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Optimization of Small Scale Total Lipid Extraction from Frog Muscle

An optimized procedure based on Folch extraction method for extraction of total lipid from frog muscle is proposed. The effect of tissue disruption method (Hand grinding and glass beads), solvent chloroformmethanol ratio (2:1, 1:1 and 2.5:0.5), and sample-weight to solvent ratio (lg: 20ml, lg; 10ml...

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Bibliographic Details
Main Author: Nurul Adilah, Ismail
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2014
Subjects:
Q Science (General)
QL Zoology
Online Access:http://ir.unimas.my/id/eprint/33144/2/Nurul%20Adilah%20Binti%20Ismail%20ft.pdf
http://ir.unimas.my/id/eprint/33144/
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spelling my.unimas.ir.331442023-10-06T08:00:53Z http://ir.unimas.my/id/eprint/33144/ Optimization of Small Scale Total Lipid Extraction from Frog Muscle Nurul Adilah, Ismail Q Science (General) QL Zoology An optimized procedure based on Folch extraction method for extraction of total lipid from frog muscle is proposed. The effect of tissue disruption method (Hand grinding and glass beads), solvent chloroformmethanol ratio (2:1, 1:1 and 2.5:0.5), and sample-weight to solvent ratio (lg: 20ml, lg; 10ml, lg: 40ml) on percentage of lipid recovery was examined. Glass beads beating was shown to be the best method for frog muscle homogenization. Solvent ratio of 1: I was found to yield the highest percentage of lipid recovery. The percentage of lipid recovery was also recorded to be higher when using double volume of solvent. However, the result extracted by using standard Folch volume still falls within the frog lipid muscle range. When performing this extraction, the species of the frog should be maintained for each batch of optimization. It is essential to weigh the sample weight, and test tube weight accurately to avoid systemic error. Universiti Malaysia Sarawak, (UNIMAS) 2014 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/33144/2/Nurul%20Adilah%20Binti%20Ismail%20ft.pdf Nurul Adilah, Ismail (2014) Optimization of Small Scale Total Lipid Extraction from Frog Muscle. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QL Zoology
spellingShingle Q Science (General)
QL Zoology
Nurul Adilah, Ismail
Optimization of Small Scale Total Lipid Extraction from Frog Muscle
description An optimized procedure based on Folch extraction method for extraction of total lipid from frog muscle is proposed. The effect of tissue disruption method (Hand grinding and glass beads), solvent chloroformmethanol ratio (2:1, 1:1 and 2.5:0.5), and sample-weight to solvent ratio (lg: 20ml, lg; 10ml, lg: 40ml) on percentage of lipid recovery was examined. Glass beads beating was shown to be the best method for frog muscle homogenization. Solvent ratio of 1: I was found to yield the highest percentage of lipid recovery. The percentage of lipid recovery was also recorded to be higher when using double volume of solvent. However, the result extracted by using standard Folch volume still falls within the frog lipid muscle range. When performing this extraction, the species of the frog should be maintained for each batch of optimization. It is essential to weigh the sample weight, and test tube weight accurately to avoid systemic error.
format Final Year Project Report
author Nurul Adilah, Ismail
author_facet Nurul Adilah, Ismail
author_sort Nurul Adilah, Ismail
title Optimization of Small Scale Total Lipid Extraction from Frog Muscle
title_short Optimization of Small Scale Total Lipid Extraction from Frog Muscle
title_full Optimization of Small Scale Total Lipid Extraction from Frog Muscle
title_fullStr Optimization of Small Scale Total Lipid Extraction from Frog Muscle
title_full_unstemmed Optimization of Small Scale Total Lipid Extraction from Frog Muscle
title_sort optimization of small scale total lipid extraction from frog muscle
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2014
url http://ir.unimas.my/id/eprint/33144/2/Nurul%20Adilah%20Binti%20Ismail%20ft.pdf
http://ir.unimas.my/id/eprint/33144/
_version_ 1779150701927071744
score 13.211869

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