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Screening and Isolation of lignocellulolytic fungi from mangrove soils

Lignocellulolytic fungi play an important role in bioremediation of many environmentally persistent pollutants such as aromatic xenobiotics, heterocyclic aromatic hydrocarbons, chlorinated aromatic compounds and various dyes which are used in textile industries and pulping industries. Lignin is a c...

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Main Author: Soo, Hui Yin
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2013
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/3273/3/Soo%20Hui%20Yin%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/3273/
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spelling my.unimas.ir.32732024-02-06T03:24:45Z http://ir.unimas.my/id/eprint/3273/ Screening and Isolation of lignocellulolytic fungi from mangrove soils Soo, Hui Yin Q Science (General) Lignocellulolytic fungi play an important role in bioremediation of many environmentally persistent pollutants such as aromatic xenobiotics, heterocyclic aromatic hydrocarbons, chlorinated aromatic compounds and various dyes which are used in textile industries and pulping industries. Lignin is a compound which is held together by strong chemical bonding. This made it difficult to be degraded.However, studies had found that, lignocellulolytic fungi can solve these environmental problems by secreting their nonspecific lignocellulolytic extracellular enzymes. In this research, a lignocellulolytic fungus SHY 43 was isolated from mangrove soils and was identified as Penicilium sp. It showed 92.6 % of Remazol Brilliant Blue R dye decolourisation which indicates the presence of ligninolytic activities.It also performed well in cellulase screening test whereby it had the highest total endoglucanase activity on day 8 which was 3.074 U/L. It was then used in the solid state fermentation (SSF) using oil palm empty fruit bunch (OPEFB) as the substrate. There were four enzymes analysed during the enzyme assays, which were laccase, manganese peroxidase, lignin peroxidase and cellulase. Among these four enzymes, only cellulase activity was detected whereas the other three ligninolytic enzymes were deactivated. Total endoglucanase activity peaked at day 9 with a value of 0.067 U/ml. Universiti Malaysia Sarawak, (UNIMAS) 2013 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/3273/3/Soo%20Hui%20Yin%20%28fulltext%29.pdf Soo, Hui Yin (2013) Screening and Isolation of lignocellulolytic fungi from mangrove soils. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Soo, Hui Yin
Screening and Isolation of lignocellulolytic fungi from mangrove soils
description Lignocellulolytic fungi play an important role in bioremediation of many environmentally persistent pollutants such as aromatic xenobiotics, heterocyclic aromatic hydrocarbons, chlorinated aromatic compounds and various dyes which are used in textile industries and pulping industries. Lignin is a compound which is held together by strong chemical bonding. This made it difficult to be degraded.However, studies had found that, lignocellulolytic fungi can solve these environmental problems by secreting their nonspecific lignocellulolytic extracellular enzymes. In this research, a lignocellulolytic fungus SHY 43 was isolated from mangrove soils and was identified as Penicilium sp. It showed 92.6 % of Remazol Brilliant Blue R dye decolourisation which indicates the presence of ligninolytic activities.It also performed well in cellulase screening test whereby it had the highest total endoglucanase activity on day 8 which was 3.074 U/L. It was then used in the solid state fermentation (SSF) using oil palm empty fruit bunch (OPEFB) as the substrate. There were four enzymes analysed during the enzyme assays, which were laccase, manganese peroxidase, lignin peroxidase and cellulase. Among these four enzymes, only cellulase activity was detected whereas the other three ligninolytic enzymes were deactivated. Total endoglucanase activity peaked at day 9 with a value of 0.067 U/ml.
format Final Year Project Report
author Soo, Hui Yin
author_facet Soo, Hui Yin
author_sort Soo, Hui Yin
title Screening and Isolation of lignocellulolytic fungi from mangrove soils
title_short Screening and Isolation of lignocellulolytic fungi from mangrove soils
title_full Screening and Isolation of lignocellulolytic fungi from mangrove soils
title_fullStr Screening and Isolation of lignocellulolytic fungi from mangrove soils
title_full_unstemmed Screening and Isolation of lignocellulolytic fungi from mangrove soils
title_sort screening and isolation of lignocellulolytic fungi from mangrove soils
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2013
url http://ir.unimas.my/id/eprint/3273/3/Soo%20Hui%20Yin%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/3273/
_version_ 1792160555845287936
score 13.211869

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