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Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress

Hyper- and hypothermia are utilized as treatment modalities in cancer treatment or as a protection against ischemia-reperfusion induced cell damage. The under-lying mechanism of hyper- and hypothermia, on cell death in osteosarcoma cells are not well understood. The aim of this study is to investiga...

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Main Authors: Mohammed Ali, Nashiry, AL-Khateeb, Alyaa R, Yeap, S Keong, Froemming, Gabriele R A
Format: Article
Language:English
Published: Penerbit UTM Press 2020
Subjects:
Q Science (General)
Online Access:http://ir.unimas.my/id/eprint/32718/1/Gabriele.pdf
http://ir.unimas.my/id/eprint/32718/
https://mjfas.utm.my/index.php/mjfas/article/view/1344
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spelling my.unimas.ir.327182021-04-01T08:34:56Z http://ir.unimas.my/id/eprint/32718/ Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress Mohammed Ali, Nashiry AL-Khateeb, Alyaa R Yeap, S Keong Froemming, Gabriele R A Q Science (General) Hyper- and hypothermia are utilized as treatment modalities in cancer treatment or as a protection against ischemia-reperfusion induced cell damage. The under-lying mechanism of hyper- and hypothermia, on cell death in osteosarcoma cells are not well understood. The aim of this study is to investigate the short- and long-term effects of various severities of hyper- and hypothermia on osteoblast-like osteosarcoma cells (MG-63). MG-63 cells were treated with mild and severe hyper- and hypothermia for short, medium and long-term periods. Severe hypothermia and hyperthermia showed a time-dependent toxicity; hence viability was reduced in a significant manner at all time points and the cells were undergoing apoptosis. Mild hypothermia, on the other hand, showed a protective effect and long term exposure increased the cell viability. Severe hyperthermia induced significant DNA damage at all time points. Caspase 3/7 activity showed a significant increase at 1 h of severe hyperthermia and 72 h of severe hypothermia (p<0.05). Hsp90 expression was significantly increased at 72 h of mild hyperthermia (p<0.01), whereas Hsp70 showed a significant increase after 24 and 72 h (p<0.01 and p<0.001). Hsp27 mRNA was increased significantly at 24 h only under mild hyperthermia (p<0.01). In conclusion, hyperthermia especially severe hyperthermia induced cellular stress in MG-63 cells leading to apoptosis. Hypothermia, on the other hand caused severe cell stress only when the cells were challenged for a prolonged period with severe low temperatures. Penerbit UTM Press 2020 Article PeerReviewed text en http://ir.unimas.my/id/eprint/32718/1/Gabriele.pdf Mohammed Ali, Nashiry and AL-Khateeb, Alyaa R and Yeap, S Keong and Froemming, Gabriele R A (2020) Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress. Malaysian Journal of Fundamental and Applied Sciences, 16 (5). pp. 585-596. ISSN 2289-599X https://mjfas.utm.my/index.php/mjfas/article/view/1344
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Mohammed Ali, Nashiry
AL-Khateeb, Alyaa R
Yeap, S Keong
Froemming, Gabriele R A
Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress
description Hyper- and hypothermia are utilized as treatment modalities in cancer treatment or as a protection against ischemia-reperfusion induced cell damage. The under-lying mechanism of hyper- and hypothermia, on cell death in osteosarcoma cells are not well understood. The aim of this study is to investigate the short- and long-term effects of various severities of hyper- and hypothermia on osteoblast-like osteosarcoma cells (MG-63). MG-63 cells were treated with mild and severe hyper- and hypothermia for short, medium and long-term periods. Severe hypothermia and hyperthermia showed a time-dependent toxicity; hence viability was reduced in a significant manner at all time points and the cells were undergoing apoptosis. Mild hypothermia, on the other hand, showed a protective effect and long term exposure increased the cell viability. Severe hyperthermia induced significant DNA damage at all time points. Caspase 3/7 activity showed a significant increase at 1 h of severe hyperthermia and 72 h of severe hypothermia (p<0.05). Hsp90 expression was significantly increased at 72 h of mild hyperthermia (p<0.01), whereas Hsp70 showed a significant increase after 24 and 72 h (p<0.01 and p<0.001). Hsp27 mRNA was increased significantly at 24 h only under mild hyperthermia (p<0.01). In conclusion, hyperthermia especially severe hyperthermia induced cellular stress in MG-63 cells leading to apoptosis. Hypothermia, on the other hand caused severe cell stress only when the cells were challenged for a prolonged period with severe low temperatures.
format Article
author Mohammed Ali, Nashiry
AL-Khateeb, Alyaa R
Yeap, S Keong
Froemming, Gabriele R A
author_facet Mohammed Ali, Nashiry
AL-Khateeb, Alyaa R
Yeap, S Keong
Froemming, Gabriele R A
author_sort Mohammed Ali, Nashiry
title Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress
title_short Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress
title_full Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress
title_fullStr Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress
title_full_unstemmed Human MG-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress
title_sort human mg-63 osteosarcoma cells responses to long and short term hyper- and hypothermia stress
publisher Penerbit UTM Press
publishDate 2020
url http://ir.unimas.my/id/eprint/32718/1/Gabriele.pdf
http://ir.unimas.my/id/eprint/32718/
https://mjfas.utm.my/index.php/mjfas/article/view/1344
_version_ 1696979530538811392
score 13.211869

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