is to molecular characterize E. coli isolated from fresh vegetables using ERIC- PCR. In this study, twenty one E. coli isolates which had been isolated previously from organic and conventional vegetables respectively were examined. Sixteen isolates shows the presence of E. coli on EMB agar. E. coli 0157: H7 was not detected in any of E. coli isolates. ERIC- PCR was used to determine the genetic relatedness of E. coli isolates due to its high discriminatory power. The 16 E. coli isolates were clustered into two major clusters (I and II). The organic vegetables were clustered in cluster I, while conventional vegetables were clustered in cluster II. Hence, the present study shows ERIC- PCR was able to group the E. coli isolates based on its source.
format Final Year Project Report
author Ailliyin Elmy, Ahmad Zaki
author_facet Ailliyin Elmy, Ahmad Zaki
author_sort Ailliyin Elmy, Ahmad Zaki
title Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method
title_short Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method
title_full Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method
title_fullStr Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method
title_full_unstemmed Molecular characterization of Escherichia coli isolated from fresh vegetables using ERIC- PCR method
title_sort molecular characterization of escherichia coli isolated from fresh vegetables using eric- pcr method
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2014
url http://ir.unimas.my/id/eprint/34014/1/Ailliyin%20Elmy%20Binti%20Ahmad%20Zaki%20ft.pdf
http://ir.unimas.my/id/eprint/34014/
_version_ 1758582564320182272
score 13.211869

Similar Items