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Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model

The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9)...

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Main Authors: Yu, Shu Pin, Ong, Kien Chai, Tan, Soon Hao, Perera, David, Wong, Kumthong
Format: Article
Language:English
Published: 2020
Subjects:
Q Science (General)
QR Microbiology
QR355 Virology
Online Access:http://ir.unimas.my/id/eprint/33613/1/shu.pdf
http://ir.unimas.my/id/eprint/33613/
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spelling my.unimas.ir.336132021-03-30T04:22:08Z http://ir.unimas.my/id/eprint/33613/ Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model Yu, Shu Pin Ong, Kien Chai Tan, Soon Hao Perera, David Wong, Kumthong Q Science (General) QR Microbiology QR355 Virology The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) derived from an autopsy brain. These biological clones were tested for neurovirulence in SK-N-MC and NIE-115 neuronal cells, and a 2-week-old, footpad-infected, JE mouse model. Nine clones were found to be neurovirulent; one clone neuroattenuated. Although further studies are needed to determine genotypic differences, if any, in these clones, the limiting dilution purification and neurovirulence testing methods described herein should be useful for phenotypic studies of quasispecies of neurotropic viruses in general, and JEV and other flaviviruses in particular. 2020-09 Article PeerReviewed text en http://ir.unimas.my/id/eprint/33613/1/shu.pdf Yu, Shu Pin and Ong, Kien Chai and Tan, Soon Hao and Perera, David and Wong, Kumthong (2020) Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model. Neurology Asia, 2 (3). pp. 279-284. ISSN 1823-6138 146154591
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QR Microbiology
QR355 Virology
spellingShingle Q Science (General)
QR Microbiology
QR355 Virology
Yu, Shu Pin
Ong, Kien Chai
Tan, Soon Hao
Perera, David
Wong, Kumthong
Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
description The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) derived from an autopsy brain. These biological clones were tested for neurovirulence in SK-N-MC and NIE-115 neuronal cells, and a 2-week-old, footpad-infected, JE mouse model. Nine clones were found to be neurovirulent; one clone neuroattenuated. Although further studies are needed to determine genotypic differences, if any, in these clones, the limiting dilution purification and neurovirulence testing methods described herein should be useful for phenotypic studies of quasispecies of neurotropic viruses in general, and JEV and other flaviviruses in particular.
format Article
author Yu, Shu Pin
Ong, Kien Chai
Tan, Soon Hao
Perera, David
Wong, Kumthong
author_facet Yu, Shu Pin
Ong, Kien Chai
Tan, Soon Hao
Perera, David
Wong, Kumthong
author_sort Yu, Shu Pin
title Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_short Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_full Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_fullStr Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_full_unstemmed Japanese encephalitis virus_Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
title_sort japanese encephalitis virus_biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model
publishDate 2020
url http://ir.unimas.my/id/eprint/33613/1/shu.pdf
http://ir.unimas.my/id/eprint/33613/
_version_ 1696979539326926848
score 13.211869

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